BID (NM_197966) Human 3' UTR Clone

CAT#: SC214685

3`UTR clone of BH3 interacting domain death agonist (BID) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: BID
• Synonyms: FP497
• ACCN: NM_197966
• Insert Size: 1459 bp
• Sequence Data:
  Insert Sequence

  >SC214685 3'UTR clone of NM_197966
  The sequence shown below is from the reference sequence of NM_197966. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACCTACGTGAGGAGCTTAGCCAGAAATGGGATGGACTGAACGGACAGTTCCAGAAGTGTGACTGGCTAAA
  GCTCGATGTGGTCACAGCTGTATAGCTGCTTCCAGTGTAGACGGAGCCCTGGCATGTCAACAGCGTTCCT
  AGAGAAGACAGGCTGGAAGATAGCTGTGACTTCTATTTTAAAGACAATGTTAAACTTATAACCCACTTTA
  AAATATCTACATTAATATACTTGAATGAAAATGTCCATTTACACGTATTTGAATGGCCTTCATATCATCC
  ACACATGAATCTGCACATCTGTAAATCTACACACGGTGCCTTTATTTCCACTGTGCAGGTTCCCACTTAA
  AAATTAAATTGGAAAGCAGGTTTCAAGGAAGTAGAAACAAAATACAATTTTTTTGGTAAAAAAAAATTAC
  TGTTTATTAAAGTACAACCATAGAGGATGGTCTTACAGCAGGCAGTATCCTGTTTGAGGAAAGCAAGAAT
  CAGAGAAGGAACATACCCCTTACAAATGAAAAATTCCACTCAAAATAGGGACTATCTATCTTAATACTAA
  GGAACCAACAATCTTCCTGTTTAAAAAACCACATGGCACAGAGATTCTGAACTAAAGTGCTGCACTCAAA
  TGATGGGAAGTCCGGCCCCAGTACACAGGGGCTTGACTTTTTCAACTTCGTTTCCTTTGTTGGAGTCAAA
  AAGAACCACTTGTGGTTCTAAAAGGTGTGAAGGTGATTTAAGGGCCCAGGTCAGCCACTGTTTGTTTACA
  AAATCAGGTAACTAACTGCATACACTTTTTCTCTTTCCATGACATCAAGACTTTGCTAAAGACATGAAGC
  CACGGGTGCCAGAAGCTACTGCGATGCCCCGGGAGTTAGCCCCCTGGTAATAGCTGTAAACTTCCAATTT
  CTAGCCATACGCTCAGCTCATCCATGCCTCAGAAGTGCATCTGGAGAGAACAGGTTTCTAAGCATAAAAG
  ATGAAAGAGCAGTTGGACTTTTTAAAAATTCAGCAAAGTGGTTCCCTCTCTTAGGGACAGTCAAAACCAA
  GTCACTTAGGTAGTACCAAAATAAATAAGGAAAAGCTTAGCTTTAGAAACAGTGCAACACTGGTCTGCTG
  TTCCAGTGGTAAGCTATGTCCCAGGAATCAGTTTAAAAGCACGACAGTGGATGCTGGGTCCATATCACAC
  ACATTGCTGTGAACAGGAAACTCCTGTGACCACAACATGAGGCCACTGGAGACGCATATGAGTAAGGGCA
  CTGACGGACTCATGATTTCTTCTTACCAGATGCTTTCCTGTTCTTTAAGAGTTTAAAATCATCAGAAAGG
  AAAAACAAACTCTATATTGTTCAGCATGCAATACATACCACGCTAGGGCTGGCTCAATTGAAAGTGGGCA
  AAAGCTTACAAATACTAAAAAGAAGTGCTGCCGCGCAGTGTGGAGGCCACTGTTTGGAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_197966.1
• Summary: 'This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Aug 2020]'
• Locus ID: 637