Caspase-7 (CASP7) (NM_033340) Human 3' UTR Clone

CAT#: SC214921

3`UTR clone of caspase 7 apoptosis-related cysteine peptidase (CASP7) transcript variant beta for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CASP7
• Synonyms: CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3
• ACCN: NM_033340
• Insert Size: 1524 bp
• Sequence Data:
  Insert Sequence

  >SC214921 3'UTR clone of NM_033340
  The sequence shown below is from the reference sequence of NM_033340. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGCACGGAAAAGACCTGGAAATCATGCAGATCCTCACCAGGGTGAATGACAGAGTTGCCAGGCACTTTGA
  GTCTCAGTCTGATGACCCACACTTCCATGAGAAGAAGCAGATCCCCTGTGTGGTCTCCATGCTCACCAAG
  GAACTCTACTTCAGTCAATAGCCATATCAGGGGTACATTCTAGCTGAGAAGCAATGGGTCACTCATTAAT
  GAATCACATTTTTTTATGCTCTTGAAATATTCAGAAATTCTCCAGGATTTTAATTTCAGGAAAATGTATT
  GATTCAACAGGGAAGAAACTTTCTGGTGCTGTCTTTTGTTCTCTGAATTTTCAGAGACTTTTTTTATAAT
  GTTATTCATTTGGTGACTGTGTAACTTTCTCTTAAGATTAATTTTCTCTTTGTATGTCTGTTACCTTGTT
  AATAGACTTAATACATGCAACAGAAGTGACTTCTGGAGAAAGCTCATGGCTGTGTCCACTGCAATTGGTG
  GTAACAGTGGTAGAGTCATGTTTGCACTTGGCAAAAAGAATCCCAATGTTTGACAAAACACAGCCAAGGG
  GATATTTACTGCTCTTTATTGCAGAATGTGGGTATTGAGTGTGATTTGAATGATTTTTCATTGGCTTAGG
  GCAGATTTTCATGCAAAAGTTCTCATATGAGTTAGAGGAGAAAAAGCTTAATGATTCTGATATGTATCCA
  TCAGGATCCAGTCTGGAAAACAGAAACCATTCTAGGTGTTTCAACAGAGGGAGTTTAATACAGGAAATTG
  ACTTACATAGATGATAAAAGAGAAGCCAAACAGCAAGAAGCTGTTACCACACCCAGGGCTATGAGGATAA
  TGGGAAGAGGTTTGGTTTCCTGTGTCCAGTAGTGGGATCATCCAGAGGAGCTGGAACCATGGTGGGGGCT
  GCCTAGTGGGAGTTAGGACCACCAATGGATTGTGGAAAATGGAGCCATGACAAGAACAAAGCCACTGACT
  GAGATGGAGTGAGCTGAGACAGATAAGAGAATACCTTGGTCTCACCTATCCTGCCCTCACATCTTCCACC
  AGCACCTTACTGCCCAGGCCTATCTGGAAGCCACCTCACCAAGGACCTTGGAAGAGCAAGGGACAGTGAG
  GCAGGAGAAGAACAAGAAATGGATGTAAGCCTGGCCCATAATGTGAACATAAGTAATCACTAATGCTCAA
  CAATTTATCCATTCAATCATTTATTCATTGGGTTGTCAGATAGTCTATGTATGTGTAAAACAATCTGTTT
  TGGCTTTATGTGCAAAATCTGTTATAGCTTTAAAATATATCTGGAACTTTTTAGATTATTCCAAGCCTTA
  TTTTGAGTAAATATTTGTTACTTTTAGTTCTATAAGTGAGGAAGAGTTTATGGCAAAGATTTTTGGCACT
  TTGTTTTCAAGATGGTGTTATCTTTTGAATTCTTGATAAATGACTGTTTTTTTCTGCCTAATAGTAACTG
  GTTAAAAAACAAATGTTCATATTTATTGATTAAAAATGTGGTTGCTTAATTCCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_033340.2
• Summary: 'This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]'
• Locus ID: 840