PPP2R4 (PTPA) (NM_178000) Human 3' UTR Clone

CAT#: SC214922

3`UTR clone of protein phosphatase 2A activator regulatory subunit 4 (PPP2R4) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PTPA
• Synonyms: PP2A; PPP2R4; PR53
• ACCN: NM_178000
• Insert Size: 1503 bp
• Sequence Data:
  Insert Sequence

  >SC214922 3'UTR clone of NM_178000
  The sequence shown below is from the reference sequence of NM_178000. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATCCATCCTGTCACGTCGGGCTAGGAGGGGCCAAGCCGAAGAGCCACCCAGGCCACAGTTCCTGTGCCTG
  CCTTCCCCACCCCAGCAGTGGCCCCTCCCCATCCCCTCCCTCTGTTCGTCCCGTTTGATGAGAGGCTGTT
  TACTGGGGTGGGGTGGCGAGATGGGCTTGAGGGGGCTCAGAGCATAAGGCTTCAGGGCCCAAGTTGGGAG
  AAGTGACCAAAGTGTAGCCAGTTTTCTGAGTTCCCGTGTGCTAGACTGGCCAGAAGAGAGGGTCTGGGGC
  CTGGTCACTCGGCCACTCTCTCCTGTTTCTGGCCTCTTCTCCCTTCACTCCCGTCCAGTCTGGTTTTGAG
  AGCAGGGGCTGTTCTGCAGCACCGCAGGGAAGGGAGGAGAGATACCTGCTGCTTCCATTGCTTTTCCCTT
  CCTGGAGTCGATGCCTTTCTAAGGGTTGGAGCTGCTCCTTGCAGGGGCGGGTCAGTTTCCCAGGCCATGC
  CGGGGTGGCCATCTATGGTAGGGCTGGAAGCTGAGGCTGGCCGCCAGCTGTGGGCTGGGGTGGGGTGGGT
  GGGGTCGGGTGGTGGAGAGGCCTTAGCTGTCCTGGCTGGTGCCCCTCCCAGGCTCCTTTTCACCCTGCCC
  CCTGGGCCTGAGGCCCCCTGTGTCCAAGCCTCCCCCTGGCTCTTCAGTTCTCTAGCCCTTGGCTCTGCTG
  GGTTTCCTGACTGTAGCCACATCTCTCCCGCTCCCTAAGGGTAACCTAGCCAATGGAAGCTGCCCTTTGG
  GTAGGTGCTGGGCTCCTGGGAGGGCCCAGATGATGGGGTGAGGCATGTCTTTCCAGAACTTTCCCTGGCA
  GGGAGGGGATGGCAGAAACTCAGGGAGGGGCTTGGGGCCCATTGTATCTGGAGAGCCTGGATTCCTCTTG
  GCAGTCTTAGGCCCGGCCACTTCTGCTACCTTTGCGCTGCTGTGAGCCTCACCCTGGGCCCCTGGGCCCT
  GCTTCTCTGCTCCCCTGGGTGATGGGTGGGCCCAGAAGGTGGCAGTCCCACACCTTGTCCTCCCACCTCC
  CTGAACTGTCCATTGCTTTTATAGGGTGAGGTAAGAGACAGCCTCCCAAGCCCAGGCTTTGGCACTCAGA
  ATGGGCCCAGTGGGGGCTGGGCAGGCCCATTGAGGGCCACCGCCGAGGTTTCTCCTAGGGCTGTTCCTGG
  GCCTGGCTCTTACAGGCTCGTCCCCCAGGCCTGCCCTTCTCCACTGCCCCCTCCTGTGTCTGGGTCCACA
  CACCCTTCAGGAAGGGGGAGCACTGAGAAGCACAGCACAGGGGCTCAGCCTGGGATCCGGTGATGGTCTG
  GGCAGAGGCTGGGTCAGGAGTCCCAAAGGTCAGTGACAGTTTCTCAGAAGAGGCCCAGCGTCCACCTCTC
  TCCCAGGGCCAGACAGCCCTTCCTGGCTCCCCCATCCCCCTATGGGCTCCCAGCCCCTTGCACCCTCATT
  GCTGTTCAGATTAAAGCCTCTGTTTTGCACCTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_178000.1
• Summary: 'Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
• Locus ID: 5524