MAP4K5 (NM_198794) Human 3' UTR Clone

CAT#: SC215010

3`UTR clone of mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MAP4K5
• Synonyms: GCKR; KHS; KHS1; MAPKKKK5
• ACCN: NM_198794
• Insert Size: 1506
• Sequence Data:
  Insert Sequence

  >SC215010 3'UTR clone of NM_198794
  The sequence shown below is from the reference sequence of NM_198794. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTCTACATCTTGGCTGGACATGAAAATAGTTACTAAGCAACAGAAACTGATCTCAAATGACAGGAAAATG
  AATATACTCCATTGAAAGGAAAAATAAGGAAATTCAATACAAACTGCACTATGATTTGCTTTAACTATTA
  TGGGTTATATTGCAAATGATCTGTACTTTAGGGTAGAATTCAATATTTTCTGCAGCTGGAAACAGCTAGT
  CTATCTCTTGCCACTGTGTGGTGGTTATATCAAGTTTGCTTAATAAAAGCTATGAGACAAATAGTCCTCT
  AGTTCCAGGAAACACAGTCTTTTTTTAAAAAAAACAATGTTTGTAACAAGGGTGCCATGGTATTTTTAGA
  TAACTCGTGATTATCTTAAGAGAGGTAAATTTAGTGATCATTTTATATCATGTCTTATTCCTTCTTAATG
  AACATAATTTGTTAAATTCTCAAGCAAGGTTTTCACTTTTATATTGGCCATTCTGTATGTTTTTGTAAAA
  CAGAATATTTAATCCTTATTTATTAATCTCTTGCTGGAGTGGTGTAATGTATCTAACTTTTAGCAAAGGA
  GGGTTGCAGAGCAGCTTAAATTTTTTTTATAATGTATAAGAATTTTGTTTATCTTTTAAGAGTAGTAAAG
  TACTTTGAGTGTTTGGGGGTTCAACACACACATGCAATTTTGCTTAACAAAAGTATTTTATAATACAGTT
  TCATACAGAATTACCTTAAAAGGGAGTCTTATGTTTTCAACTACAGATAGTTGTAAGGGATCATACAGAA
  GATATTGATGATAGTTGAAATATTCTTAGAAGGGGTGTGTATGTCTAGCTGTGTCTACCATGTGTATGTA
  TTCTTGACAAGCAGTATAAAATACCTGTGATTTTTCTTTACATTAGGGATAATGCATAAGGAATTAATCT
  TCATATATATTATCATCCCTAATGTAGCAGGGGGAAGTATTTAATTGCCCATGATATGTATTTTACTTAT
  ACTATGCCAGAGAGGAAACTATAAAGTAATTACACATGTAATCTTGGGTTTTTCACATATGTAGGTATTC
  ATTTTGAGTAGGTTGAAGAAGAAAAAAAATATTTAAATGAATTGAATTCCTGATGGGATAGTATCAATAA
  GTATTTAAAAGCCAGTATTCTAAAAATAATAAAGGGTAGGGTCATTTTTGAGTTTGTTTTTCTTTTGCTA
  TTGTTAATATTCAAAATTAAAGTGTTACATTGGTACCTGTTGTCTTAATGCATTTATTGAGAACAGCATT
  GAGATGATGAACAAGGGGTTAGCAATAGCAAACTCTATAATTATTTTGACTAATTACTTAAGAGGAAAAC
  AGTATAAGTATCTCATTCAGTATTTAGCAATTCTGTAAAATAAGTATTATCTCTATTTTTCAGATGAGGA
  AGTAAGGGTTTAGCAAGGTTAAGAGATCTATCCAATTTACACAGCAAGTTAGTAGTTGAGCCTGACCATG
  AGTCTTCTGACTCTGTTCTTTTCACTATGCAATACG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_198794.1
• Summary: This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 11183