CD11a (ITGAL) (NM_002209) Human 3' UTR Clone

CAT#: SC215161

3`UTR clone of integrin alpha L (antigen CD11A (p180) lymphocyte function-associated antigen 1; alpha polypeptide) (ITGAL) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ITGAL
• Synonyms: CD11A; LFA-1; LFA1A
• ACCN: NM_002209
• Insert Size: 1527 bp
• Sequence Data:
  Insert Sequence

  >SC215161 3'UTR clone of NM_002209
  The sequence shown below is from the reference sequence of NM_002209. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAGAAGGACTCTGAGAGTGGTGGTGGCAAGGACTGAGTCCAGGCCTGTGAGGTGCAGAGTGCCCAGAACT
  GGACTCAGGATGCCCAGGGCCACTCTGCCTCTGCCTGCATTCTGCCGTGTGCCCTCGGGCGAGTCACTGC
  CTCTCCCTGGCCCTCAGTTTCCCTATCTCGAACATGGAACTCATTCCTGCCTGTCTCCTTTGCAGGCTCA
  TAGGGAAGACCTGCTGAGGGACCAGCCAAGAGGGCTGCAAAAGTGAGGGCTTGTCATTACCAGACGGTTC
  ACCAGCCTCTCTTGGTTTCCTTCCTTGGAAGAGAATGTCTGATCTAAATGTGGAGAAACTGTAGTCTCAG
  GACCTAGGGATGTTCTGGCCCTCACCCCTGCCCTGGGATGTCCACAGATGCCTCCACCCCCCAGAACCTG
  TCCTTGCACACTCCCCTGCACTGGAGTCCAGTCTCTTCTGCTGGCAGAAAGCAAATGTGACCTGTGTCAC
  TACGTGACTGTGGCACACGCCTTGTTCTTGGCCAAAGACCAAATTCCTTGGCATGCCTTCCAGCACCCTG
  CAAAATGAGACCCTCGTGGCCTTCCCCAGCCTCTTCTAGAGCCGTGATGCCTCCCTGTTGAAGCTCTGGT
  GACACCAGCCTTTCTCCCAGGCCAGGCTCCTTCCTGTCTTCCTGCATTCACCCAGACAGCTCCCTCTGCC
  TGAACCTTCCATCTCGCCACCCCTCCTTCCTTGACCAGCAGATCCCAGCTCACGTCACACTTGGTTGGGT
  CCTCACATCTTTCACACTTCCACCAGCCTGCACTACTCCCTCAAAGCACACGTCATGTTTCTTCATCCGG
  CAGCCTGGATGTTTTTTCCCTGTTTAATGATTGACGTACTTAGCAGCTATCTCTCAGTGAACTGTGAGGG
  TAAAGGCTATACTTGTCTTGTTCACCTTGGGATGATGCCTCATGATATGTCAGGGCGTGGGACATCTAGT
  AGGTGCTTGACATAATTTCACTGAATTAATGACAGAGCCAGTGGGAAGATACAGAAAAAGAGGGGCTGGG
  CTGGGCGCGGTGGTTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGAGGGTGGATCACCTGAGGTC
  AGGAGTTAGAGGCCAGCCTGGCGAAACCCCATCTCTACTAAAAATACAAAATCCAGGCGTGGTGGCACAC
  ACCTGTAGTCCCAGCTACTCAGGAGGTTGAGGTAGGAGAATTGCTTGAACCTGGGAGGTGGAGGTTGCAG
  TGAGCCAAGATTGCGCCATTGCACTCCAGCCTGGGCAACACAGCGAGACTCCGTCTCAAGGAAAAAATAA
  AAATAAAAAGCGGGCACGGGCCCGTGACATCCCCACCCTTGGAGGCTGTCTTCTCAGGCTCTGCCCTGCC
  CTAGCTCCACACCCTCTCCCAGGACCCATCACGCCTGTGCAGTGGCCCCCACAGAAAGACTGAGCTCAAG
  GTGGGAACCACGTCTGCTAACTTGGAGCCCCAGTGCCAAGCACAGTGCCTGCATGTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002209.2
• Summary: 'ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 3683