RFX5 (NM_000449) Human 3' UTR Clone

CAT#: SC215217

3`UTR clone of regulatory factor X 5 (influences HLA class II expression) (RFX5) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RFX5
• ACCN: NM_000449
• Insert Size: 1554 bp
• Sequence Data:
  Insert Sequence

  >SC215217 3'UTR clone of NM_000449
  The sequence shown below is from the reference sequence of NM_000449. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAGGAGCATAAAGACCCAAAAGCAACACCCCCATGATACAGGTCTGTGGGGAAGAGTGTTTATATCCCT
  ACGTTAACTTTGCCTAGTAGAGGCCCTTCTTTGCACTTGCTTCTCATTTGGCTATTCTTTTCCTAAGGAA
  GTCCATTCTCCTCTGTACAGACAGCTGAGTCACCCAGTCTACTTAGTACCTGGTTGCTGCCTCTGACCTT
  TTCAGCTTGATACCCTGGGCTTTAGTGTAACCAATAAATCTGTAGTGACCTTACCTGTATTCCCTGTGCT
  ATCCTGTGGGAAGGTAGGAATGGGCTAAGTATGATGAATATATAGGTTAGGGATCTTTTGGTTTTAAATC
  ACAGAAAACCTAATTCAAACTGGCTTAAAATAAAAAGGATTTATTGGTTCATGTAACTAGAAAGTCCATA
  GGTAGTGCTGGCTCCAGGTGAAGACTTGACCCAGTAGTTCAGTATGTCTCTAAATACCGGACTGACTTTT
  TTCTCACTGTTGCATCTTCTGTAGGACCATTTAAGTCTGGGCCACTTAATGGCTGCCAGCATTCCTAAGA
  TTACACTTTTCCCCATTTATGTCCAATCAGAAAAAGAAGGCATCTTTGTACCAGAAATCTCAGCAAAAGC
  CCTAATATTCACACTGATTAGGCCTGGGTCACATGTCCACCCTGACCAATCACTGTGGCCAGGAGGATGA
  TACATGCTAATTTGCTTATTCTATATCATGGACAACACCTTTGGGGAAAAGGGTGGGGGTCAGCCTCCCC
  AAAATCACATGGATTCCCCAAGTGGAAACTAGGAGCAGGGAGTTGCTTGGGTGGCCGCTAACACCAGGCT
  ACTCTTATTTTAGCTTGCTAAGTTGAGATCAGCTAGACCTGCTTTCTTTTCTCCTCAGTCTTGCATTTCC
  CTCAATACAAGCTGTAGCCTCTTTCCTCGTTTCTAGTCTCAGAAGGAAGGAGAGGGAAGCCATTCTCCTC
  TAGGGACTCTTCAGTCTCATTTAGATGATAGTCCCTTTTTTTCTACCTCCATATTAGAGATGGAGCTCCT
  TCCTTTTCCTGTTTCTTAATTTTTGTCTTCTCATTCCTGCTTCCCTCTCACCCTATTGCCAGTTCCACCA
  ACTAGAGTGAAAGACTTCCTAGCCATTTCATTAAATCTATTCTGTATCCACCAGGTGGCAGCATCTTGTC
  ATACGTGTCAGGACTTAGGACTGCGGGGTTTAGGTTAGATGTCACGGAAAAAGCTAGTTCTGTGGTCAGG
  CGGCACCAATGAGAAAGGAATGCAGACCCTCCAGATGTATCCTTGGGAAAAGCAGTAAACCAACTAATAT
  TTATTGAAGACCTACTTTGTCCTCTACATAGGGTAGCTTCTGTCAGGGAATCTTGGTTCTTCCCAAGAAA
  CACTGATTTTCTTTCAGGGAGACTTCATGTGTTCATTTATTTCCACCACAGCAGATTTTAAGAAATTATA
  ATATGTAATATTTGATATCTATAAAGAGTATATCTAACGTGAATAAATTATGAAGCATACTAATGAGTAC
  CTATGACCCATAAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000449.3
• Summary: 'A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq, Jul 2008]'
• Locus ID: 5993