Myostatin Propeptide (MSTN) (NM_005259) Human 3' UTR Clone

CAT#: SC215301

3`UTR clone of myostatin (MSTN) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MSTN
• Synonyms: GDF8; MSLHP
• ACCN: NM_005259
• Insert Size: 1583 bp
• Sequence Data:
  Insert Sequence

  >SC215301 3'UTR clone of NM_005259
  The sequence shown below is from the reference sequence of NM_005259. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TTCCAGCGATGGTAGTAGACCGCTGTGGGTGCTCATGAGATTTATATTAAGCGTTCATAACTTCCTAAAA
  CATGGAAGGTTTTCCCCTCAACAATTTTGAAGCTGTGAAATTAAGTACCACAGGCTATAGGCCTAGAGTA
  TGCTACAGTCACTTAAGCATAAGCTACAGTATGTAAACTAAAAGGGGGAATATATGCAATGGTTGGCATT
  TAACCATCCAAACAAATCATACAAGAAAGTTTTATGATTTCCAGAGTTTTTGAGCTAGAAGGAGATCAAA
  TTACATTTATGTTCCTATATATTACAACATCGGCGAGGAAATGAAAGCGATTCTCCTTGAGTTCTGATGA
  ATTAAAGGAGTATGCTTTAAAGTCTATTTCTTTAAAGTTTTGTTTAATATTTACAGAAAAATCCACATAC
  AGTATTGGTAAAATGCAGGATTGTTATATACCATCATTCGAATCATCCTTAAACACTTGAATTTATATTG
  TATGGTAGTATACTTGGTAAGATAAAATTCCACAAAAATAGGGATGGTGCAGCATATGCAATTTCCATTC
  CTATTATAATTGACACAGTACATTAACAATCCATGCCAACGGTGCTAATACGATAGGCTGAATGTCTGAG
  GCTACCAGGTTTATCACATAAAAAACATTCAGTAAAATAGTAAGTTTCTCTTTTCTTCAGGTGCATTTTC
  CTACACCTCCAAATGAGGAATGGATTTTCTTTAATGTAAGAAGAATCATTTTTCTAGAGGTTGGCTTTCA
  ATTCTGTAGCATACTTGGAGAAACTGCATTATCTTAAAAGGCAGTCAAATGGTGTTTGTTTTTATCAAAA
  TGTCAAAATAACATACTTGGAGAAGTATGTAATTTTGTCTTTGGAAAATTACAACACTGCCTTTGCAACA
  CTGCAGTTTTTATGGTAAAATAATAGAAATGATCGACTCTATCAATATTGTATAAAAAGACTGAAACAAT
  GCATTTATATAATATGTATACAATATTGTTTTGTAAATAAGTGTCTCCTTTTTTATTTACTTTGGTATAT
  TTTTACACTAAGGACATTTCAAATTAAGTACTAAGGCACAAAGACATGTCATGCATCACAGAAAAGCAAC
  TACTTATATTTCAGAGCAAATTAGCAGATTAAATAGTGGTCTTAAAACTCCATATGTTAATGATTAGATG
  GTTATATTACAATCATTTTATATTTTTTTACATGATTAACATTCACTTATGGATTCATGATGGCTGTATA
  AAGTGAATTTGAAATTTCAATGGTTTACTGTCATTGTGTTTAAATCTCAACGTTCCATTATTTTAATACT
  TGCAAAAACATTACTAAGTATACCAAAATAATTGACTCTATTATCTGAAATGAAGAATAAACTGATGCTA
  TCTCAACAATAACTGTTACTTTTATTTTATAATTTGATAATGAATATATTTCTGCATTTATTTACTTCTG
  TTTTGTAAATTGGGATTTTGTTAATCAAATTTATTGTACTATGACTAAATGAAATTATTTCTTACATCTA
  ATTTGTAGAAACAGTATAAGTTATATTAAAGTGTTTTCACATT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005259.2
• Summary: 'This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jul 2016]'
• Locus ID: 2660