RAD50 (NM_133482) Human 3' UTR Clone

CAT#: SC215341

3`UTR clone of RAD50 homolog (S. cerevisiae) (RAD50) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RAD50
• Synonyms: hRad50; NBSLD; OTTHUMP00000065928; RAD50 homolog; RAD50 homolog (S. cerevisiae); RAD50-2
• ACCN: NM_133482
• Insert Size: 1554
• Sequence Data:
  Insert Sequence

  >SC215341 3'UTR clone of NM_133482
  The sequence shown below is from the reference sequence of NM_133482. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTGTTAGCTCCCTGGGATTCAATGTTCATTAAAAATATCCAAGATTTAAATGCCATAGAAATGTAGGTCC
  TCAGAAAGTGTATAATAAGAAACTTATTTCTCATATCAACTTAGTCAATAAGAAAATATATTCTTTCAAA
  GGAACATTGTGTCTAGGATTTTGGATGTTGAGAGGTTCTAAAATCATGAAACTTGTTTCACTGAAAATTG
  GACAGATTGCCTGTTTCTGATTTGCTGCTCTTCATCCCATTCCAGGCAGCCTCTGTCAGGCCTTCAGGGT
  TCAGCAGTACAGCCGAGACTCGACTCTGTGCCTCCCTCCCCAGTGCAAATGCATGCTTCTTCTCAAAGCA
  CTGTTGAGAAGGAGATAATTACTGCCTTGAAAATTTATGGTTTTGGTATTTTTTTAAATCATAGTTAAAT
  GTTACCTCTGAATTTACTTCCTTGCATGTGGTTTGAAAAACTGAGTATTAATATCTGAGGATGACCAGAA
  ATGGTGAGATGTATGTTTGGCTCTGCTTTTAACTTTATAAATCCAGTGACCTCTCTCTCTGGGACTTGGT
  TTCCCCAACTAAAATTTGAAGTAGTTGAATGGGGTCTCAAAGTTTGACAGGAACCTTAAGTAATCATCTA
  AGTCAGTACCCACCACCTTCTTCTCCTACATATCCCTTCCAGATGGTCATCCAGACTCAGAGCTCTCTCT
  ACAGAGAGGAAATTCTCCACTGTGCACACCCACCTTTGGAAAGCTCTGACCACTTGAGGCCTGATCTGCC
  CATCGTGAAGAAGCCTGTAACACTCCTCTGCGTCTATCCTGTGTAGCATACTGGCTTCACCATCAATCCT
  GATTCCTCTCTAAGTGGGCATTGCCATGTGGAAGGCAAGCCAGGCTCACTCACAGAGTCAAGGCCTGCTC
  CCTGTAGGGTCCAACCAGACCTGGAAGAACAGGCCTCTCCATTTGCTCTTCAGATGCCACTTCTAAGAAA
  AGCCTAATCACAGTTTTTCCTGGAATTGCCAGCTGACATCTTGAATCCTTCCATTCCACACAGAATGCAA
  CCAAGTCACACGCTTTTGAATTATGCTTTGTAGAGTTTTGTCATTCAGAGTCAGCCAGGACCATACCGGG
  TCTTGATTCAGTCACATGGCATGGTTTTGTGCCATCTGTAGCTATAATGAGCATGTTTGCCTAGACAGCT
  TTTCTCAACTGGGTCCAGAAGAGAATTAAGCCCTAAGGTCCTAAGGCATCTATCTGTGCTAGGTTAAATG
  GTTGGCCCCCAAAGATAGACAGGTCCTGATTTCTAGAACCCGTGACTGTTACTTTATACAGCAAAGGAAA
  CTTTGCAGATGTGATTAAAGCTAAGGACCTTAAGACAGAGTATCCTGGGGGTGGTGGTGGGGTGGGGGGG
  GGTCCTAAATGTAATCACGAGTAAGATTAAGAGCAAATCAATTCTAGTCATATATTAAACATCCACAATA
  ACCAAGATATTTTTATCCCAAGAATGCAAGATTTCAGAAAATGAAAAATCTGTTGATAAATCCATCACTA
  TAATAAAACCGAAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_133482.1
• Summary: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder. [provided by RefSeq, Apr 2010]
• Locus ID: 10111