GRIK2 (NM_021956) Human 3' UTR Clone

CAT#: SC215381

3`UTR clone of glutamate receptor ionotropic kainate 2 (GRIK2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GRIK2
• Synonyms: EAA4; GLR6; GluK2; GLUK6; GLUR6; MRT6
• ACCN: NM_021956
• Insert Size: 1607 bp
• Sequence Data:
  Insert Sequence

  >SC215381 3'UTR clone of NM_021956
  The sequence shown below is from the reference sequence of NM_021956. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACAGAAGGTTGCCAGGTAAAGAAACCATGGCATAAAGCTGGGAGGCCAAACACCCAAGCACAAACTGTC
  GTCTTTTTCCAAACAATTTAGCCAGAATGTTTCCTGTGGAAATATGCAACCTGTGCAAAATAAAATGAGT
  TACCTCATGCCGCTGTGTCTATGAACTAGAGACTCTGTGATCTAAGCAGTTGCAATGATCAGACTTGATT
  TACAAGCATCATGGATCAACCAAGTTACACGGGGTTACACTGTTAATCATGGGTTCCTCCCTTCTTCTGA
  GTGAATGTTAACATGCGCATTTTGTGGCTGATTTCAAATGCAGTCCAGTGAGAAATTACAGGTTCCTTTT
  GAAGCTCAACTGTTGCCAGGAGATGGAATATCAATGCCCAACAGGGCAACCAATAAAAGTGTCACTAAGA
  ATATAAATATTTGGAATCAGCAAAAACTGTAGTGTTACAGGAAACAGTACAGTCTTCTGAACACCCAGAT
  CATAGAGGTGATGATGTTACTAGCCCCCAACTACTCAGTATAATTATTGTCTGAATGCAAAGTATGTGTT
  TATAGGATGTGAAAAAATGTAATGCAAAACAAATTTGAATCCCATGGCAGTTGGAATATAAAGCAGATGT
  TCATCACTTATTTTCCTTTTTTCTTTTCTTATTTTTTTTTTTGACAGTCTGTGTCACTGATTGAGATAGA
  AATGCCAATTATCAAGGAAATAATGTTTTCTTAAGTTCCCTAAGGCAGAAGATTTAACATGCAATTCTAC
  CAGATCCCTTCCTATTCCCCCAACACCTTTTCTCTAACCCCCATATCCCAAATAATAATAATAATAATAA
  TAATAATAATAATAATAATAATAAAAGCAGTTGGTTCAGTGATTCTGAATTAAAAGGATAATGTTTTGCA
  ATGTTCAAGTTGTAAAAACTGGCCGAGTATTGGCTGTGTGGAAGACTAAAGCTTTCATTCTAACATTCAG
  ACATAGCAATCCAAACCCTTGTTCCTGCTGTAAATGAACTTGATGGAGCATGGGCAGATTTCAGTGATAC
  GAGAAAGGGGACTGGTCATCTATAGAAAAATCTGTGAGAGAACTTGGAAGTGGACTGCGTTTATCAATAC
  AGTCACAATGTTAAATGAACAAAATTCTTGAACAGTTTTTTTTCAAAAAATGTTCAGGTTTATTTGTGGA
  AATGCAAGATTTCTATGAAAATAGTTTTTGTATGGAAATTTTTGTAATACTTTTTATCAACAAAACAAGA
  ACATGTGTTCCTGTCAGGGGTGTGATGTCAAGCATGAATGGTAGTGCGTGTGCACCACCAACGTTTGGTG
  AAAACTATTTTTATCAAGAAAAAAGGAATCATAGAAGAGAAATATTTTCAAGTTAGATAATATAAAAGCT
  AGGTGCACTACCACCACTGCTTACCATGCCACACCCCTGGTTTCCACGAGGCTGACAACATACTGTAATG
  AACAATTGTGTGTAAAATGGTAAAAGACACAGACCTCTTGACAACATTGTGATAACAGTTGAGTGCACAC
  AGTTTGCTGTTTGAATCCAATGCACAAAATTAAAAAAAATCATTAAAACTATGTTCATTTTACTTTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_021956.4
• Summary: 'Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive cognitive disability. [provided by RefSeq, Jul 2008]'
• Locus ID: 2898