NRG3 (NM_001010848) Human 3' UTR Clone

CAT#: SC215397

3`UTR clone of neuregulin 3 (NRG3) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NRG3
• Synonyms: HRG3; pro-NRG3
• ACCN: NM_001010848
• Insert Size: 1595
• Sequence Data:
  Insert Sequence

  >SC215397 3'UTR clone of NM_001010848
  The sequence shown below is from the reference sequence of NM_001010848. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACTCTGCATTGACCAAGTGACTTGAGATGTAGGAATCTGTGCATTCTATGCTTTGCTCAACAGGAAAGA
  GAGGAAATCAAATACAAATTATTTATATGCATTAATTTAAGAGCATCTACTTAGAAGAAACCAAATAGTC
  TATCGCCCTCATATCATAGTGTTTTTTAACAAAATATTTTTTTAAGGGAAAGAAATGTTTCAGGAGGGAT
  AAAGCTTACCATTAAAGCTTTTGGGTAGAATTCTGAATCCAATTTCGTTTAGTCCCAACACTGTCCTCTT
  TGGCTCTTAGAAGATGTGGGCAATTCAGACCCTTGGCCCCACAGTGCCAGTGTCTCATTAAAAAACACTG
  GCAGTTACCTGGTTTCAAAAAGAGGAGAGCTGCATCCCGCAGGTGGATGCACCAGTGAGCAGGTGGCTCT
  TGCCATTTGGCTTGCCAGTCCCAAGCCCTAAATTTCTATTCACCCAATAGCCTCATCACAGGTTATGTCA
  TGTCGACACATGTAGTGTTTTCTATTTGATTTTTGGAGAATGCCACAAAAGACTCTGCAATGGGAAGGAG
  GACAGGAGATAGAAGGAGAAACCGGGGGCAATCTTAACTACTCTTGTGTGCCACCTTCCTCTGAGATTTG
  AATGCACAGATCTCAGCTGGAGGTATGAAATTATTACAGAAAAAGAGACAAAAAAATCCTTATATCGTGT
  CACTAAAATCATGCTAATAGAAATGTTTTTCCTTGAGATTGTTTAGAGGCTCTGGAGGAGCGTTTCTCAG
  TTTTTGTGTGCCTGTGTGCACATGTGTGTGCGAGCGTGTGTGTGTGTGGACTTGCTCACGCGGGCACATA
  TGCTGTAAGCACATGTGTTCATTGTGCGTATGTGTGTGCATGTGTGCGCGTATTACGCTTGCTAAAATTT
  GTTCTGAAACATCAGGGAATCTACTATTCAGAAAAATTATTTCCCTCTTAGATCTTTTCACTTTAAATTT
  TTCCATTAAGTATAAAGTTCAGTTAGTTCTTAAATCAAGGTCACTTGCATGGTGGGGTCGTATAAAACTC
  TTGACACTGTCTAGACCATTTTCTGATGTGAATACTTTTGAGAGGATCAACTATTGGCTCATTAATGATA
  TCAGTATCATAAGGTGAGTTCATCAAGGATGTGTGTTTATTTTGAATCATGCCTACTTAAATTATTAATA
  CAAGACAATTAACAAATTGACAGTTACCATTTGCTTTCTCATCATCCTCATTACCATTTATTTTATAGCA
  AGTCTGCTATGTGTGGACCAAGGCTTCGGCTTCTGTGGTTAGTATGGGAAGAATAAATTGTTGAAATAAA
  AATACCCAGACTATTCAGTTCACAAGAAGCCCCCCAAAAGAACAGTAAATTGTGTTGTATGTTCATTTGG
  AATAAAGCAATATTTTTACCACTGCTAAGGTGAACTGAAACCTCTCCTGAAGATTTGTCTGTTTATTTAC
  CCTCACTTTCATGGGACATATGAGGAAATTAGTGTTCACATATCCAGTCTTTTTCCAATTATTGGTGGTG
  TTGAGTTGTTATGTTTACACTGTTTTAAATAAAAAGGCACCGTATTTGAAAGCAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001010848.3
• Summary: This gene is a member of the neuregulin gene family. This gene family encodes ligands for the transmembrane tyrosine kinase receptors ERBB3 and ERBB4 - members of the epidermal growth factor receptor family. Ligand binding activates intracellular signaling cascades and the induction of cellular responses including proliferation, migration, differentiation, and survival or apoptosis. This gene encodes neuregulin 3 (NRG3). NRG3 has been shown to activate the tyrosine phosphorylation of its cognate receptor, ERBB4, and is thought to influence neuroblast proliferation, migration and differentiation by signalling through ERBB4. NRG3 also promotes mammary differentiation during embryogenesis. Linkage studies have implicated this gene as a susceptibility locus for schizophrenia and schizoaffective disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but their biological validity has not been verified. [provided by RefSeq, Sep 2009]
• Locus ID: 10718