HERC3 (NM_014606) Human 3' UTR Clone

CAT#: SC215405

3`UTR clone of hect domain and RLD 3 (HERC3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HERC3
• Synonyms: KIAA0032
• ACCN: NM_014606
• Insert Size: 1581
• Sequence Data:
  Insert Sequence

  >SC215405 3'UTR clone of NM_014606
  The sequence shown below is from the reference sequence of NM_014606. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTTGACAACTATGAAGGGTTTAGTTTGGCCTGAGGCTTCTCAGCTTGTCCAGTATTTCCCTTCGTTCCTC
  AGTGTCCACATTGAGGCCTATACAGAAAATCATGGGGAGTGATTTCTATTTTTTTATTGTCTAAGTGGGT
  TGGGACTTTTAAATACTGAGCCTGGTTGATGTGTTTCTGGGATTGTATAGCAGTAAACAACCTTTTTGAA
  AAATTAGAGGTTGGGGATGGGGTGAAAAATTGGCCCTTGTATGGGAGGTGTTTTTGTTTTTGTTTTAAAC
  CAAACTACCCAGTATTCCTTGCACTTGTGAATGTGTTGCACTCTGCTGGATGAAATGGCAGTGGATTTTT
  AAACTTTAATTTCCCAAATGTCTCTCTCAGCCCTGATGTTTTCTCACAGTGCTTCCTTGTCCTTCTCTTA
  ACTTCTCATTCCTCTATAAGAATGATTTAGACTGACCTGTCCTTTTTTATCTGCGCATGCGAGAACATCA
  CCTTCCTCTGTACACTTGGAAATGCCTCTGGCTTGTTGCAGCCCTCCTTTAACCCAAAGGAGGAAAGGAC
  TGCTTCAGAAACTCCCAATTCCAAAAAGCTGAGTCTGGGTCCATTATTTTGGCAGAACTCCTAAGAATTT
  ATGGGAGCCTATATAAACATATCTTGCTTTTAAAAAGTTCTTGAGGGAATAGCAACTTTCCCATGGCTGT
  GCCTATTTCCTAGACCTTTTAAAAGATGTGCAGAGCAGCTTAGCATTCGTTGCAGCTGAGCCTAATTTTT
  TCTTGCTCATCCTTGTCCCTTTGACAATAAGGTTAATTGATAGACCCACCACCTCTTGCACTCTCGCTTT
  TGGAGCAAGTTGCATTAACTATTTTGAGTCTCTATATTGTCCAAGAAAAGTAGAAATAATAAATTTACTT
  TCCCTTTTTCTATCACCTTATGTCCTCTACCATTTTCTCCTTCCTCCCTTCCCTTATTTTCTCCTTTTCG
  TACCCTGTGTCCTCCCTGATTTTCCTTTCGTTTCTTCTTTATTTTATCCCATTCTCTGTTACTTGACTCA
  GTGCTCCCTTCCTCTCCTCTCCTTCTAGTGGATGCATGCAGCCTTTTTTTCAATTTTTATTTAAATTGCA
  AAATTTTTACTCAGATTTTTTTTCCTCTTCCCTAATTGCTAAGATTTAAGGACGTTCTTTATTATGAAAC
  TTTATCACATTCGAAATGTTTGTTTACAGTGGGATTTTAGGGGGGATTGTGTTTAAATCAAATATATGTA
  TTTTAAAAATAATGACATGCTCAACCTTCCTCATCATGGAGTAAGAAAATTCTACATGATTAAAGAATCC
  ATGTAAGTCTAATTTTAAATTCCTAGTAACTAGAGAAAAGACTTATTTATATAAAATGAAGTATTTATGA
  ACTGTGATAAAGCATCAAATCTTGATGAAGGATTGTAGATTTTTGCTTTTTCTTTTTGTTTTTAAAACTT
  ATTCCAATTGCTAAATTGGTAGTTTTTCAGTCTTTATAAATACAGGATTAAAAATATATATACAGTTATA
  TGAAATGTTTATTTTCTATGTGTGTGCATATAGTTCAATAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_014606.1
• Summary: This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012]
• Locus ID: 8916