SNAP23 (NM_003825) Human 3' UTR Clone

CAT#: SC215436

3`UTR clone of synaptosomal-associated protein 23kDa (SNAP23) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SNAP23
• Synonyms: HsT17016; SNAP-23; SNAP23A; SNAP23B
• ACCN: NM_003825
• Insert Size: 1589
• Sequence Data:
  Insert Sequence

  >SC215436 3'UTR clone of NM_003825
  The sequence shown below is from the reference sequence of NM_003825. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCAATGCCAGAGCAAAGAAACTCATTGACAGCTAAAGCTACTGCTGTTCTTCTTTATCATTTATTCACT
  TCCGTAGCTCCTCCTTGAAAGTTATTACCTTTTCAGAGTTTAAGTTTTCGGTTCCACGCTCTTCTAATTG
  GGAGATAATATGGAAGAAGGGCCAGAGCAGTTACAGCCCTCCTTCTTTTTTGTTTTCTGTTGAGGGCCGA
  CTGCTGCTCTGCCTTCCTTCTAGTATTTTCTTTCTCAATTCATACGCTTAGATTGGTTTTCATATGTCAT
  GTTTAGTGTTTTCATCCTCCTCATATACTTCAGCAGGTTCTTTTGCTTTCAAGATTTGGAAGCATTGCCA
  AAGACAGCCATGAAGAAGGAAGCTGTAGAGGTGTTTTTTGTTGTTGTTTATTTTTGCTTTTGTGGTTGAG
  GGAAGGACAAGAGATAAGAGGTTGTTACCTCAGTAAAAACCTTCAGGCCACAAAGCAAAAAGTTGCATAG
  CCACAACGAAGATCTAGTTGGATATAGTTTTTGATTTAAGTTGCAGTTATAGCCAATTTAGGCTAATGCT
  TGGTTTTGGAGCTTTTATACACAACGTTTTTGTTAGGCATCACAGTTTTGCAACCTCTGCTCCAAAGAGA
  AAAATAGAATGAGTTTTCTTTCTTTTTTTTTTTTTTGGAGTCAGAGTCTCGCTCTCTTGTCCAGGCTGGA
  GTGCAATAGCGCGATCTGGGCTCACTGCAACCTCCGCCTCCCTGGTTCAAGCAATTCTCCTGCCTTGCCT
  CCTGAGTAGCTGGGATTACAGGCGTGTGTCACCACGCTCGGCTAATTTTTGTATTTTTAGTAGAGACAGG
  GTTTCACCATGTCAAGCTGGTCTTGGACTCCTGACGTCGTGATCCACCCGCCTTGGCCTCCCAAAGTACT
  GGGATTACAGGCGTGAGCCACTGTGCCTGGCTGAGTTTTCTTAAAGTGAGCTTAATTTCTGAACTCTTAG
  TGATTCATATATGTACATAAATCTGTGATCCCATTTCTTATTGCACCATTCAGGAACACTTTATATAAAT
  GAGTGGCTTTTTATTTCATATTATTAGTAGTATCATGGTTCCATTACAGGCCTATTAACATCATACATTG
  TCATTAGTCTTTGAAGAAAAAATATGTAAATATATATGTGTAACATGAGAATTTCTCTCTAAAGCAGGGC
  TTAAAATTTTTTGGAAAAGTTTGACAAAGCATACCACATGAATTCAGATTTACCTCAATGCTAAGAATTA
  TGTTTAGTTAGGAAAAAGGAAAGTCATTTTGACCTCAGGTAGAAAAATAGATTGCTTTGAGTTTTATGTA
  GCTTTAGACTTTAAAAAGTTAGAATTTATTCTGTAACTAAAAATTATTTGAAAAAATTATGCCTCTGGTT
  TAATTATTGGTGATTACACACTCTTTCTCTTACCCTTGTGTATTGAACTATGTCCATAATCAAGTTGATG
  TGGATCCTGAAAAGTGTTATGAACATCTGATTGGTATTTGTCACATTTATTTTAAAATTAGCATCTGAAC
  ACTTCAAAGCTGTCAGTGTGTATTGGTTTCACCAATAACCACTGCTTGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_003825.2
• Summary: Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 8773