RAD21 (NM_006265) Human 3' UTR Clone

CAT#: SC215489

3`UTR clone of RAD21 homolog (S. pombe) (RAD21) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RAD21
• Synonyms: CDLS4; hHR21; HR21; HRAD21; MCD1; MGS; NXP1; SCC1
• ACCN: NM_006265
• Insert Size: 1594 bp
• Sequence Data:
  Insert Sequence

  >SC215489 3'UTR clone of NM_006265
  The sequence shown below is from the reference sequence of NM_006265. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATCGCAACACCTGGACCAAGGTTCCATATTATATAAGGAGCTAGAAGCATTATAGCTAGTGTTTGATTCA
  CTAGTGCTTACAAATTGCCCCCATGTGTAGGGGACACAGAACCCTTTGAGAAAACTTAGATTTTTGTCTG
  TACAAAGTCTTTGCCTTTTTCCTTCTTCATTTTTTTCCAGTACATTAAATTTGTCAATTTCATCTTTGAG
  GGAAACTGATTAGATGGGTTGTGTTTGTGTTCTGATGGAGAAAACAGCACCCCAAGGACTCAGAAGATGA
  TTTTAACAGTTCAGAACAGATGTGTGCAATATTGGTGCATGTAATAATGTTGAGTGGCAGTCAAAAGTCA
  TGATTTTTATCTTAGTTCTTCATTACTGCATTGAAAAGGAAAACCTGTCTGAGAAAATGCCTGACAGTTT
  AATTTAAAACTATGGTGTAAGTCTTTGACAAGAAAAAAAAACAAACAAACACTTCTTTCCATCAGTAACA
  CTGGCAATCTTCCTGTTAACCACTCTCCTTAGGGATGGTATCTGAAACAACAATGGTCACCCTCTTGAGA
  TTCGTTTTAAGTGTAATTCCATAATGAGCAGAGGTGTACGCGAAATTGTGTTATGACTGATAGCCTTCAG
  CTACAAAAAGATAGGACTGACCTGGTTTAAAGTGTTCTATTTTGTAAATCATTCCATTTGAGTCTTTCTG
  ATGAACTTGGCTATACTGAAATCTGTTATTTTAGTGAGGCTCCAAAATGAGCAAAGCTAGGCCTGATTAG
  AGTAGAGTGACTATTAAAAAACATAACTTTCTAGGAGCTATAAATCAAAGTTTTAAAAAGATGTTTGGAT
  ATATTTGAGTATTCCGATCATGAAAACAGAAATTGCCCTGCCTACTACAAGGACAGACTGATGGGAAATT
  ATGCACCTGGTCAACTTAGCTTTTAAGCAGACGATGCTGTAAAAACTAACGGCTTCTCTGATATTTATTG
  TAAGTTTTAGTACTGATCTCCTTTTCCAGTGCTGCACACTCCTGGTTTGGAACTTTAATAGCGTTGCAAC
  GAAATCCTATATCCAGTTTCCTGTAATTTAATTGAAGAAAAATACATCCAAATAAAGACTTTATTATTAA
  CAGACCAGATAGCATCAGAAATCATGTGACTGTTATGATTATCAGAATGTCTTAACTTTTTAGGGCAAAG
  TTAACACTGAAAGTTCTAGCTTAAGTGTTGAAACTTTTGTGGGAAAAAAAAATCACTTTTGAAACTCAGA
  CTTCAGTGTATACCCAATAATTTAAAATTATGTGAAATGTTTTAAATTTGTGAACTCGTAATTACTGTTT
  TAATGATTCAGTTTCTTCAGAGTGGTAATTGTATAAAATTGCTATTGCAGCTTTACATTCAATATGATGT
  GCCTGTAAACCAAGGAGTTTTCCCCGTTTGTAAAAAGACATTGTAGATAATTGAATGTTTGATTTTAGAA
  AGGTCATTAGTTTCTTGTTACACATTTTGTTAGTCTGGTTTTTGTTGCTTATCGGGTTTAATATTGTTCT
  TGAAAATAGTTGATGCTATGTTATGTATAACTTTTCTAATAAAAGTTGTGTTAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006265.2
• Summary: 'The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]'
• Locus ID: 5885