Caspase 3 (CASP3) (NM_004346) Human 3' UTR Clone

CAT#: SC215502

3`UTR clone of caspase 3 apoptosis-related cysteine peptidase (CASP3) transcript variant alpha for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CASP3
• Synonyms: CPP32; CPP32B; SCA-1
• ACCN: NM_004346
• Insert Size: 1610 bp
• Sequence Data:
  Insert Sequence

  >SC215502 3'UTR clone of NM_004346
  The sequence shown below is from the reference sequence of NM_004346. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCCATGCTCACAAAAGAACTCTATTTTTATCACTAAAGAAATGGTTGGTTGGTGGTTTTTTTTAGTTTGT
  ATGCCAAGTGAGAAGATGGTATATTTGGTACTGTATTTCCCTCTCATTTTGACCTACTCTCATGCTGCAG
  AGGGTACTTTAAGACATACTCCTTCCATCAAATAGAACCACTATGAAGCTACCTCAAACTTCCAGTCAGG
  TAGTTGCAATTGAATTAAATTAGGAATAAATAAAAATGGATACTGGTGCAGTCATTATGAGAGGCAATGA
  TTGTTAATTTACAGCTTTCATGATTAGCAAGTTACAGTGATGCTGTGCTATGAATTTTCAAGTAATTGTG
  AAAAAGTTAAACATTGAAGTAATGAATTTTTATGATATTCCCCCCACTTAAGACTGTGTATTCTAGTTTT
  GTCAAACTGTAGAAATGATGATGTGGAAGAACTTAGGCATCTGTGGGCATGGTCAAAGGCTCAAACCTTT
  ATTTTAGAATTGATATACACGGATGACTTAACTGCATTTTTAGACCATTTATCTGGGATTATGGTTTTGT
  GATGTTTGTCCTGAACACTTTTGTTGTAAAAAAATAATAATAATGTTTAATATTGAGAAAGAAACTAATA
  TTTTATGTGAGAGAAAGTGTGAGCAAACTAACTTGACTTTTAAGGCTAAAACTTAACATTCATAGAGGGG
  TGGAGTTTTAACTGTAAGGTGCTACAATGCCCCTGGATCTACCAGCATAAATATCTTCTGATTTGTCCCT
  ATGCATATCAGTTGAGCTTCATATACCAGCAATATATCTGAAGAGCTATTATATAAAAACCCCAAACTGT
  TGATTATTAGCCAGGTAATGTGAATAAATTCTATAGGAACATATGAAAATACAACTTAAATAATAAACAG
  TGGAATATAAGGAAAGCAATAAATGAATGGGCTGAGCTGCCTGTAACTTGAGAGTAGATGGTTTGAGCCT
  GAGCAGAGACATGACTCAGCCTGTTCCATGAAGGCAGAGCCATGGACCACGCAGGAAGGGCCTACAGCCC
  ATTTCTCCATACGCACTGGTATGTGTGGATGATGCTGCCAGGGCGCCATCGCCAAGTAAGAAAGTGAAGC
  AAATCAGAAACTTGTGAAGTGGAAATGTTCTAAAGGTGGTGAGGCAATAAAAATCATAGTACTCTTTGTA
  GCAAAATTCTTAAGTATGTTATTTTCTGTTGAAGTTTACAATCAAAGGAAAATAGTAATGTTTTATACTG
  TTTACTGAAAGAAAAAGACCTATGAGCACATAGGACTCTAGACGGCATCCAGCCGGAGGCCAGAGCTGAG
  CCCTCAGCCCGGGAGGCAGGCTCCAGGCCTCAGCAGGTGCGGAGCCGTCACTGCACCAAGTCTCACTGGC
  TGTCAGTATGACATTTCACGGGAGATTTCTTGTTGCTCAAAAAATGAGCTCGCATTTGTCAATGACAGTT
  TCTTTTTTCTTACTAGACCTGTAACTTTTGTAAATACACATAGCATGTAATGGTATCTTAAAGTGTGTTT
  CTATGTGACAATTTTGTACAAATTTGTTATTTTCCATTTTTATTTCAAAATATACATTCAAACTTAAAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004346.3
• Summary: 'The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017]'
• Locus ID: 836