cGKI (PRKG1) (NM_006258) Human 3' UTR Clone

CAT#: SC215614

3`UTR clone of protein kinase cGMP-dependent type I (PRKG1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PRKG1
• Synonyms: AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha; cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B
• ACCN: NM_006258
• Insert Size: 1651 bp
• Sequence Data:
  Insert Sequence

  >SC215614 3'UTR clone of NM_006258
  The sequence shown below is from the reference sequence of NM_006258. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGAACCACCACCTGATGACAACTCAGGATGGGATATAGACTTCTAATGTATTTCTCTTACCTGCTTCTGC
  CTTGCTGAAGACAGCTTTTTCTGAGACACAGCTGCCAGCAAACCTGAGGGAAAGAGAGAAGATTAGTGCT
  CGGGGTCACCATGATGCCTTTGATCGATGCTGCTCCAGTAACTACAGTGGCATTAGGACTTACCGCTTAG
  ATGACAATAGTGCTCTTTACATGTTTTCTGTTTGAACCTAAAATAGCAGTTGACATGGTGGTCCTGAAGC
  AAAGCCTTTCACCAGTAAAAGATGTTTTCTATTGTTGCAATGACCTTGCTTTGCTCTGATTATAATTTGA
  AAGACTGTAGGAAACACTTCAATGTAGTATAAGAGTCTGTACCTTGCTGGAATATTCAAGAAGATGAAAG
  AATAATATATTGGGTACAATAGATTACTATGGTACAGAAACTGGGCTATTCCCTTTCTTCAAGTGAAGGC
  TGTGGGATCTATTACTGCAGGCCGGTGTATATACCATACAAAAGAGGACCACACATCTGTTGGTCACAGA
  GTTCATGTCACACCAGTGCTAGAAGTTTCATGATTTTATTTCCCAGCAGTGCTGATGACAAGACTGAATG
  TTACCTTTTCTTTCTGACAGATTTTAAAAATTGATATGATAAAAGCACAACTGCTATAGATTCTGCTGAG
  ACCTCTCATAGTAGGTATATATGAGTTTTCACAGAAGACTGAAAAATAATGCATGATATTTGTTTGTTTT
  TTTTGATAAATTGGCATGACAGAGTGGGGAAAAAAAGCAATTCACAAAACCATTTCATATTTTTTAAAAT
  ATTGTGCTTAAAGATGGTCCTGGAAGTAAATGACTAGCAGCCAATTGGTTTTACTTAACATACCCTCAAA
  CTGAGGCTTAAAGTATTCCCTTTTATAAAAATAAATGCTTGGGGTAGGGTGGAGTGGGGAGGGATTAAAA
  CCCATCCAAAAAATAAATAAAAACTATATAGGTGCTATGTATATCTTTCATCTGTAAATGTCAGTGTCTG
  AACAGACAACACAAATTCAAATCATTATACGTGTAGCCAGAAACTCAAGCATTTTCACTAAAGTTATTAA
  ACCAAACTCCTGTCCAATTTCACTTATACAACATAGTCAGTCTAGAGTTGAGAGACAAAGGTAATTATAA
  ACCTATTTGAACTAGCTTCTTGTCTTAGGCCTGAACCAAAAAACAACAAACAAACAAAAAACAAGAATGA
  AAAACAGAAATAAAAGAAGTAGAAAAGACAAAGAAAGAAAGCCCAAAGTCAAAGTTGTTAATATTTACAG
  GTTTACCAGATCTGGAACATTACTTATTTGAGGTCAGAGAACAAAACAAGAACCTGGCCAGGTGTTGATT
  ACCTTTTAGTGAATAAGCTGAGTCCATATACTTGTCTAACTAAGAAAGCAGTACAGAGGAAAACAGGAAC
  CTGATTTTTTTAAAATAAATTTTAAATAAAATAGAATTACTACAATTCTGCAATTTCATACTACCTAAAA
  AAGACTAGATTTGAAAATGTCAAGCTGATTTACTTTATTCACATGGAGAAAAGAATCCACAAATTAAACT
  GAGTCCTTCACTGGCATGCCAGTTGACTATTATTAGCTGTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006258.2
• Summary: 'Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]'
• Locus ID: 5592