alpha Adaptin (AP2A2) (NM_012305) Human 3' UTR Clone

CAT#: SC215628

3`UTR clone of adaptor-related protein complex 2 alpha 2 subunit (AP2A2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AP2A2
• Synonyms: ADTAB; CLAPA2; HIP-9; HIP9; HYPJ
• ACCN: NM_012305
• Insert Size: 1619 bp
• Sequence Data:
  Insert Sequence

  >SC215628 3'UTR clone of NM_012305
  The sequence shown below is from the reference sequence of NM_012305. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGTGAATTGCTCTCAGCGCAGTTTTAGTCCTGAGGATGGAAGACCAGGCTCGTGTGTCTTGTGTTGTCTT
  CGTCTGTGCCGTTTGTCTTCGTGGCCATCCTGCAGATGAGCACCGTGTCCAGTGCCACAGCACAAGGCGC
  CTCCCCGCCCCGCCGCCCCACACCTCTCCCCTTTGGGCTGGACGGGAACACACGTGTGTGGCTCAGGAGG
  AAAAGCTCAGCCTGGACTGTGGCAGCCACGGCAGAAGGTGGATCTTGGGATCAATTTTTATAAAAATCGA
  GACAGTTCTGTGGTTAAATCTACAAATTAAAGGGAAATTAGAAGTTGGCGTGAACGTGGCGTTTGTGGGA
  GTGTCACTGAGATGGCCCGTGCTGCCGCCCACCCCGCCTCGGAGCCTCTGGGAGCAGCAGTGCCACTGTG
  CATGGCGTGGGCTGAGCCTTGGTGTGTGGCCGTCCTGGTGGCTGCACACCTGGCGTCGTCCTGGGCCCTT
  GGGAGGAGCACAGCTGACCCTGGTTTTGCTGCAGTCCCAGCTGGACTGTTTTCCCAGGCAGGATTTTAAT
  CTAGAATTTAGAAACATTTGTATTTGTAATGACTTCTGGCAAAAGCACGTGTCCTGGCCGGATGTAACTG
  TTCTCCTTTCCCAGCTCCTGTTTGTGAAGGGCGTCTGTTATGCTCCTGCAGTCGCCGAGGCCTTGGATGT
  GCAGCCAGGGGAGGAGCGTCCTGCCGGCCCCGCAGGGCCCCCAGGACTCCAGGGTAAAGTGTGGGCCGGT
  GGCGCAAGACTCAGAGGTGTGCTCGTCTCTTTCCTGTCAGAGTGGGCGTCCCCAGGCCACGGTGCAGGCC
  TGAGTCCTTCCACCGGCCCCGTCCAGTCGTCCCTGGAGGGGCTGTGGAGGAGGGACGCCTCTGTGTGGTC
  AGGAAGTGAAGGGGCCATTGGCCGCATGCCATGTGCCACCTGCGGCTTGTGTCTCACCTGTCATCTGGAC
  TCAGCACCCAGGCTGCACGTCTGACACCTGAGAGGCGAGAGAGTGGGGCCGGCCTAGGAGCCAAGGCTGG
  GGCCTTGCGCTCTGTCCCCAGGATGGTGGCCTTGTTTGTCCTAAACACACCCAGCACAGGTTCTGGCTTC
  CTGACATGCTGTGGAGGCAGGGAGGGTGGGTGGCCACATGTGCTTGAGGGTTTTCACCCTGGCCCTCAGT
  TGCCTGCTGTGCGGGTCCCTGGGGCAGCTGCAGGGGCTCATGGACCCATCAGGGTCTCCACAGCTCCCCT
  GCAGTGTGTGCACCCCACAATGTCTGCGGCTCTTCTTCCGGCGTGTCGGGCTTTGATCACAGCATAGCCA
  CGTCAGTGGCGTGCGCCTCTCGCACAGGCCATTCTGGGTCTGGTGGTGCCAGGTGCCGTGACACGCCGTG
  CTGGGCTTGTGCTGCAGCTGGGTGGTGTGGCCCTCATTCTCATGTTCCAGCTGCTGGGCAGTGCTCTGCC
  TGTGTGCTGCGCCTGCAGGCTGCGTGTGCTGCCGTGGATCTCCTGCATCCCTTGACCCCTCCCGCCATCA
  GAGGAAAGGCTGCTCCCCGAGGCACCGCTTCCCTGTGCGGCGCTGCAGAGGGGCCCTCAGTGTGGCACTC
  CTCGTCAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_012305.2
• Summary: 'The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane. [provided by RefSeq, Nov 2016]'
• Locus ID: 161