VE Cadherin (CDH5) (NM_001795) Human 3' UTR Clone

CAT#: SC215693

3`UTR clone of cadherin 5 type 2 (vascular endothelium) (CDH5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CDH5
• Synonyms: 7B4; CD144
• ACCN: NM_001795
• Insert Size: 1663 bp
• Sequence Data:
  Insert Sequence

  >SC215693 3'UTR clone of NM_001795
  The sequence shown below is from the reference sequence of NM_001795. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGCTGAGCTGTACGGCTCGGACCCCCGGGAGGAGCTGCTGTATTAGGCGGCCGAGGTCACTCTGGGCCT
  GGGGACCCAAACCCCCTGCAGCCCAGGCCAGTCAGACGCCAGGCACCACAGCCTCCAAAAATGGCAGTGA
  CTCCCCAGCCCAGCACCCCTTCCTCGTGGGTCCCAGAGACCTCATCAGCCTTGGGATAGCAAACTCCAGG
  TTCCTGAAATATCCAGGAATATATGTCAGTGATGACTATTCTCAAATGCTGGCAAATCCAGGCTGGTGTT
  CTGTCTGGGCTCAGACATCCACATAACCCTGTCACCCACAGACCGCCGTCTAACTCAAAGACTTCCTCTG
  GCTCCCCAAGGCTGCAAAGCAAAACAGACTGTGTTTAACTGCTGCAGGGTCTTTTTCTAGGGTCCCTGAA
  CGCCCTGGTAAGGCTGGTGAGGTCCTGGTGCCTATCTGCCTGGAGGCAAAGGCCTGGACAGCTTGACTTG
  TGGGGCAGGATTCTCTGCAGCCCATTCCCAAGGGAGACTGACCATCATGCCCTCTCTCGGGAGCCCTAGC
  CCTGCTCCAACTCCATACTCCACTCCAAGTGCCCCACCACTCCCCAACCCCTCTCCAGGCCTGTCAAGAG
  GGAGGAAGGGGCCCCATGGCAGCTCCTGACCTTGGGTCCTGAAGTGACCTCACTGGCCTGCCATGCCAGT
  AACTGTGCTGTACTGAGCACTGAACCACATTCAGGGAAATGGCTTATTAAACTTTGAAGCAACTGTGAAT
  TCATTCTGGAGGGGCAGTGGAGATCAGGAGTGACAGATCACAGGGTGAGGGCCACCTCCACACCCACCCC
  CTCTGGAGAAGGCCTGGAAGAGCTGAGACCTTGCTTTGAGACTCCTCAGCACCCCTCCAGTTTTGCCTGA
  GAAGGGGCAGATGTTCCCGGAGCAGAAGACGTCTCCCCTTCTCTGCCTCACCTGGTCGCCAATCCATGCT
  CTCTTTCTTTTCTCTGTCTACTCCTTATCCCTTGGTTTAGAGGAACCCAAGATGTGGCCTTTAGCAAAAC
  TGGACAATGTCCAAACCCACTCATGACTGCATGACGGAGCCGAGCCATGTGTCTTTACACCTCGCTGTTG
  TCACATCTCAGGGAACTGACCCTCAGGCACACCTTGCAGAAGGCAAGGCCCTGCCCTGCCCAACCTCTGT
  GGTCACCCATGCATCTTCCACTGGAACGTTTCACTGCAAACACACCTTGGAGAAGTGGCATCAGTCAACA
  GAGAGGGGCAGGGAAGGAGACACCAAGCTCACCCTTCGTCATGGACCGAGGTTCCCACTCTGGGCAAAGC
  CCCTCACACTGCAAGGGATTGTAGATAACACTGACTTGTTTGTTTTAACCAATAACTAGCTTCTTATAAT
  GATTTTTTTACTAATGATACTTACAAGTTTCTAGCTCTCACAGACATATAGAATAAGGGTTTTTGCATAA
  TAAGCAGGTTGTTATTTAGGTTAACAATATTAATTCAGGTTTTTTAGTTGGAAAAACAATTCCTGTAACC
  TTCTATTTTCTATAATTGTAGTAATTGCTCTACAGATAATGTCTATATATTGGCCAAACTGGTGCATGAC
  AAGTACTGTATTTTTTTATACCTAAATAAAGAAAAATCTTTAGCCTGGGCAAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001795.3
• Summary: 'This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015]'
• Locus ID: 1003