ACTN2 (NM_001103) Human 3' UTR Clone

CAT#: SC215765

3`UTR clone of actinin alpha 2 (ACTN2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ACTN2
• Synonyms: CMD1AA; CMH23
• ACCN: NM_001103
• Insert Size: 1659 bp
• Sequence Data:
  Insert Sequence

  >SC215765 3'UTR clone of NM_001103
  The sequence shown below is from the reference sequence of NM_001103. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATTACGCTGCGTTCTCTTCCGCACTCTACGGGGAGAGCGATCTGTGATGCTGAGCTTCTGTAATCACTCA
  TCCCATCAGAATGCAATAAAAGCGGAAGTCACAGTTTGTTTCCTGGAAACTTTGACAAGCTTTATTAAGT
  TGAGAGAGAGAGAGGGGAAAAAAAAAAGCCTTTCGTAGTTCAGTAATTGCCAGCAATATAACACGGCTAA
  AATGAAGTTTTTACAGTATATGACATAGTGCGCTTCATAAATAGGTTTATTTCTGAGTTTTTAGCAAAAT
  GTAATGAAATATCAGGTTGATTTCTTTGATTAAACAGAACAAATTACTTGAGTAATAGGAAATTAGGAGG
  ATCTAGGGACAGAAGGAAAGTGAAAAATGTGAAAATACAAAATACCCAAGATTTAAGACCGGGGGGAAAA
  AACCACAAATTGGTAAATAAAGGTTTGCTATTTGTAAAAAATTTCATTTATCTCTAATATGCTTATGTGA
  TTGCCCCTAGGGGAGTATATTTGGGATTCTAATGTTTTATTTTCATGCTTATCCAAAGATTACTATTGTA
  TCTTCAAATGAATTTAATATTGTGAGATGGAACTGCCGGGGATTAAAAAGACTACCCAAAAGATTTTTGG
  CACTTACAATTTTTAAAATAGTTTATGTCATCTCTTCATTATTTAGGGCTGGATGGTCAACTCAGTCAGT
  GATTTTTTGATGCTTCTCTTATCCTCCAGAATAGAGACCTAAGGACACGTGGAAGTCAGTTTAATTGCCA
  GAGAGAAGGATGCAATCACTAGGTAAAATGAGGTTTTTAGGATTATTTATTGATTCCAGGTTCCCATGCT
  TTTTGTTAGAGCTTATTAGTACAGGTTCTCAAGAGATGACCACATAAAAGTGCTCTGTTTATAAATAAGC
  AGGTTTCTGTAGTACTGACTGGTTCATCACAAGGCAAGTCAGAAACCAGTATCCTTCTAGCTCTCCAGTC
  AGGACTTCCTTATGCCTCTAGTTTTATGACCGGTTAAGGAGAAGCCAGAGTTAGAGTAGGAGAGGACTAA
  TTCTCAGCAGCAGTGGAGGTGAGTTCTTTCTTTTGCGGAAGCTTTACATATGTTTTGTGTAGTAGGAATA
  ACTAGATATTTTAGCTAGTGTGCGGTGTGTGTTCACCCCTGGGATTGGACAGTGTATCCTAACAAGTCCC
  ATGTCTGGTTCTGTGTCTAAAGGCCTGCTCCATGACACAGGATGCTACATGCACTCCTGCTAGCACATCT
  TGATCTGTTGAATGTTCATTCTTTCTTTTTGCTCATACTGCTGTAGGCTATAATTCCCCCCTGTTTTTCC
  ATCTTGTTGACAGCTTGTAGAGAATAAAGCAGGAATTCTTTTTATATCTGAGTCCTTAATGATCAGGAAA
  TGGATTACAAACAAACAAAAACAGTTGCAAACAGCAATTAAAGCATTACTAGAGAAGCAGTAACATGTCT
  GTTACCTACAGTCTGTTTGCTCAGATTTTTATAGATTTCCACCAACAGCTGGAAATGTAATATTTCCATT
  CCAAGCTGTCTGACAGCTTGACTCTTCTTCCTACACTGGGCCATTTAGAACCTTCAACCTTTATGAATTT
  CACTTAATTTGCTTCATTATTAAGTAATTGGTAGTTTCCAGATTCCTGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001103.2
• Summary: 'Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]'
• Locus ID: 88