UBE2D2 (NM_181838) Human 3' UTR Clone

CAT#: SC215794

3`UTR clone of ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog yeast) (UBE2D2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UBE2D2
• Synonyms: E2(17)KB2; PUBC1; UBC4; UBC4/5; UBCH4; UBCH5B
• ACCN: NM_181838
• Insert Size: 1651 bp
• Sequence Data:
  Insert Sequence

  >SC215794 3'UTR clone of NM_181838
  The sequence shown below is from the reference sequence of NM_181838. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGACTCAGAAGTATGCGATGTAATTAAAGAAATTATTGGATAACCTCTACAAATAAAGATAGGGGAACTC
  TGAAAGAGAAAGTCCTTTTGATTTCCATTTGACTGCTTTCTATGAGCCCACGCCTCATCTTCCCCTGTGC
  ACATGTTTACCTGATACAGCAGTGCTGCGTGTTGTACATACTTGGAACAACAAACTAGAAATACTGTACT
  TCTGTACCAACATTGCCTCCTAGCAGAGAAGTGTGTGTGTGACAAGCCAGTTCTACAGGCATTACCTAGG
  TGTGAGACTAAAAGCTTTTCTTATTGACTTAAATTTGGATAACAGCAAGGTGTGAGGGGGGTGGTGGGTA
  TGGTGTGTGCTTGGATGGGAAAGAAAAGGCTCCACTCACCTATAGGAGATTATTTTTAAGTGGAATCCAT
  TTAAACTCAAAACAGTTATGAAAAGCAAGGTGAAGAACATGAAGCTGTGTCTGTATTCATTTTATTCCGA
  AGGAGCTACGTCTTAGGTGAAAGTTATGACCAACCAGATTAAACTCTACCCACATCCTGCATTTTAAGGT
  CTAAGTTTAACTGGTCAACATTTAAATGGATTGGAGCTATTAGTACATCAAGTGTGATGGGCTTTGTTCC
  CAACTCTTTTACATCTCCCTACCCCTTCAACCTTTGGCCTTTCAGCCCTTCTTTCTCTCTTCCATATTCT
  TTGGTTTGTATGTGGTTTCTCAGTTAATACATAGCTAATAGCTCTTATTTTTCTTATGTTTTTAACCGCT
  TAGGTCTATTTGGATGTAAGGGTGAAAATTCATTTGATGGAAATACTTGTGTATATTTAAAGACCCAATT
  GCTCCTCTGGAGCTTGTACTTTCAAGAATGATTAATCTGTGTAATAAACTGGTTACTACAGTCATTACAT
  ATAATTTTGTGTGAATAGGCTTTTTCATTTTTAAGAAGTTTGTCTAGCTGAGATTAGTGGTGGATTTTCT
  CCCACTTCTGAAATGTTCATTTATACTGGTTGCATTTTAAGATCATGAAACAATTCCAGTTACATTGTAA
  AAAGGATATCTTACGAGTAATTTTATTGAACAAGTTAGAGGCATAAGCTTAAGAGCATTTCCATGAAACA
  ACACATGCAGCATTCCAGGAACTTGATTGTTAAATTCAATAAGAAATTTGCTTTATTAATGAAACTAAGC
  TGCATTTCATCAAAACCTTGTGACATTCCCTTGGTACATAGGACATAAAACACAGAGGCATTGCTATTTG
  GTAAGTTAAGCTTCTGTGATTGTAATTATAAAAGAGCAACATTGACCAAACCTGGGAAACAAGAGCACAG
  TCTTGTTTGGAGAGTCTACATAATTACTTTGCACTAACATTTGCAGGATGTTCACACAATTTTAAATTGT
  ACTGTATGTGGCTTTTTGAAGTCTTCCCTTGACCCTAGTAAAATATAGCTTGAAACTTGTAAACAACTGT
  GTTTGCCAGAAACATCATTCATGTGAACTAGGCAAGTTACCTTTTTTCCCCCCTTCTTTTCCTAATTGTA
  AACTAGGCCAACCTGAAAGCCATGGCTGATGCTCTAGCCATCAGGTTCTTTCAAATGCATCTTTACACTC
  TTGCACAAAAGTTAAGGAATAAATGTCCACTGCTTTTGGTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_181838.1
• Summary: 'Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]'
• Locus ID: 7322