TPM4 (NM_001145160) Human 3' UTR Clone

CAT#: SC215852

3`UTR clone of tropomyosin 4 (TPM4) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TPM4
• Synonyms: HEL-S-108
• ACCN: NM_001145160
• Insert Size: 1672 bp
• Sequence Data:
  Insert Sequence

  >SC215852 3'UTR clone of NM_001145160
  The sequence shown below is from the reference sequence of NM_001145160. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACTGGATCAGACACTAAACGAACTTAACTGTATATAAGCAAAACAGAAGAGTCTTGTTCCAACAGAAACT
  CTGGAGCTCCGTGGGTCTTTCTCTTCTCTTGTAAGAAGTTCCTTTTGTTATTGCCATCTTCGCTTTGCTG
  GAAATGTCAAGCAAATTATGAATACATGACCAAATATTTTGTATCGGAGAAGCTTTGAGCACCAGTTAAA
  TCTCATTCCTTCCCTTTTTTTTTCAAATGGCACCAGCTTTTTCAGCTCTCTTATTTTTTCCTTAAGTAGC
  ATTTATTCCTAAGGTAGGCAGGGTATTTCCTAGTAAGCATACTTTCTTAAGACGGAGGCCATTTGGTTCC
  TGGGAGAATAGGCAGCCCCACACTTTGAAGAATACAGACCCCAGTATCTAGTCGTGGATATAATTAAAAC
  GCTGAAGACCATAACCTTTTGGGTCAACTGTTGGTCAAACTATAGGAGAGACCAGGGACCATCACATGGG
  TAGGGATTTTCCATCCAGAGCCAATAAAAGGACTGGTGGGGGCCGGGGGTGGCTATTGTGGGAAGTCATA
  ACCCACAGATAGATCAACCTAAGAATCCTGGCCCTTCTCCACTCTCCACCATGCAGGACAAACATCTTCT
  CAAGCAGTCAACGTAGAATGCTTGGGAAATAGTCATAATTACCCACATATAGTAATTAATAGATGGTAAT
  TAATTGATCCTTGATGTGATGTTCTTTTGCATATTTCCTTCATTCTAAAGTTGTTCCCTGGCCGGGAGCG
  TTGGCTTTCGCCTGTAATCCCAACACTTTGGGAGGCCAGGACAGATCACTTGAGGTCAGGAGTTCGAGAC
  CAGCCCAGCCAACATGGCGAAACCATGTCTCTACTAAAAATACAAAAATTATGGTGACGCCTGCCTGTAG
  TCCCAGCTACTCGGGAGGCTGAGGCAGGAGGATCGCTTGAACCCAGGAAGTGGAGACTGCAGTGAGCCGA
  TATCGCACCACAGCGCTCCAGCCTGGTCGACAGAGTGAGACTCCATCTCAAGAAAAAATAAAAATAAAGT
  TGTTCTCTGAAGAGCAAATGTCTCATTCCAGTAATGACCCACTCAGCAGGAATATGGTGGAGTTCAGTCC
  AATTCAGGTCAGCCATATCCAAAAGACCACAAGTCATTACTAAGTTGAGCAAAAGAGTTTTTATCTATTA
  GCAGAAAGGGCCTCTCTGGCAGCAGAGATTAAAAACTGGCCCAACTTCATTTCCATACTTCAGGGAACAG
  CAAATTGAGGATTTACTTATCTAGGACTTGAATTCCTTCTTTGGGACCAAGTTAATAAAAGACCAAGAAA
  CTCCTGATTAAACTGGATAATGAAGGATTCTGTAGACAGGGCTGCACGTATCGGCTTTGTTTGACTTCTC
  TTTTCTCAGTTAACATCTCAGAGCTAGAACATTCCACATTCCCCAGCAGCGTGTGGGGGCTGACTAAAGT
  TTACAATTCCAACTAAAAATCACCCTGCTTCTGGCTTATCTGAATCCCTTACCCACCCCACCCCACCACC
  CTACTCCTATTTATTCAGCACCACACTACCCAGGAAATACACTAGCAAATTGTGCAATGGAATAAAATCC
  ACACTTTAGATTCTTGCAACTGTATCATATGTAATAGTATCACTTTTTCTACATTTTGGTCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001145160.1
• Summary: 'This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]'
• Locus ID: 7171