FADS2 (NM_004265) Human 3' UTR Clone

CAT#: SC215878

3`UTR clone of fatty acid desaturase 2 (FADS2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FADS2
• Synonyms: D6D; DES6; FADSD6; LLCDL2; SLL0262; TU13
• ACCN: NM_004265
• Insert Size: 1657
• Sequence Data:
  Insert Sequence

  >SC215878 3'UTR clone of NM_004265
  The sequence shown below is from the reference sequence of NM_004265. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGACGCCTACCTTCACAAATGAAGCCACAGCCCCCGGGACACCGTGGGGAAGGGGTGCAGGTGGGGTGA
  TGGCCAGAGGAATGATGGGCTTTTGTTCTGAGGGGTGTCCGAGAGGCTGGTGTATGCACTGCTCACGGAC
  CCCATGTTGGATCTTTCTCCCTTTCTCCTCTCCTTTTTCTCTTCACATCTCCCCCATAGCACCCTGCCCT
  CATGGGACCTGCCCTCCCTCAGCCGTCAGCCATCAGCCATGGCCCTCCCAGTGCCTCCTAGCCCCTTCTT
  CCAAGGAGCAGAGAGGTGGCCACCGGGGGTGGCTCTGTCCTACCTCCACTCTCTGCCCCTAAAGATGGGA
  GGAGACCAGCGGTCCATGGGTCTGGCCTGTGAGTCTCCCCTTGCAGCCTGGTCACTAGGCATCACCCCCG
  CTTTGGTTCTTCAGATGCTCTTGGGGTTCATAGGGGCAGGTCCTAGTCGGGCAGGGCCCCTGACCCTCCC
  GGCCTGGCTTCACTCTCCCTGACGGCTGCCATTGGTCCACCCTTTCATAGAGAGGCCTGCTTTGTTACAA
  AGCTCGGGTCTCCCTCCTGCAGCTCGGTTAAGTACCCGAGGCCTCTCTTAAGATGTCCAGGGCCCCAGGC
  CCGCGGGCACAGCCAGCCCAAACCTTGGGCCCTGGAAGAGTCCTCCACCCCATCACTAGAGTGCTCTGAC
  CCTGGGCTTTCACGGGCCCCATTCCACCGCCTCCCCAACTTGAGCCTGTGACCTTGGGACCAAAGGGGGA
  GTCCCTCGTCTCTTGTGACTCAGCAGAGGCAGTGGCCACGTTCAGGGAGGGGCCGGCTGGCCTGGAGGCT
  CAGCCCACCCTCCAGCTTTTCCTCAGGGTGTCCTGAGGTCCAAGATTCTGGAGCAATCTGACCCTTCTCC
  AAAGGCTCTGTTATCAGCTGGGCAGTGCCAGCCAATCCCTGGCCATTTGGCCCCAGGGGACGTGGGCCCT
  GCAGGCTGCAGGAGGGCACTGGAGCTGGGAGGTCTCGTCCCAGCCCTCCCCATCTCGGGGCTGCTGTGTG
  GACGGCGCTGCCTCAGGCACTCTCCTGTCTGAACCTGCCCTTACTGTGTTTAACCTGTTGCTCCAGGATG
  CATTCTGATAGGAGGGGGCGGCAGGGCTGGGCCTTGTGACAATCTGCCTTTCACCACATGGCCTTGCCTC
  GGTGGCCCTGACTGTCAGGGAGGGCCAGGGAGGCAGAGCGGGAGGGAGTCTCAGGAGGAGGCTGCCCTGA
  GGGGCTGGGGAGGGGGTACCTCATGAGGACCAGGGTGGAGCTGAGAAGAGGAGGAGGTGGGGGCTGGAGG
  TGCTGGTAGCTGAGGGGACGGGCAAGTGAGAGGGGAGGGAGGGAAGTCCTGGGAGGATCCTGAGCTGCTG
  TTGCAGTCTAACCCACTAATCAGTTCTTAGATTCAGGGGAAGGGCAGGCACCAACAACTCAGAATGGGGG
  CTTTCGGGGAGGGCGCCTAGTCCCCCCAGCTCTAAGCAGCCAGGAGGGACCTGCATCTAAGCATCTGGGT
  TGCCATGGCAATGGCATGCCCCCCAGCTACTGTATGCCCCCGACCCCCGCAGAGGCAGAATGAACCCATA
  GGGAGCTGATCGTAATGTTTATCATGTTACTTCCCCACCCCTACATT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_004265.2
• Summary: The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
• Locus ID: 9415