LAR (PTPRF) (NM_130440) Human 3' UTR Clone

CAT#: SC215905

3`UTR clone of protein tyrosine phosphatase receptor type F (PTPRF) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PTPRF
• Synonyms: BNAH2; LAR
• ACCN: NM_130440
• Insert Size: 1694 bp
• Sequence Data:
  Insert Sequence

  >SC215905 3'UTR clone of NM_130440
  The sequence shown below is from the reference sequence of NM_130440. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCAGCTTTGACCACTATGCAACGTAACTACCGCTCCCCTCTCCTCCGCCACCCCCGCCGTGGGGCTCCGG
  AGGGGACCCAGCTCCTCTGAGCCATACCGACCATCGTCCAGCCCTCCTACGCAGATGCTGTCACTGGCAG
  AGCACAGCCCACGGGGATCACAGCGTTTCAGGAACGTTGCCACACCAATCAGAGAGCCTAGAACATCCCT
  GGGCAAGTGGATGGCCCAGCAGGCAGGCACTGTGGCCCTTCTGTCCACCAGACCCACCTGGAGCCCGCTT
  CAAGCTCTCTGTTGCGCTCCCGCATTTCTCATGCTTCTTCTCATGGGGTGGGGTTGGGGCAAAGCCTCCT
  TTTTAATACATTAAGTGGGGTAGACTGAGGGATTTTAGCCTCTTCCCTCTGATTTTTCCTTTCGCGAATC
  CGTATCTGCAGAATGGGCCACTGTAGGGGTTGGGGTTTATTTTGTTTTGTTTTTTTTTTTCTTGAGTTCA
  CTTTGGATCCTTATTTTGTATGACTTCTGCTGAAGGACAGAACATTGCCTTCCTCGTGCAGAGCTGGGGC
  TGCCAGCCTGAGCGGAGGCTCGGCCGTGGGCCGGGAGGCAGTGCTGATCCGGCTGCTCCTCCAGCCCTTC
  AGACGAGATCCTGTTTCAGCTAAATGCAGGGAAACTCAATGTTTTTTTAAGTTTTGTTTTCCCTTTAAAG
  CCTTTTTTTAGGCCACATTGACAGTGGTGGGCGGGGAGAAGATAGGGAACACTCATCCCTGGTCGTCTAT
  CCCAGTGTGTGTTTAACATTCACAGCCCAGAACCACAGATGTGTCTGGGAGAGCCTGGCAAGGCATTCCT
  CATCACCATCGTGTTTGCAAAGGTTAAAACAAAAACAAAAAACCACAAAAATAAAAAACAAAAAAAACAA
  AAAACCCAAGAAAAAAAAAAAGAGTCAGCCCTTGGCTTCTGCTTCAAACCCTCAAGAGGGGAAGCAACTC
  CGTGTGCCTGGGGTTCCCGAGGGAGCTGCTGGCTGACCTGGGCCCACAGAGCCTGGCTTTGGTCCCCAGC
  ATTGCAGTATGGTGTGGTGTTTGTAGGCTGTGGGGTCTGGCTGTGTGGCCAAGGTGAATAGCACAGGTTA
  GGGTGTGTGCCACACCCCATGCACCTCAGGGCCAAGCGGGGGCGTGGCTGGCCTTTCAGGTCCAGGCCAG
  TGGGCCTGGTAGCACATGTCTGTCCTCAGAGCAGGGGCCAGATGATTTTCCTCCCTGGTTTGCAGCTGTT
  TTCAAAGCCCCCGATAATCGCTCTTTTCCACTCCAAGATGCCCTCATAAACCAATGTGGCAAGACTACTG
  GACTTCTATCAATGGTACTCTAATCAGTCCTTATTATCCCAGCTTGCTGAGGGGCAGGGAGAGCGCCTCT
  TCCTCTGGGCAGCGCTATCTAGATAGGTAAGTGGGGGCGGGGAAGGGTGCATAGCTGTTTTAGCTGAGGG
  ACGTGGTGCCGACGTCCCCAAACCTAGCTAGGCTAAGTCAAGATCAACATTCCAGGGTTGGTAATGTTGG
  ATGATGAAACATTCATTTTTACCTTGTGGATGCTAGTGCTGTAGAGTTCACTGTTGTACACAGTCTGTTT
  TCTATTTGTTAAGAAAAACTACAGCATCATTGCATAATTCTTGATGGTAATAAATTTGAATAATCAGATT
  TCTTACAAACCAGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_130440.2
• Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]'
• Locus ID: 5792