RFX2 (NM_134433) Human 3' UTR Clone

CAT#: SC215919

3`UTR clone of regulatory factor X 2 (influences HLA class II expression) (RFX2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RFX2
• Synonyms: FLJ14226
• ACCN: NM_134433
• Insert Size: 1696 bp
• Sequence Data:
  Insert Sequence

  >SC215919 3'UTR clone of NM_134433
  The sequence shown below is from the reference sequence of NM_134433. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TAAAGCGGGAGCGCAGTGACCCCAACCACTCCCTGCAGGGCATCTAGCAGCCCCGGCCGGCGCCTCCTCG
  AGGTTCCAAAAGATGCCGCCTGGTCACTCTGGGAACCTGGATTTCACCGGCTCCACCAAATTAGTGCCTC
  TTAGATGATGTGATGTTTACTTTGACTCAAGCGGGGGCTCCCGGGGTCAGTGTTCAAGAAGGAAAGCAGT
  TGTTGAAGCTACAGAAGCCCAGGCCAGGGCTCCCACTGGCTGTAGCTGTGCTCTGGGTCCCCAGGTCCCT
  GCGCAGCCACCAGGGCCCCACCAGACCATGGGTTCCTTATTCTGGACAAACCCTCCTCTGCTGGAACCCT
  GGACTTTGGCCCCAGTCCCTTTAAGAGCTGAAAGGGCTGTGCTGAGAGCACTGGCCTCTGCACTCAACTG
  GAACCAATTTTCACCATGATTTTAAAAATTAAAAAAACCTGGCAGAGCCCGACTAGAAAGGGACGGCCTC
  TCCCCTCCTACCTGCCACCGCTGGGGAGGCACAGAGCTCGCCAGCGTCCCTGGAGTCCCCTCAGGAATGG
  AAAGTGGAGTGCCCTTGTCTGGTAGCACCATGTGGGAGGGACCCAGCTGGGCGCAGCGCCCTGTGGCCTT
  TTAGATCCAGACCTCCCTGCCGGATGCCCCGAGGCGGGAGGCCGGCTGTGCTGCAGGAACCCATCTCCAG
  ATGCCAAAGGACTTGAGGGGCAGCTGACAATCGCTGTGTCCCGGCAGATCCGCAGCTCGAAAAAGAACAA
  GCCACAGAAACGGGCTCGCTCGTGCCAGGACACAGCAGTGTCTTTCAAAAAATCAAAACCAGAAGTTTTA
  TCAGCAGCAGGAAGGATGTGGGACTCTGTCCAAGTTCACCGTCACCATCAAGCCACTGGCTGTGGAAGGA
  GTTTGTCCAACAGGGTCAGTGTCACAGCCACAACTTCAGAAAGCAGCCATCCCGCGTGTCGTCCAAACAG
  CAGTGCCTGCGTCCCGCTCCACGGAGTCTTCCAGAACACCTCCCTCTGAACTAATCCCGGAGCACCCCAC
  CCCCAGCGAGTCCCACCTGGACCTCTTCAAAGTCAAAACTCTTCTCTCGGGACAAACAAATATTCGGGAC
  TTTCGAAGCCCTGAACTTGAGACACCAAGTTTGTCTCCATGGAAACACGTAGCCGCTGCAGTTGGACAGC
  TGCGTGCTGGAGTTCTGTCCCCAAACCAACTGCAGAGAAGAAAGACGAGCCTCGGCCCAGGACGCGATTT
  CTAACGAAATGCAATAGTTTTCTCTTTTTGGAAAAATGTTTATACCAATAGTTTTGTGCGCTAATTGTTA
  TTAGAACGTTTCTTATGAATGTGTACATTGCAGTATATTCAGTCCCAAATGTTTTGTTCTCATTCACACA
  GAAGGTACTAATGTCAGCTCACGTGGCCTTTAAAAACCTACTTCCCCGCCCGCAGGAGCTCAGAGCATCG
  CTGGGCCTGCGCCAGAGTGAGGGGTGCAGGAAGACAAAGACGCGTGCTCACTTTAGAAACGAAAAGCTGG
  GAATAATCACACTGTTTGTCTGGTCGTATAGCCCAAACCTTAGTTGAAATCAGACAGAAAGAATGTCTTT
  AAAATATACATCTCCTCTCCTTGGAGACACTGAAGCTCACAGTTAACGGATTGTAATGCCCGGCTTTTCC
  TCACTCCTGGTAACCA
  
  CGGACCGTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_134433.2
• Summary: 'This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene, and both variants utilize alternative polyadenylation sites. [provided by RefSeq, Jul 2008]'
• Locus ID: 5990