PDGF beta (PDGFB) (NM_033016) Human 3' UTR Clone

CAT#: SC215944

3`UTR clone of platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) (PDGFB) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PDGFB
• Synonyms: c-sis; IBGC5; PDGF-2; PDGF2; SIS; SSV
• ACCN: NM_033016
• Insert Size: 1694 bp
• Sequence Data:
  Insert Sequence

  >SC215944 3'UTR clone of NM_033016
  The sequence shown below is from the reference sequence of NM_033016. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGACAAGACGGCACTGAAGGAGACCCTTGGAGCCTAGGGGCATCGGCAGGAGAGTGTGTGGGCAGGGTTA
  TTTAATATGGTATTTGCTGTATTGCCCCCATGGGGTCCTTGGAGTGATAATATTGTTTCCCTCGTCCGTC
  TGTCTCGATGCCTGATTCGGACGGCCAATGGTGCTTCCCCCACCCCTCCACGTGTCCGTCCACCCTTCCA
  TCAGCGGGTCTCCTCCCAGCGGCCTCCGGCGTCTTGCCCAGCAGCTCAAGAAGAAAAAGAAGGACTGAAC
  TCCATCGCCATCTTCTTCCCTTAACTCCAAGAACTTGGGATAAGAGTGTGAGAGAGACTGATGGGGTCGC
  TCTTTGGGGGAAACGGGCTCCTTCCCCTGCACCTGGCCTGGGCCACACCTGAGCGCTGTGGACTGTCCTG
  AGGAGCCCTGAGGACCTCTCAGCATAGCCTGCCTGATCCCTGAACCCCTGGCCAGCTCTGAGGGGAGGCA
  CCTCCAGGCAGGCCAGGCTGCCTCGGACTCCATGGCTAAGACCACAGACGGGCACACAGACTGGAGAAAA
  CCCCTCCCACGGTGCCCAAACACCAGTCACCTCGTCTCCCTGGTGCCTCTGTGCACAGTGGCTTCTTTTC
  GTTTTCGTTTTGAAGACGTGGACTCCTCTTGGTGGGTGTGGCCAGCACACCAAGTGGCTGGGTGCCCTCT
  CAGGTGGGTTAGAGATGGAGTTTGCTGTTGAGGTGGCTGTAGATGGTGACCTGGGTATCCCCTGCCTCCT
  GCCACCCCTTCCTCCCCACACTCCACTCTGATTCACCTCTTCCTCTGGTTCCTTTCATCTCTCTACCTCC
  ACCCTGCATTTTCCTCTTGTCCTGGCCCTTCAGTCTGCTCCACCAAGGGGCTCTTGAACCCCTTATTAAG
  GCCCCAGATGATCCCAGTCACTCCTCTCTAGGGCAGAAGACTAGAGGCCAGGGCAGCAAGGGACCTGCTC
  ATCATATTCCAACCCAGCCACGACTGCCATGTAAGGTTGTGCAGGGTGTGTACTGCACAAGGACATTGTA
  TGCAGGGAGCACTGTTCACATCATAGATAAAGCTGATTTGTATATTTATTATGACAATTTCTGGCAGATG
  TAGGTAAAGAGGAAAAGGATCCTTTTCCTAATTCACACAAAGACTCCTTGTGGACTGGCTGTGCCCCTGA
  TGCAGCCTGTGGCTTGGAGTGGCCAAATAGGAGGGAGACTGTGGTAGGGGCAGGGAGGCAACACTGCTGT
  CCACATGACCTCCATTTCCCAAAGTCCTCTGCTCCAGCAACTGCCCTTCCAGGTGGGTGTGGGACACCTG
  GGAGAAGGTCTCCAAGGGAGGGTGCAGCCCTCTTGCCCGCACCCCTCCCTGCTTGCACACTTCCCCATCT
  TTGATCCTTCTGAGCTCCACCTCTGGTGGCTCCTCCTAGGAAACCAGCTCGTGGGCTGGGAATGGGGGAG
  AGAAGGGAAAAGATCCCCAAGACCCCCTGGGGTGGGATCTGAGCTCCCACCTCCCTTCCCACCTACTGCA
  CTTTCCCCCTTCCCGCCTTCCAAAACCTGCTTCCTTCAGTTTGTAAAGTCGGTGATTATATTTTTGGGGG
  CTTTCCTTTTATTTTTTAAATGTAAAATTTATTTATATTCCGTATTTAAAGTTGTAAAAAAAAATAACCA
  CAAAACAAAACCAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_033016.2
• Summary: 'This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]'
• Locus ID: 5155