Plakophilin 2 (PKP2) (NM_001005242) Human 3' UTR Clone

CAT#: SC215948

3`UTR clone of plakophilin 2 (PKP2) transcript variant 2a for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PKP2
• Synonyms: ARVD9
• ACCN: NM_001005242
• Insert Size: 1686 bp
• Sequence Data:
  Insert Sequence

  >SC215948 3'UTR clone of NM_001005242
  The sequence shown below is from the reference sequence of NM_001005242. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACTGCCAAAGCCTACCACTCCCTTAAAGACTGAGGAAAATGACAAAGTATTCTCGGCTGCAAAAATCCCC
  AAAGGAAAACACCTATTTTTCTACTACCCAGCCCAAGAAACCTCAAAAGCATGCCTTGTTTCTATCCTTC
  TCTATTTCCGTGGTCCCCTGAATCCAGAAAACAAATAGAACATAATTTTATGAGTCTTCCAGAAGACCTT
  TGCAAGTTTGCCACCAGTAGATACCGGCCACAGGCTCGACAAATAGTGGTCTTTGTTATTAGGGCTTATG
  GTACATGGCTTCCTGGAATCAAAATGTGAATTCATGTGGAAGGGACATTAATCCAATAAATAAGGAAAGA
  AGCTGTTGCATTACTGGGATTTTAAAAGTTTGATTTACATTTATATTCCTTTTCTGGTTCCCATGTTTTG
  TCACTCATGTGCACATTGCTTCGCCATTGGGCCTCCAGTGTATTGTTCTGCAGTGTTGAAACAGAATGGA
  AATGACAAGAAATATCTGCAGTTATCCAGGAGAAAGTATAATGGCAAAATTATTGGTTTCTTTCTTTACT
  TTGTGCTTGTTTTTATCCCCTTGGGTTGTTTTTCTCTGATTTTTAAATAAACTTAAGAAATTTAGATTAC
  AGAGTATGCATGACTGTAAGAAAAAGAAATTGAGAGGAAGTGATCATAGCAAATTAAAGAAGTCTTTTCC
  TCCCAGAACTTAAAGTAAAATAAAAAATAAATAAATAAATAAAATCTTTTCCACAGAGAAAGGCAACTGT
  GATGATAAAATTTAACGTTCCCCCAAACACTGAGTCAATGAGATTTTTCTCAGGAGATACTTTACCTATA
  ACAACGCCGTTAAATCCAAATCTCTTCTAAACGATGGCATTCTATGTAATGCCTTTCCTGGACTTTTTTG
  GCCACTGCCCTGGACTAGTGAAAGAATGGACTCTATCTTTATCTGCAAGAGGAACTAAGGCCTTCTCTCA
  GACTGCCTGGCCAGCCTGGGGCACTGAAAATACGGCTCATGTTAATGAGTTACATTATCAGCCAGCCCAG
  CCTTGCCCACCATTTAAGAAATATCACAGAGCCACTAGATCTCATATGATCTTCTTCAAGCCATTATTTT
  AACTCAAGAAAACTCTAGAGAAGAAAAGTGAAGAAGTCATGTTGAAGAAGATGTAAGAATGTGTCAAGAC
  CATCCAGAAATGATATGAGAAATACTGATATTTTAAATGGTTGACATCATCCAGCGAAATGAATCTACAT
  TAAATGTTGTTTTAACTGCGCTATGATTAAAACCATTCATATAGAGTTAGTCTTTACAACTACTATTCTG
  TTATTTTTTTTTTTAATCTGACAACATTTGTCCTAAGTAAGATAAGCAAAAAAATTCTTCAACTCCTTTT
  GGCAAGAAAACTGTAACAGAAAATAAATTTTGAATGTGTACTTAAGTCTTTATTATATTTGAAGCAATTT
  TTTTTCAATTTTAAAAGCTGAATGAAGACAACTTAGGTTGCTAACCTAGTTCAAAATGAAATTATTTAGA
  TACCAATTTTTAAAATACTGGAGAGAATTTATATGTCTTTTTCCAGAGTTCTGATGATAAGCATTTGGAG
  TGCATTTATTCCTCCAGATAATAAATGTGTGTTCAGAACTTTTTGTGTTTTTTAAGGCATTAATAAAGCC
  TTCGAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001005242.2
• Summary: 'This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13. [provided by RefSeq, Jul 2008]'
• Locus ID: 5318