STAT1 (NM_007315) Human 3' UTR Clone

CAT#: SC215993

3`UTR clone of signal transducer and activator of transcription 1 91kDa (STAT1) transcript variant alpha for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: STAT1
• Synonyms: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• ACCN: NM_007315
• Insert Size: 1685 bp
• Sequence Data:
  Insert Sequence

  >SC215993 3'UTR clone of NM_007315
  The sequence shown below is from the reference sequence of NM_007315. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGATAGTGGGCTCTGTAGAATTCGACAGTATGATGAACACAGTATAGAGCATGAATTTTTTTCATCTTCT
  CTGGCGACAGTTTTCCTTCTCATCTGTGATTCCCTCCTGCTACTCTGTTCCTTCACATCCTGTGTTTCTA
  GGGAAATGAAAGAAAGGCCAGCAAATTCGCTGCAACCTGTTGATAGCAAGTGAATTTTTCTCTAACTCAG
  AAACATCAGTTACTCTGAAGGGCATCATGCATCTTACTGAAGGTAAAATTGAAAGGCATTCTCTGAAGAG
  TGGGTTTCACAAGTGAAAAACATCCAGATACACCCAAAGTATCAGGACGAGAATGAGGGTCCTTTGGGAA
  AGGAGAAGTTAAGCAACATCTAGCAAATGTTATGCATAAAGTCAGTGCCCAACTGTTATAGGTTGTTGGA
  TAAATCAGTGGTTATTTAGGGAACTGCTTGACGTAGGAACGGTAAATTTCTGTGGGAGAATTCTTACATG
  TTTTCTTTGCTTTAAGTGTAACTGGCAGTTTTCCATTGGTTTACCTGTGAAATAGTTCAAAGCCAAGTTT
  ATATACAATTATATCAGTCCTCTTTCAAAGGTAGCCATCATGGATCTGGTAGGGGGAAAATGTGTATTTT
  ATTACATCTTTCACATTGGCTATTTAAAGACAAAGACAAATTCTGTTTCTTGAGAAGAGAATATTAGCTT
  TACTGTTTGTTATGGCTTAATGACACTAGCTAATATCAATAGAAGGATGTACATTTCCAAATTCACAAGT
  TGTGTTTGATATCCAAAGCTGAATACATTCTGCTTTCATCTTGGTCACATACAATTATTTTTACAGTTCT
  CCCAAGGGAGTTAGGCTATTCACAACCACTCATTCAAAAGTTGAAATTAACCATAGATGTAGATAAACTC
  AGAAATTTAATTCATGTTTCTTAAATGGGCTACTTTGTCCTTTTTGTTATTAGGGTGGTATTTAGTCTAT
  TAGCCACAAAATTGGGAAAGGAGTAGAAAAAGCAGTAACTGACAACTTGAATAATACACCAGAGATAATA
  TGAGAATCAGATCATTTCAAAACTCATTTCCTATGTAACTGCATTGAGAACTGCATATGTTTCGCTGATA
  TATGTGTTTTTCACATTTGCGAATGGTTCCATTCTCTCTCCTGTACTTTTTCCAGACACTTTTTTGAGTG
  GATGATGTTTCGTGAAGTATACTGTATTTTTACCTTTTTCCTTCCTTATCACTGACACAAAAAGTAGATT
  AAGAGATGGGTTTGACAAGGTTCTTCCCTTTTACATACTGCTGTCTATGTGGCTGTATCTTGTTTTTCCA
  CTACTGCTACCACAACTATATTATCATGCAAATGCTGTATTCTTCTTTGGTGGAGATAAAGATTTCTTGA
  GTTTTGTTTTAAAATTAAAGCTAAAGTATCTGTATTGCATTAAATATAATATGCACACAGTGCTTTCCGT
  GGCACTGCATACAATCTGAGGCCTCCTCTCTCAGTTTTTATATAGATGGCGAGAACCTAAGTTTCAGTTG
  ATTTTACAATTGAAATGACTAAAAAACAAAGAAGACAACATTAAAACAATATTGTTTCTAATTGCTGAGG
  TTTAGCTGTCAGTTCTTTTTGCCCTTTGGGAATTCGGCATGGTTTCATTTTACTGCACTAGCCAAGAGAC
  TTTAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_007315.3
• Summary: 'The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C. [provided by RefSeq, Jun 2020]'
• Locus ID: 6772