WARS2 (NM_015836) Human 3' UTR Clone

CAT#: SC216056

3`UTR clone of tryptophanyl tRNA synthetase 2 mitochondrial (WARS2) nuclear gene encoding mitochondrial protein transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: WARS2
• Synonyms: mtTrpRS; NEMMLAS; TrpRS
• ACCN: NM_015836
• Insert Size: 1709
• Sequence Data:
  Insert Sequence

  >SC216056 3'UTR clone of NM_015836
  The sequence shown below is from the reference sequence of NM_015836. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGTGTGCCAGGAGGTGAAGAAATTGGTGGGTTTTCTATAGGAAGTTTCAACGAATCACAGCAAGGCTTTT
  GTGCCTTGCACTCCATGCATTCTGATAACGGCAGCTTTCCTAAAAAGAAAAAGTTATAGTTTTGGGACAT
  TTAATTTGGTATAGCTGATTATTGGCTTTATTTGATGAATATTGCTTTGTAGCTTTGAAATACGACAGTG
  TTCCAAATCCCATCAACAAAATGCTGTGAACAACAACAACAAAAAATAAATCAAGAAGGCATAGCTGTCT
  GAATCCCCAATTATTGAGGCACACTCCTTGGCCTGCAGAAATGTGATTGAAGTAAGGAGTAATTTGGGAA
  ATGGAGTATCATTTGTGCTTCTCTCTGGAGTACTTACTGTAACAGATGCACACGTTATCTACTTCAACCC
  TTTTATAAAGCAATATATAGAGGTCTTAGATGTATAATCCTTGAGATGCCATTTGATCAGATGCCAATTT
  GTATTCAGAGCTCATAAAAACAAGTCCATTCTTATAGTTTGTTTGTCACTGAGACGGTGCCTGATTTAAT
  ATATTTAAGCTGCTTAACTTGTTTTCCAAGAGATAACACCAACTGTTCCAGCAATAATTTGTTTTTTAAT
  TCAGAGATATGGGATATGATCTTCTAAGGAGGTTGCATTATTTTGACCTGATTTATTTATTGTACAAGAG
  CCCAAGTCCCTGAATTATATTCTTAATAAGTGCTTTCTTGCATTCATTTCAGTTTAGTCTCACCAACCCC
  CTATGAATTAGGAGAATTATTGTCATTTTACACATGAGGAAAGTGAGGCTGAGAGAATCCAATTAGCTTG
  TACGATATCACTCCATAAGAGAAGCAAATCTGAGAGTAAAACCCTTACCCCTTATTCTTAAGAATAGTGC
  TTGTTTATTCAGTACAAAAAAAATGCTGAAAGGCAGCGTTTTTTAGACTCTAAACATTCTGATTTTGAAG
  GTGGGTGTTGGAACGACTGATGATGTTTTTTATATCATCAATTCAGCCAATGGATGCGAACTTAAAATCT
  GGATTCACAGAACCGTGGTCACATTTCCCCATCAAAATCCCCAAGGCGGGTCCCAAGTTCTCTCCTCTAC
  GCTCATTGCAGAAATACTTTGAAATAGGTTCTGCCAGTCTACAATAGCAGTGAAGACTGACAAATCAGAT
  ATTCCCAATGACTGACAGCTTCTCATCTTGCCTCTACCTCAGTTAAGTAATGTCACCCTGAAACCTAGCT
  TTTCTGCACTTCCATTTCTGACACCTGAAAATGAAAATGATAATCCTAACTTATACACCAAGACCTGTTA
  GCAAGATCGATTTTGTAACAGAAATTGCTTATAAGTTTTTTATCAGTAAGGACCATGATCACCATTTGTA
  CATACATAACTAGGTTGTTGAGCAAAGCATTTTTGCTCATGTTTCTTAAGGAAGTAGAGTAGTCCTTGTG
  TCAGAACACATGTTAATCCACAAAACGCCCCATCCCAGATTCCAAATTAAAACATCGTTTTTAGGATTCT
  TGGAGCTAGCCTGGATCATGGGTAAAGGAAACATTTTGCCTTTTGTATCTTATACTGGAATGGGATGTCT
  GTAAGTCCTAGAACCTCTTTGAGCATATTTCTTACAAGCCTCTGTTTTTTCCCCTTTAAAGGAAACTTTG
  AAAGGATTGTGAATACTGGAAAATGACAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_015836.3
• Summary: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
• Locus ID: 10352