TAF4B (NM_005640) Human 3' UTR Clone

CAT#: SC216076

3`UTR clone of TAF4b RNA polymerase II TATA box binding protein (TBP)-associated factor 105kDa (TAF4B) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TAF4B
• Synonyms: SPGF13; TAF2C2; TAFII105
• ACCN: NM_005640
• Insert Size: 1687 bp
• Sequence Data:
  Insert Sequence

  >SC216076 3'UTR clone of NM_005640
  The sequence shown below is from the reference sequence of NM_005640. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TTCTCGAGCTCTATACCTGGCCCTTCTGAAGTGACCACTCCACTCTTCCATCCAGATCCTTGCTATTTAC
  TGCCAAAGAAGACACAAAGCATTGTTGCACTGTCCTGAAATTTCAATTTCTGGAAAATAATCACCAACAT
  GAAAGAGCATTGTTTACAGTTAGAAACTTTATTAACTCTTACCTATCCATCTCATGGGACTCTTACAGAC
  TCAGATTCATCTTTGTCTTCTGAAAATCAGTTATGAAATACACTTTGCACAGAATTAGGCATCTGCCTAT
  CTGTGCATTAAATTAAAGCAAGTTAAGGCCCTATTTGTTACTACCTGCCTCATTTGCCAAATTGTAGCAG
  GTGAGGTGTCCTTCCCTAATACAGCATGCATTGAGATGCAGGAGAAAGGAAGAGGCATAAGAAAATACAA
  GAATGACTATTTCATTCTATATTGAACCTGTCCCAAAGTGGTAGGTTTTCTGGGCTGGAATCAGAAGTTG
  CTTAGCTAACTATGACAGTCACCCTCTGTACGTGACAGGCCACTGTGGTGACTTTGGTCGGCTGAGTTCC
  AGTGTTGTCAAAACAAGACAAATGGGGATAGATTGTGCCTGGGGTTGCTAATTCTCTGCTTCATATCACC
  ATATGCCGTTTCTGGTACCAAGTGAGGGGACATGCTTGTCAGGGAAACCTTAAAATTGAATCTACTGATA
  TATCTTGTTGAAAAAATGGAAAAGGTGCAAAGTATTAGAAGGAACGTTAGGGCGTTGCTTTCAAACTGAT
  GAGGGGAATGTGAAAATGGTATCATAAAACAGGAAATGTGCTTCATATGCCCAAGAGGGGAAGAGGTGGA
  ATCGGAACTCCAAAACAACTGGTACATGTTTTGTTGATGGGCAAAAAAAATCACAGCACAACCAGAAAGG
  AGAATGTACCAGATGTTTTCAAATTAGAGTATTTCAAAAGGAAAATGTTATTTTTTGTTTTGTATTGTTT
  TAAATCCCAAGGATAGTTTTTATAGTTCCTTCTGTCATAATATATTTTAGTTATCCTAATTTTTAAGCAT
  GTTGGCACATTTAAAAAATCCTAATTGATGATGAGAAATTTTTAACAATAGGACTTTATGTTGGATTCTG
  TACTTAAAAGGTACTGTCAATATCTCACTCTTGGGGATATGAGCTCTAAATATGAGAAGCAAGTTATATG
  TGTTCACCTGAGGAAGAAGCTGACGGACAAAGCAAACCCTCTTTAAAAAAAAAAAAAAGAAAATTCCACG
  TGTGTGGAATACAGCCGGTGAAAAATAATCACTGTAGTTAGAATCACATACAGAAAAAAAATGATTGAAT
  CCTCCAAGAAGGTATTTCAAAAATGGGAAATGCTATACTTATAAGAATTGAAGGAAAAAAAAAAAAACAC
  CTCACGTATGTTATTCTTCATCCTGTTCTTCCCATTGGGACTGTTATGGCCATATTCTACAGTATAGTAA
  ATTTTAAATTGGCCACATTTTTTTTTGTTTCAGCAAGTTATGTAAAATGCTATAAATTATGTGTATGTTA
  AAGGAGTACTTTAAGAAGAGATCTTTATCCAAAGTTGTTTTTGTATATTTAAATGCTTACCTTTATTTTT
  TGTAAAGTGCAGCATACTCATTTGTGTATAATCTTACTGATCAGATGTTTAAAATTATTCCAAATACTTC
  ATTAAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005640.1
• Summary: 'TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]'
• Locus ID: 6875