EHD2 (NM_014601) Human 3' UTR Clone

CAT#: SC216162

3`UTR clone of EH-domain containing 2 (EHD2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: EHD2
• Synonyms: PAST2
• ACCN: NM_014601
• Insert Size: 1726
• Sequence Data:
  Insert Sequence

  >SC216162 3'UTR clone of NM_014601
  The sequence shown below is from the reference sequence of NM_014601. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTCCAAGCGACGCCACAAGGGCTCCGCCGAGTGAGCCGGCCCCCCTCCCATGGCCCTGCTGTGGCTCCCC
  AGCTCCAGTCGGCTGCACGCACACCCCTGCTCCGGCTCACACACGCCCTGCCTGCCCTCCCTGCCCAGCT
  GTAAGGACCGGGGGTCTCCCTCCTCACTACCGCCAGACACCCCGGTGGAAGCATTTAGAGGGGACCACGG
  GAGGGACAAGGCTTCTCTGTCCGCCCTTCACACCTCCAGCCTCACGTTCACTTAGGCACATCACACACAC
  ACTGGCACACGCAGGCATCCATCCATCCGTCATTCATTCAAATATTTATTGAGCACCTACTATGTGCCCA
  GCCCTGTTCTAGGCACTGGGCATTACCATAGAGAACAAAATAGACAAATACATCTGCCCTCATGGAAGGT
  GACGTTCCCAGGAGAGGGCACCTACACAGTCACGCAAACACACACTAATTCCTGGCAGGGCCCCCAGCCC
  CTCCCCTGGCTGAGCAGCCCTGTGGCTGAAATGACTAGCAGATAAACAGACCCCCTTCTGCTCCGCTTCC
  TCCTGCCCAGCCAGGCAACACCCTCAACCGGCTCCATCACATCCTCAGGTCTCGGGACCATGGGGGGCTC
  AGAGGGGAGACACACCTACTGCTTCCTCAGATGGGCCCCTCCGCAGCCCCTTCCCTTGCTCGGGGAAAGC
  CCCCAATTCTGCCCACACCCATTTATTTCCTTCCTTCCTTCCTTCTTTTCTTTCCTTCCTTCCTTCTTTT
  TTGTTTTTGCCCCCAATTCTGCCCATACCCATTTCTTTCTTTCCTTCCTTCCTTCTTTTTTGTTTTTGCC
  CCCAGTTCTGTCCACACCCCTTCCCTTTCCTGTCCTGTCCTTTCTTTCTTTTTTGATAGAATCTTGCTCT
  GTCGCCCAGGCTGGAGTGCAGTGGTGAGATCTCAGCTCACTGCAACCTCCACCTCCTGGGTTGAAGTGAT
  TCTCGTGCCTCAGCCTCCTGAGTAGCTGGGACTGCAGGCACGCGCCACCACGCCCAGCTAATTTTTGTAT
  TTGAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCGCATCTCAGGTGATCTGCTC
  GCCTTGGCCTCCCAAAGTGATGGGATTACAGGCATGAGCCACCGTGCCCGGCTTCACACCCATTTCTTTA
  AAAAGGATCCCGTAGCAGGCAGAAAAGCCCCTTCCATCCTGCTCCTCTGATACTGTGCCCCCTTGGAGAT
  ATTTCCGTCCTCCACCCACGTGTCTGTGGCTGGAACTGCCCAGCCTGCTCCTGGCCCCCTGGAAGCCTCC
  CCACAGCTGGTAATCTGGACTTAAGGATTGCTGGGCCACCGCCTCTCTGCCTACCACCATTCCATATTTA
  AGTGGAGCCCCTACGTAGAAAGGCCCCGGGGCTTTATTTTAGTCTCCTTTTCAGGGATGTCGTGGGCGGG
  GGAGGGGGTTCTTGGTGCTACAGCCCTCTCCCCACCCCTAAAGGGACGCCGACGCTGTTTGCTGCCTTCA
  CCACATATTAGTGCTTGACCCTGGCAGGGGACCCCATGGAAAAGATGGGGAAGAGCAAAATACATGGAGA
  CGACGCACCCTCCAGGATGCTCGCTGGGATTCCCACGCCCACCACTGTCCCCCACCCCATGGCTGGGAGG
  GGCCTCTGAACGGAACAGTGTCCCCACAGAGCGAATAAAGCCAAGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_014601.3
• Summary: This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]
• Locus ID: 30846