VAMP2 (NM_014232) Human 3' UTR Clone

CAT#: SC216188

3`UTR clone of vesicle-associated membrane protein 2 (synaptobrevin 2) (VAMP2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: VAMP2
• Synonyms: SYB2; VAMP-2
• ACCN: NM_014232
• Insert Size: 1727 bp
• Sequence Data:
  Insert Sequence

  >SC216188 3'UTR clone of NM_014232
  The sequence shown below is from the reference sequence of NM_014232. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TTTGCGCCATCATCCTCATCATCATCATAGTTTACTTCAGCACTTAAATCCCCGAGGAGTCTGCCCTGCC
  TAGAGAAGGGCCTCTCCCCCAACCCTCAGCCGTTCCTCCACCTCTCAGCCATATCTTTCAGCCCCCACTC
  CCCTGGATCCGTGTGTGTGTGTGTCCGTGTGTGTGTCCCCCTGTAAATAGCCAGCTGTTATTTATACATA
  TATAATATTATATATATTTGGTCTGTTTGTAGTTTTATTACTAGATGATTTTTCCGGTTGTCCTTAACAC
  CCCTTCCTGAGGTTCCCTTCACCCCTCTCTCTTGCCTTCCTTCCCTTTCCCTTTCTTCCTGACTAGCCCC
  AAGTCCCTTCATTTGCATCTGCTATGCAATAGTCCCTCTCCTTTCCTTCTTCTTCCCTCAGATTTAGCTG
  ATCCTTCCTCCCACCCTGGCCTTCCTTTCCTCTTTCCTCCTCACTCTCCCCGTCATGCTCCCTCTGCCCC
  GCCCTCAAAAAAAAAAAAAAAAAAAAAAAAACAACAGCACCTGTCCAGGCTTCCTTAGGTACATCTTCTT
  TGTATCCATTGGGAGGCTCTGAGACTGGCCCCACTTGGTCCTAAGAATCCCAAGGTCTTTGGGAGCGTCC
  AGCATGTTAATTAGCGTATCATTACATACTGCTATCCCTTTCCATTTCTTTTTGTTCCATCACTCTTCTC
  TCAACCTGTGTTTCTTTTTTTACTGAGGAGTTAGTCCCCATTAGTTCTTGTATCACATTTTCATTTGCAC
  GACATTACTCGCAGGTGGTGGGGAGCCTGGGCTTTTGGGGAACCAGGCTGCTCTGGTCCCCAGCATTGCC
  TCCTCCTAGCCCCTCTAGTCCAGTTTGCCTCCCTTACCCTCATTTTCCAAACCTCTTGTACCCTCCTCTC
  CCTCCCCCAGCTGGTATGTAAGTGTCTTGAAGTTCAGTATGTTATGATGGACCAATAATTCTGCCACTTC
  GGGTTTCTCCCTACATTCCTGCTCCCCAGTTTTCATGTGGGGTACTCAACTGACATTCCCATGGGGTTTC
  CCTCCCATCTGCCTGATCCACCTCCTCCTCCCACCAGGAGAGTTGGGGGTTGGCCACAATTGATCTCTTG
  TGAGAGGGGTGGCTACCAGTGTGTGTGTGGGGGTCATCACTGCCTTGGGGAGGAGTGGGGCAGGGCAGAG
  AATCCCCCCAATTCCTGCCTGAAATCTCTGGCCTCACCCCTGCTGGGGGTTGGACTGAAAACCCTCCTCC
  CCAATTTGGGGGGTGTTGCCCCATCACTGCCCAGCTCCTCTGACTGCCCCCCCTGAATTTAGGGTGGGGG
  TACTAGTCACTGCCAATGTGTGTATGGGACTTGCTGGAAAACGGGGATGCTTGCCCCTCTCCAGGACTAT
  TGAGCCCAGAGAGAGCTGTCCTCTCATTGGGTGAACTGATTGAGGAAGGGTCTATTGTCTTTTTAAATGG
  CACAATTTTAAGGGTTTGAGGGTACAGTCCCTTAACCTGCCACGGGAGGGGGCCCCCAAACTTTCTTCCC
  CCCACACTTCTGGTTTTCTGTGTGGAGGGGGAGCAGGGATATCTAAGCTGTGGTGTGAAAGGGTAGGAGA
  GATGCTGGAGGTGGGGGTGCTGTGTTTTAGACCCCCCATATTATCCCAGTGTCCCCTGCCCCCCTCTTCC
  CCCACCCCATGCCCCCAATTCTGTGGCGCATCCAGATTGTGAAAATG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_014232.2
• Summary: 'The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008]'
• Locus ID: 6844