DAGK (DGKQ) (NM_001347) Human 3' UTR Clone

CAT#: SC216286

3`UTR clone of diacylglycerol kinase theta 110kDa (DGKQ) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DGKQ
• Synonyms: DAGK; DAGK4; DAGK7
• ACCN: NM_001347
• Insert Size: 1748 bp
• Sequence Data:
  Insert Sequence

  >SC216286 3'UTR clone of NM_001347
  The sequence shown below is from the reference sequence of NM_001347. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGAGAGCGATCCTAGGTAGGGGTGGCTGGGGCAGCCCAAGGGCTCGAGCCATCTCTGCTCCCGCCAGCCT
  TGTTTTCAGGTGGTCTGGAGGCAGCTCCACGTCCACACAGTGGCCGGCCCTGTGGTCGGACCTGGCTACA
  GCCCTGACCACAGCATCTTCCCAGTGACGGAGCGGGACCCGCCCTCATCCCATTGGTGACACATGTTGGT
  GTCACAGCCCTCATCCCACTGGAGACTGGTGTGCGGGTGGCAGTGTTACCCCGTGTCCCGGGTGGGCGCA
  GGGCTCTGGATGGTGAGCCCTCTGCCCTGTGGGGGCCTGGTCCTCTCCTCAGGGGTGGGCACCCATCCCT
  CTACCCACTGAGCCCCTTGAGCTGGACAGTGCTGGATGCAGGCCGTCCAGGTGGGTCTGATCTCACTTTG
  TGCCCCTCGGGCCGTCCCCCTGGCCAGTCCTTCAGAGCAGCAGGCTCCCTGGTGTGTCCCTGCCCCAGCA
  GACACAGGCTGCCAGTCCCTCCAGCCTCACCTCCCCGCCCTGGGCAGCAGGTGTGGGCAGCGTGTGAGGC
  TGCCGGGTGGTGGTGACTGGTGACTTCCTTGTGTTCAGGGAAGGGAGAAGGTGCCTTCCCTTGTTTTCTG
  GCCTTGTTATATACAGATGGCAGCTTGGATCTCAGGTACAGCTCCAGGGGCAGGCAGTGCCCAGCTGGAC
  CTGGTGGCCCTTTCCTAGTGCCTCTGCTTGGGGAGGAGAACCTCTGTCCACGTGGAGGCTAGGAGGTCTC
  AGGTGCTGCCCTGGCAGCACCAGAGTGTGGGCCGGGCCCGAGTGTCTGCCCCTCGGCCCTCAGGGTGGGG
  CACTTAGCACCCAGAAGGGACCAAAAGCAGGGCATGGCGGTGCAGAGGAGTTTGGGAGGTGTAAACAGCC
  CATGCACGTGGAGGAGGAGCTGGCTTTCAGCCCCAGACCCCACGCTAGCACTTTCCACGCTGCTTGCCCG
  CTGTTGATGTGCAGTTCCCAGTGCCTGTGTGAGCCGACATCTGCTCAGTCCTATCCCTCGTCAGCGTGTG
  GAGACCCAGCTCCTGCAGCCCTCCTGCTCCCACGCCCCCAGACAGCTTGGTGGAGGGTCCTGCATCTGGG
  CCAGGCTGGGGTGCACCCAGCCAAAGACAAAGCTGCCTCCACGTGCCCAAGGATTCAGATGGTGCACTGG
  CCCCGGGAGGAGTCTGACCAAAAATGGAGCCCGCTCTGTGGGGAAGCCCCGACTCCCCCACGAGAAACGG
  TCCCACGGTGCGGATCTCCCCCTTCCCTTGTGGGGCACAGCTGGCCTGGGCCTCCAATCCTGCGGAGCTT
  TCCTGGGTGTGGCTTTGACCTCAGAAGTGGCTCTGGTTTGGCCTCAGGAGTGTGGCCTGGCCCAGCCTGC
  TGCAGCCTCCTGGGGGGCCCTTGATGCCACTAATCCCCCGACCCCCCGCATCTGCCAAACTGCACAGACA
  CACGCATTGTAAGGCCGCTTGTGGCCTCCAGCGTGCACTCTTGTTTACGTCATTGTCATCTTCAAGACCA
  GTCCTTTGTGATTAGTTTTGCTTCGCGAGCCCTGGTGTGGACTGTGGTCTGTATGAATCGTGTGTAACTG
  TGGTGAGGGGCTTGTCCTGTATGTGAGTCTGTACCCAGGTGGGGTCTGTGCCCTGCACACCGGGCCCCTC
  TGTATTTATCGCTGCCTGAATGCAACAGTAATTTATATCCAGGACAAATACAGTCTGGGCGTCACTAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001347.2
• Summary: 'The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]'
• Locus ID: 1609