GRIK2 (NM_001166247) Human 3' UTR Clone

CAT#: SC216296

3`UTR clone of glutamate receptor ionotropic kainate 2 (GRIK2) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GRIK2
• Synonyms: EAA4; GLR6; GluK2; GLUK6; GLUR6; MRT6
• ACCN: NM_001166247
• Insert Size: 1797 bp
• Sequence Data:
  Insert Sequence

  >SC216296 3'UTR clone of NM_001166247
  The sequence shown below is from the reference sequence of NM_001166247. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTTGTCATCATCACCATCTTCATCATCATTATCATCATGTTCTTAATAAGTACATCTAATAAGGTCCTTC
  TGTAGTGCCATGGTAGAAGAATTGAGGATGTCCCTGAAGTGCCAGCGTCGGTTAAAACATAAGCCACAGG
  CCCCAGTTATTGTGAAAACAGAAGAAGTTATCAACATGCACACATTTAACGACAGAAGGTTGCCAGGTAA
  AGAAACCATGGCATAAAGCTGGGAGGCCAAACACCCAAGCACAAACTGTCGTCTTTTTCCAAACAATTTA
  GCCAGAATGTTTCCTGTGGAAATATGCAACCTGTGCAAAATAAAATGAGTTACCTCATGCCGCTGTGTCT
  ATGAACTAGAGACTCTGTGATCTAAGCAGTTGCAATGATCAGACTTGATTTACAAGCATCATGGATCAAC
  CAAGTTACACGGGGTTACACTGTTAATCATGGGTTCCTCCCTTCTTCTGAGTGAATGTTAACATGCGCAT
  TTTGTGGCTGATTTCAAATGCAGTCCAGTGAGAAATTACAGGTTCCTTTTGAAGCTCAACTGTTGCCAGG
  AGATGGAATATCAATGCCCAACAGGGCAACCAATAAAAGTGTCACTAAGAATATAAATATTTGGAATCAG
  CAAAAACTGTAGTGTTACAGGAAACAGTACAGTCTTCTGAACACCCAGATCATAGAGGTGATGATGTTAC
  TAGCCCCCAACTACTCAGTATAATTATTGTCTGAATGCAAAGTATGTGTTTATAGGATGTGAAAAAATGT
  AATGCAAAACAAATTTGAATCCCATGGCAGTTGGAATATAAAGCAGATGTTCATCACTTATTTTCCTTTT
  TTCTTTTCTTATTTTTTTTTTTGACAGTCTGTGTCACTGATTGAGATAGAAATGCCAATTATCAAGGAAA
  TAATGTTTTCTTAAGTTCCCTAAGGCAGAAGATTTAACATGCAATTCTACCAGATCCCTTCCTATTCCCC
  CAACACCTTTTCTCTAACCCCCATATCCCAAATAATAATAATAATAATAATAATAATAATAATAATAATA
  ATAAAAGCAGTTGGTTCAGTGATTCTGAATTAAAAGGATAATGTTTTGCAATGTTCAAGTTGTAAAAACT
  GGCCGAGTATTGGCTGTGTGGAAGACTAAAGCTTTCATTCTAACATTCAGACATAGCAATCCAAACCCTT
  GTTCCTGCTGTAAATGAACTTGATGGAGCATGGGCAGATTTCAGTGATACGAGAAAGGGGACTGGTCATC
  TATAGAAAAATCTGTGAGAGAACTTGGAAGTGGACTGCGTTTATCAATACAGTCACAATGTTAAATGAAC
  AAAATTCTTGAACAGTTTTTTTTCAAAAAATGTTCAGGTTTATTTGTGGAAATGCAAGATTTCTATGAAA
  ATAGTTTTTGTATGGAAATTTTTGTAATACTTTTTATCAACAAAACAAGAACATGTGTTCCTGTCAGGGG
  TGTGATGTCAAGCATGAATGGTAGTGCGTGTGCACCACCAACGTTTGGTGAAAACTATTTTTATCAAGAA
  AAAAGGAATCATAGAAGAGAAATATTTTCAAGTTAGATAATATAAAAGCTAGGTGCACTACCACCACTGC
  TTACCATGCCACACCCCTGGTTTCCACGAGGCTGACAACATACTGTAATGAACAATTGTGTGTAAAATGG
  TAAAAGACACAGACCTCTTGACAACATTGTGATAACAGTTGAGTGCACACAGTTTGCTGTTTGAATCCAA
  TGCACAAAATTAAAAAAAATCATTAAAACTATGTTCATTTTACTTTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001166247.1
• Summary: 'Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive cognitive disability. [provided by RefSeq, Jul 2008]'
• Locus ID: 2898