UBE2B (NM_003337) Human 3' UTR Clone

CAT#: SC216319

3`UTR clone of ubiquitin-conjugating enzyme E2B (RAD6 homolog) (UBE2B) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UBE2B
• Synonyms: E2-17kDa; HHR6B; HR6B; RAD6B; UBC2
• ACCN: NM_003337
• Insert Size: 1785
• Sequence Data:
  Insert Sequence

  >SC216319 3'UTR clone of NM_003337
  The sequence shown below is from the reference sequence of NM_003337. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCATTGTTGAACAAAGCTGGAATGATTCATAATAGACAACTGGTCTGTTAATCTTTTTCATCATTGTTGT
  GTATAATTTACCTCTCATTAGAAAGGCTAACAAATTTTAAGTGCCACAGGTTTTAAGGATTCTGCAGAAA
  AAAAAGAAAAAAGTCCTTCAGTTTAGAACCTACAAAAGCTTGTGTATCTTGATTAATGTACTTTTTATTG
  CATGGTGTGAACTAAGTTATTGCTGCATAAATTTGTAATATATCCTGTTTGTATTTTTTTCCAAGTGTAT
  AATGTTGGTGTGGAGTTTTCATGACAGAATATACACATTTTGTAAATCTGTACTTTTTTCAAATATTGAA
  TGCCTTATTTTTGAATTCTTTAGATTTTTAAATTGGAGAAAAGCACTTAAAGTTTTTTATATATGAATAT
  TACATGTAAAGCTGTTAAAATACATAACTTCAGTGCAAGAGACTTTGTCACTTATTTCCTTATGTGTGTA
  GGAGGGGTTAATAAGTCTCTAGCTCTCCATCTATTGATAGTTTCATTTACAATTTCAAAAGAACATTCTT
  ATATTTTATCAAGGAAGTCTTCAAATTTGATTCTAAATAGCGATTATAATCTCCAACTTTATTTTGAATG
  TACCTCTATTAGTTTCAATTGAGTAATTCTAGACATAACTGGTTTGACTCTGTCCAACTCTGTATTTAGG
  CCATTTGTTACAGTTTCTTCATGCATTACTTACTGTTAAAACTGTACCTTTTGCGATTTCACAGTTGGCA
  CTTCTGCCATGAGCAGAGAACTGATGCGACTTGTTTTGCTGCTTGGTAGCACTTTAAAAAATTTTTTGAT
  TAATGAAGAAAGTAAAACCATAAACATTTGCCAAAAATTCATGCCCCAGTATTAGCAATGAATTAGTTGC
  ATTGGTTTGAGAAAGGCACATATTGGAGGGAAATCTTGGTGTAACTTAAATATTTGAAAATTACCTTTAA
  TGCAATGCATATCTGTTTATTCTGGGAAATGTTTTAATGCCAGGGCCTGCTGAGTTGCTTCTTCTTGTGG
  AGATTTTTTTTTTAATCTCCTGAGTTGTATAAAAGTTGTACTGCATCTTAGTTTACTGGATAAATTTAAA
  ACACAGTATTGTAGAAAGCTAATACAAAACTATCCTATGCCTTCAAATAGTATAGAAAATGGAAAATATA
  CAAGTAAATTCTGTTGAACCACCTGTGTAGTCTCTCTAGTAGTTAAAACAGCTATTTTAGTAACCCCAAG
  AGTTTCTTCTCATATCCATAAAATTGGATTACAGTATGGCAATATCTACAGCTTCTATTCATTATGCAAC
  GTTTAACATTGATTGGATAAATGCTTATAGGCTGGGCCAGGTGGCTCCCACCTGTAGTCCCAGCACTTTG
  GGAGGCAAAGGCGGGAGGATCACTTGAGGCCAGGACTTTGAGACCAGCCAGGGCAACATAATAAGACTTT
  TCTCTACTTTAAATTTTTTAAAAAATTAGTTGGGCATAGTGGCACTTGCCTGTAGTCTCAGCTACTTAGG
  AGGCTGAGGTGGCAGAATAGCTTATGCCTAGGAGGTTGAGGCTGCAGTGAGCTGTGATTATGCCATTGCA
  CTCCAGCCTGGGTGACAGAGCAATACCCTGTCTTTAAAAATAAAAGCTATTACTTGGCAAATTCTGAGGT
  AAGTGTATGATCTTAGGGAACTTTGATACAAATGGGAATTCACTCACTGGAATCGTGGCTCCCAAAAAGA
  TGCTTTAATTGTGTGGTGCTTTTATAGTTTAGCTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_003337.2
• Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]
• Locus ID: 7320