Eph receptor B2 (EPHB2) (NM_017449) Human 3' UTR Clone

CAT#: SC216340

3`UTR clone of EPH receptor B2 (EPHB2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: EPHB2
• Synonyms: CAPB; DRT; EK5; EPHT3; ERK; Hek5; PCBC; Tyro5
• ACCN: NM_017449
• Insert Size: 1769 bp
• Sequence Data:
  Insert Sequence

  >SC216340 3'UTR clone of NM_017449
  The sequence shown below is from the reference sequence of NM_017449. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCAGTCTGTGGAGGTTTGACATTCACCTGCCTCGGCTCACCTCTTCCTCCAAGCCCCGCCCCCTCTGCCC
  CACGTGCCGGCCCTCCTGGTGCTCTATCCACTGCAGGGCCAGCCACTCGCCAGGAGGCCACGGGCCACGG
  GAAGAACCAAGCGGTGCCAGCCACGAGACGTCACCAAGAAAACATGCAACTCAAACGACGGAAAAAAAAA
  GGGAATGGGAAAAAAGAAAACAGATCCTGGGAGGGGGCGGGAAATACAAGGAATATTTTTTAAAGAGGAT
  TCTCATAAGGAAAGCAATGACTGTTCTTGCGGGGGATAAAAAAGGGCTTGGGAGATTCATGCGATGTGTC
  CAATCGGAGACAAAAGCAGTTTCTCTCCAACTCCCTCTGGGAAGGTGACCTGGCCAGAGCCAAGAAACAC
  TTTCAGAAAAACAAATGTGAAGGGGAGAGACAGGGGCCGCCCTTGGCTCCTGTCCCTGCTGCTCCTCTAG
  GCCTCACTCAACAACCAAGCGCCTGGAGGACGGGACAGATGGACAGACAGCCACCCTGAGAACCCCTCTG
  GGAAAATCTATTCCTGCCACCACTGGGCAAACAGAAGAATTTTTCTGTCTTTGGAGAGTATTTTAGAAAC
  TCCAATGAAAGACACTGTTTCTCCTGTTGGCTCACAGGGCTGAAAGGGGCTTTTGTCCTCCTGGGTCAGG
  GAGAACGCGGGGACCCCAGAAAGGTCAGCCTTCCTGAGGATGGGCAACCCCCAGGTCTGCAGCTCCAGGT
  ACATATCACGCGCACAGCCTGGCAGCCTGGCCCTCCTGGTGCCCACTCCCGCCAGCCCCTGCCTCGAGGA
  CTGATACTGCAGTGACTGCCGTCAGCTCCGACTGCCGCTGAGAAGGGTTGATCCTGCATCTGGGTTTGTT
  TACAGCAATTCCTGGACTCGGGGGTATTTTGGTCACAGGGTGGTTTTGGTTTAGGGGGTTTGTTTGTTGG
  GTTGTTTTTTGTTTTTTGGTTTTTTTTAATGACAATGAAGTGACACTTTGACATTTCCTACCTTTTGAGG
  ACTTGATCCTTCTCCAGGAAGAAGGTGCTTTCTGCTTACTGACTTAGGCAATACACCAAGGGCGAGATTT
  TATATGCACATTTCTGGATTTTTTTATACGGTTTTCATTGACACTCTTCCCTCCTCCCACCTGCCACCAG
  GCCTCACCAAAGCCCACTGCCATGGGGCCATCTGGGCCATTCAGAGACTGGAGTGAGATTTGGGTGTGGA
  GGGGGAGGCGCCAAGGTGGAGGAGCTTCCCACTCCAGGACTGTTGATGAAAGGGACAGATTGAGGAGGAA
  GTGGGCTCTGAGGCTGCAGGGCTGGAAGTCCTTGCCCACTTCCCACTCTCCTGCCCCAATCTATCTAGTA
  CTTCCCAGGCAAATAGGCCCCTTTGAGGCTCCTGAGTGCCCTCAGATGGTCAAAACCCAGTTTTCCCTCT
  GGGAGCCTAAACCAGGCTGCATCGGAGGCCAGGACCCGGATCATTCACTGTGATACCCTGCCCTCCAGAG
  GGTGCGCTCAGAGACACGGGCAAGCATGCCTCTTCCCTTCCCTGGAGAGAAAGTGTGTGATTTCTCTCCC
  ACCTCCTTCCCCCCACCAGACCTTTGCTGGGCCTAAAGGTCTTGGCCATGGGGACGCCCTCAGTCTAGGG
  ATCTGGCCACAGACTCCCTCCTGTGAACCAACACAGACACCCAAGCAGAGCAATCAGTTAGTGAATTGAA
  TGGAAATAAACGCTTTAGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_017449.3
• Summary: 'This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]'
• Locus ID: 2048