CDC25A (NM_001789) Human 3' UTR Clone

CAT#: SC216410

3`UTR clone of cell division cycle 25 homolog A (S. pombe) (CDC25A) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CDC25A
• Synonyms: CDC25A2
• ACCN: NM_001789
• Insert Size: 1770
• Sequence Data:
  Insert Sequence

  >SC216410 3'UTR clone of NM_001789
  The sequence shown below is from the reference sequence of NM_001789. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTACAGTCGTCTGAAGAAGCTCTGAGGGCGGCAGGACCAGCCAGCAGCAGCCCAAGCTTCCCTCCATCCC
  CCTTTACCCTCTTTGCTGCAGAGAAACTTAAGCAAAGGGGACAGCTGTGTGACATTTGGAGAGGGGGCCT
  GGGACTTCCATGCCTTAAACCTACCTCCCACACTCCCAAGGTTGGAGCCCAGGGCATCTTGCTGGCTACG
  CCTCTTCTGTCCCTGTTAGACGTCCTCCGTCCATATCAGAACTGTGCCACAATGCAGTTCTGAGCACCGT
  GTCAAGCTGCTCTGAGCCACAGTGGGATGAACCAGCCGGGGCCTTATCGGGCTCCAGCCATCTCATGAGG
  GGAGAGGAGACGGAGGGGAGTAGAGAAGTTACACAGAAATGCTGCTGGCCAAATAGCAAAGACAACCTGG
  GAAGGAAAGGTCTTTGTGGGATAATCCATATGTTTAATTTATTCAACTTCATCAATCACTTTATTTTATT
  TTTTTTTCTAACTCCTGGAGACTTATTTTACTGCTTCATTAGGTTGAAATACTGCCATTCTAGGTAGGGT
  TTTATTATCCCAGGGACTACCTCGGCTTTTAATTTAAAAAAAAAAAAGAAGTGGGTAAGAAAATGCAAAC
  CTGTTATAAGTTATCGGACAGAAAGCTAGGTGCTCTGTCACCCCCAGGAGGCGCTGTGGTACTGGGGCTG
  CTGCTATTTAAGCCAAGAACTGAGGTCCTGGTGAGAGCGTTGGACCCAGGCTTGGCTGCCTGACATAAGC
  TAAATCTCCCAGACCCACCACTGGCTACCGATATCTATTTGGTGGGAGGTGTGGCCCTGTTCTTCCTCAC
  CCCAGTTCCATGACATTGGCTGGTATAGGAGCCACAGTCAGGAAAGCACTTGAGGCAGCATCTGTTGGGC
  CACCCCCGGCTCAGTGCTGGAATGTTGCAGTGTAGGTTTCCCAGGGAAGGGGGGTGGGGGTAGGTGGGCT
  CCACAGGATGGGGGAGGAGCATGTCCACTGAGTATCTTCCTTATGTTGCTGTGATATTGATAGCTTTTAT
  TTTCTAATTTTTAAAAAATGGTCATATTATGAGTCAAAGAGTATCAAATCAGTGTTGGATGGACCACCCA
  AGGGTGAGGAGAGGGGCTGGAAGCCCTGGGCATTAGGAGAAGGGAGTGGGTGCTGGCATGGACATGACTG
  GATAGAATTTTCTCAGGAGGGAGCTTGGTGGATTTTGAAGGTAAAACTTTCTGGGTTTATCATGTTTTAA
  TTTTAGAGACAGGGAGTGATGAATCATCACCGGTTGTCCCCTTATCTAACTCCATAAAAGTGGGAATTTC
  AAAAGAACACCTCATCCAAGGAGCTGGGGCAGACTTCATTGATTCTAGAGAGACCTGTTTCAGTGCCTAC
  TCATCCCTGCCCTCTGGTGCCAGCCTCCTTACCATCACGGCTTCACTGAGGTGTAGGTGGGTTTTTCTTA
  AACAGGAGACAGTCTCTCCCCTCTTACCTCAACTTCTTGGGGTGGGAATCAGTGATACTGGAGATGGCTA
  GTTGCTGTGTTACGGGTTTGAGTTACATTTGGCTATAAAACAATCTTGTTGGGAAAAATGTGGGGGAGAG
  GACTTCTTCCTACACGCGCATTGAGACAGATTCCAACTGGTTAATGATATTGTTTGTAAGAAAGAGATTC
  TGTTGGTTGACTGCCTAAAGAGAAAGGTGGGATGGCCTTCAGATTATACCAGCTTAGCTAGCATTACTAA
  CCAACTGTTGGAAGCTCTGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001789.2
• Summary: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 993