UBE2H (NM_003344) Human 3' UTR Clone

CAT#: SC216420

3`UTR clone of ubiquitin-conjugating enzyme E2H (UBC8 homolog yeast) (UBE2H) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UBE2H
• Synonyms: E2-20K; GID3; UBC8; UBCH; UBCH2
• ACCN: NM_003344
• Insert Size: 1796 bp
• Sequence Data:
  Insert Sequence

  >SC216420 3'UTR clone of NM_003344
  The sequence shown below is from the reference sequence of NM_003344. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACTTTTCCGAAGATGAGGCCCAGGATATGGAGTTGTAGTAGAAAAAGCACCTGCTTTTCAGAAAGACTA
  TTATTTCCTAACCATGAGAAGCAGACTATAATATTCATATTTAAACAAAGCAATTTTTTTTATTACTAAA
  CAAGGTTTTTATGAATAATAGCATTGATATATATATATTATATATCACCCTTTAGATCTTGATTTCTTGG
  TCATTTCTCAACCTGAGGTGCATAGCATATTCCCACATTCCATTTGGTAGCAATATGCGGTCTGAATGCA
  TGCATTCATGAGTCCATGTGGCCAAGTCAGCCTGTGTGCTACTGAACTGTCGAAGGAAATAGCCGCTCTG
  ATAGGTAGATGTGAGTAAAAAGAACAGGAAAAAATTGCTTCTTTTATTGGTTTCCAAAGAAACAAACCAA
  ACCAACCAGCTCTTGGATGTGAAGATAAAATAGTGCTTTTTTGAAATGGAGAGGAAAAACTTGGGGAGGA
  AGAGGCCTGCTGTGGGGGCATCGGAGCCAGCCATGTAAGAATCAGAGCTGCTCCTTCCTGTGAATCCTAG
  GTGGCCCTATGTCTTCTGTGGAGTTACAGTATAAAGCAGGGAGCTAATTAAGAGTATTAAAACTTAAAAC
  CATTTTTTGACTCTGATTTTAAGTACATTTTTATATGTCAGTTGCTGCCCTTCACACTACCAGGCCCTGC
  AGCCACAGTGTTCTGTTGGAGAAACTTGGGGAAGTGTTTTCTGAACCAGTTCTTTTTCTTGGGGTAGAGC
  GTGAAATCCAGACCTGTTTTTGAAAGGACAGCACAGGAGGAGAAAAGTGACTGGGACGATGCTTCCTCTC
  ATCCAAAACACATGCAGAGTCACATCCTCATCCTAGTGTTTGGCAGTTTGAGACCGCTACCCTGAACTTA
  AGAGCTTTAAATATGAGGGTTGTGTTTCTGGGGGGGTTATTTTTTTGGTGTGTGTGTGTGTGTATTGTGC
  TTAGAAAGGTTGCAGATTTCATCTTCACCTACCAAGAGGCGGCCGCCTCCTTGGACAAGCCGCTCATGGA
  CCATTAGACTTGAGAAGACGACCCACGTGGTGACTGCAAAGCCCTGGGTACTTGCCTTCATGTTCTGGTT
  TGGTTTTCCCTTTTTCTGCTGCTCTTGGCAGCAGCCTGGTCATCATCTGCTTGTGGGAGTGGGAAATGGG
  TATTTTAGACCCAACACAGGTTCTAAATTTAAAACAAATAATAATTGGAACAAATAAAGGTTCTTGTACC
  CAGAGTGATTCTACATGGGAAAAACAAACCAGCTTTTTTCAGAGAGTGACCGAATAGGAAGAAGGGATTC
  AGAAATATGATGTAGTAGAACATGCCCTGCAGGAAGAAAATGTTTTCTCTCCTGCGTATGTTAAGAGGAA
  ATCAGTTTAAAATGCCTGCTAATCAAATGTCGACTCAGCTAACCATATGTCCAGGTTGCTGCAGAGTTTC
  TTTCAAATCTGAGTGTTGCCTGCTGAACTCGGAGGGGAGTTTGGAGAAATTTTAGACAGCAGCTCTGTGT
  TCTTTTTTTGCCGCAGCCAATAAGATAATAGCAGAATTAGCCAATCCTAAAACCTTCTAAAGACGATTTT
  TGAGCATCCCACTGGGATTATTTAATTTTTCTTTTTGACGTGTGTTTTTAGTTTAGAAGTGCTGTATTCC
  ACAAGGTTTAAGTCAGTGAGGTTGGAAGATACCCTATCTTTTTTATTCAAAAAGCACAATCAATCTTTTG
  TTTGAAAATCTATATAAAGTACAACTTGCTTTTAGCATGATTATTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003344.2
• Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]'
• Locus ID: 7328