PAPOLG (NM_022894) Human 3' UTR Clone

CAT#: SC216566

3`UTR clone of poly(A) polymerase gamma (PAPOLG) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PAPOLG
• Synonyms: FLJ11805; FLJ12972; FLJ13482; FLJ14187; MGC133307; MGC133308
• ACCN: NM_022894
• Insert Size: 1812
• Sequence Data:
  Insert Sequence

  >SC216566 3'UTR clone of NM_022894
  The sequence shown below is from the reference sequence of NM_022894. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TTCGACTGACCCTTAATCGGTAAAAGCAGTGCCTCCTCACTTAAGTGAACAAGCAATCATTTAGTGGCAT
  AGATGCAGCCACTTGTTTTTTAAATAGAAGTGGCTGTCATACGTGAAATAAGGTGAAAGTGACAGCCTTC
  AGTCTAATAACCTTGAAGTGGTTTTTGAACTGTCAAACTTTGACCTGTAGATGCTGTAGCATTCTCTCAC
  TGATTGCTACATACACTTCTCTGAGGATCACCTGCTGTCAAATTGCCATCTACAGTATTACAGCTTTGCA
  TTTGGGGGTTTTACTGCCTTAACTTTCAATGCTTGTTATGGGAACCAGTTCTTAGCCACTTGGACACTGA
  TAACAAGTCCTGAACTCCTTTTTTGTTTTGTTTTTTGTGTTTTTCTTTTTTTGTCTTATGTTGTAATCAT
  TGTATAGAGCTAAAAAAATTGAAAACAAACAAAAAAATTGTCTTGTATTTTCCAAATGTTAATACATTTA
  CTTTAGCATTGAAGCCACTTTGAAACCTGAGATAAATGAATGTGAGGTATCTTTTCTGCTTTCCTCATTT
  GTGTAGATGTACTGTCTGTTCTGTTGATTTAAATTATTTTTTTCCAGATTGGCACATGAAATATTTAAAC
  TTTTTTGTGTGCCTTTCTGTCCAAATGTTGCATAGTTACCAGGGAAGATTAGTCCAGTGATTACATAAGA
  GTTGGGCACCATAAATTCTCTATATTTTGCCTCCCATGGAGGCCTTTGAAATGCATCTTTATTAAAAATC
  AAAATATAACCAGGATACTGAAAGTCAGTATATGAATGGTAAAATTGTTACATATCCTATTTCATGCCAT
  TCTTGTTAGTTGACTGGTATTTTTTACTGAGGAGCAACTCATTCCAGCATCAACAATAAGATAACCTTTA
  AGTATGGCACACTTGTATTTTTGAGGTGTAAAATTAACTTGGCATGATAATTCCTGATCATTATTTACCC
  CACAACTTCAAATAGTTTCTTCACGGACTAGGCATGCAGAAATAAGCAGTGGATTTTATTGAAACCTAAA
  GGCATTTTGAATGACATTGTTACCAACCATTAATTGGCTCAGGACCTTTGTAATTTTTATTTAACTATAT
  GAGTTGTCTTTTTTTACGCTGCTTTTTTCAATGCATTTCTTAATATTTTTTAAGTTTCATGAATGCATGC
  TCAGTTTATTAAAATCCATAACCATGTAATTCTTGTAATATGTTGATTCAGTGTTTTGTAAATGAAGTCG
  TATGTATTTTCAGAGTATTTTTGTATGTACTGTAAGATACCATCTTTTCAAAGAGAAACGTTTAAAACCT
  TTATTGTCTCTCCTTAGTCTAATTTTTTAAATATGGATTATGCTTGAATTACTATATTTAAAATGAAAAT
  GTATAGATTACACAGCCAGGAATGCTAGTATCTACCACCTTCTTTAGATTTCACTCAGCATTTAGTAGGT
  CCAGTAGGAAAAACCCAAAATGGTACATTGAGTAGTGTGGGGAAGACACCTCGTGTATTATAGGACTCAC
  GGGATGCCATCTAGCAAGTGGAGAAATAGCAGTCCATTTTCTCTTGCAAGCCCTTTACCATTGAGTTGAA
  ATTCTTGAATTGCCAGAGAGCCGTATCCTCCAGGTCCCACTCCTGTTCATTGCCCCTTAGGTCTCCAAAA
  TGTAAATTCTAATGTTTACCTTGTACCATGGAAAACATGAAAAGAGTCTTAGAAGTAAAGAACAACAAGG
  AAAAAAATGTTTGTTCTATTATTCCCTTTTTATATAGCAGCAAAAGATAAGAATAAAATCAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_022894.2
• Summary: This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. [provided by RefSeq, Jul 2008]
• Locus ID: 64895