SIRT1 (NM_012238) Human 3' UTR Clone

CAT#: SC216574

3`UTR clone of sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) (SIRT1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SIRT1
• Synonyms: SIR2; SIR2alpha; SIR2L1
• ACCN: NM_012238
• Insert Size: 1833
• Sequence Data:
  Insert Sequence

  >SC216574 3'UTR clone of NM_012238
  The sequence shown below is from the reference sequence of NM_012238. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGGAAGTAACAGACATGAACTATCCATCAAACAAATCATAGTGTAATAATTGTGCAGGTACAGGAATTG
  TTCCACCAGCATTAGGAACTTTAGCATGTCAAAATGAATGTTTACTTGTGAACTCGATAGAGCAAGGAAA
  CCAGAAAGGTGTAATATTTATAGGTTGGTAAAATAGATTGTTTTTCATGGATAATTTTTAACTTCATTAT
  TTCTGTACTTGTACAAACTCAACACTAACTTTTTTTTTTTTAAAAAAAAAAAGGTACTAAGTATCTTCAA
  TCAGCTGTTGGTCAAGACTAACTTTCTTTTAAAGGTTCATTTGTATGATAAATTCATATGTGTATATATA
  ATTTTTTTTGTTTTGTCTAGTGAGTTTCAACATTTTTAAAGTTTTCAAAAAGCCATCGGAATGTTAAATT
  AATGTAAAGGGAACAGCTAATCTAGACCAAAGAATGGTATTTTCACTTTTCTTTGTAACATTGAATGGTT
  TGAAGTACTCAAAATCTGTTACGCTAAACTTTTGATTCTTTAACACAATTATTTTTAAACACTGGCATTT
  TCCAAAACTGTGGCAGCTAACTTTTTAAAATCTCAAATGACATGCAGTGTGAGTAGAAGGAAGTCAACAA
  TATGTGGGGAGAGCACTCGGTTGTCTTTACTTTTAAAAGTAATACTTGGTGCTAAGAATTTCAGGATTAT
  TGTATTTACGTTCAAATGAAGATGGCTTTTGTACTTCCTGTGGACATGTAGTAATGTCTATATTGGCTCA
  TAAAACTAACCTGAAAAACAAATAAATGCTTTGGAAATGTTTCAGTTGCTTTAGAAACATTAGTGCCTGC
  CTGGATCCCCTTAGTTTTGAAATATTTGCCATTGTTGTTTAAATACCTATCACTGTGGTAGAGCTTGCAT
  TGATCTTTTCCACAAGTATTAAACTGCCAAAATGTGAATATGCAAAGCCTTTCTGAATCTATAATAATGG
  TACTTCTACTGGGGAGAGTGTAATATTTTGGACTGCTGTTTTCCATTAATGAGGAGAGCAACAGGCCCCT
  GATTATACAGTTCCAAAGTAATAAGATGTTAATTGTAATTCAGCCAGAAAGTACATGTCTCCCATTGGGA
  GGATTTGGTGTTAAATACCAAACTGCTAGCCCTAGTATTATGGAGATGAACATGATGATGTAACTTGTAA
  TAGCAGAATAGTTAATGAATGAAACTAGTTCTTATAATTTATCTTTATTTAAAAGCTTAGCCTGCCTTAA
  AACTAGAGATCAACTTTCTCAGCTGCAAAAGCTTCTAGTCTTTCAAGAAGTTCATACTTTATGAAATTGC
  ACAGTAAGCATTTATTTTTCAGACCATTTTTGAACATCACTCCTAAATTAATAAAGTATTCCTCTGTTGC
  TTTAGTATTTATTACAATAAAAAGGGTTTGAAATATAGCTGTTCTTTATGCATAAAACACCCAGCTAGGA
  CCATTACTGCCAGAGAAAAAAATCGTATTGAATGGCCATTTCCCTACTTATAAGATGTCTCAATCTGAAT
  TTATTTGGCTACACTAAAGAATGCAGTATATTTAGTTTTCCATTTGCATGATGTTTGTGTGCTATAGATG
  ATATTTTAAATTGAAAAGTTTGTTTTAAATTATTTTTACAGTGAAGACTGTTTTCAGCTCTTTTTATATT
  GTACATAGTCTTTTATGTAATTTACTGGCATATGTTTTGTAGACTGTTTAATGACTGGATATCTTCCTTC
  AACTTTTGAAATACAAAACCAGTGTTTTTTACTTGTACACTGTTTTAAAGTCTATTAAAATTGTCATTTG
  ACTTTTTTCTGTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_012238.4
• Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
• Locus ID: 23411