WWP2 (NM_199424) Human 3' UTR Clone

CAT#: SC216628

3`UTR clone of WW domain containing E3 ubiquitin protein ligase 2 (WWP2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: WWP2
• Synonyms: AIP2; WWp2-like
• ACCN: NM_199424
• Insert Size: 1819
• Sequence Data:
  Insert Sequence

  >SC216628 3'UTR clone of NM_199424
  The sequence shown below is from the reference sequence of NM_199424. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGCTGTATGCCATTGAGGAGACCGAGGGCTTTGGACAGGAGTAACCGAGGCCGCCCCTCCCACGCCCCC
  CAGCGCACATGTAGTCCTGAGTCCTCCCTGCCTGAGAGGCCACTGGCCCCGCAGCCCTTGGGAGGCCCCC
  GTGGATGTGGCCCTGTGTGGGACCACACTGTCATCTCGCTGCTGGCAGAAAAGCCTGATCCCAGGAGGCC
  CTGCAGTTCCCCCGACCCGCGGATGGCAGTCTGGAATAAAGCCCCCTAGTTGCCTTTGGCCCCACCTTTG
  CAAAGTTCCAGAGGGCTGACCCTCTCTGCAAAACTCTCCCCTGTCCTCTAGACCCCACCCTGGGTGTATG
  TGAGTGTGCAAGGGAAGGTGTTGCATCCCCAGGGGCTGCCGCAGAGGCCGGAGACCTCCTGGACTAGTTC
  GGCGAGGAGACTGGCCACTGGGGGTGGCTGTTCGGGACTGAGAGCGCCAAGGGTCTTTGCCAGCAAAGGA
  GGTTCTGCCTGTAATTGAGCCTCTCTGATGATGGAGATGAAGTGAAGGTCTGAGGGAGCGGGCCCTGGGG
  CGAGGCCATCTCTGCCTGCCTCCCTAGCAGGCGCCAGCGGTGGAGGCTGAGTCGCAGGACACATGCCGGC
  CAGTTAATTCATTCTCAGCCAATGAAGGTTTGTCTAAGCTGCCTGGGTATCCACGGGACAAAAACAGCAA
  ACTCCCTCAGACTTTGTCCATGTATAAACTTGAAGTGGTTGTGTTGTAGGGTTGCAGGTTTTTTGTTACG
  CTGCTGTCACTTTCTGTCCAGGAGCTGGCACCCCAGGTGTTCTGAGACCTTGAGGGACCCAGACCTTTGG
  GTCCAAGAGTTTCCCAAACAGCCACGCCTCTCAGGAACCCACCTGGCGGTTCCGTGAGCTCAGGCAGGCC
  TGACCCGGCGGCACAGCCTGGCAGGGACCTCGTCCCCAAGCCTGGCAGAATGAGAGGGGTTGAGGTCCCG
  AGCGCCACTCCTAGCCTTGCCGCCTTCAATAGAGAAGAAATCCCTTTGCTAGATAGGGTCCCCCAGGCAG
  TCCCCAGTGGCGGGACACAGGGGTCCGGCTGTGGAGCTCCCCTGCCAGCCCCTGGAGCTCCAGGAGGGCC
  TGTTGGTCCCCTGTTCAGAATGGAGTGCAGCCCGCCAGCGGAAAGTGTTCATTCTGCATAGGTGTGAGGC
  TTTATCTGCACACAGGACATGAAAACCAGCAGAAAGGCCCTGAGCTGCTGCATAGCCCCATCTGATTTCT
  GCAGTTCCCGCCAGCCTCCAACACGGGGACTCTGCCGTAACTGGAATCTTCATAGGTCATATTGAAATCT
  TCAAGGTGACCATGCCCCACCGGGGTGCTGGGGCAGTAGTCATGGCAGACTCCCGGCCTGGGCCCCCAGG
  ATTCTAGGACCCCCAGGCAGCCCCTTGGACTGGTCCCGGGTGCCTTCCAAGCACAGTCTCCATGCTCCCA
  GATTCTCGACCTTCCCCCGGCCCGGGAGGTGCAGCCTGCGTCTGCCTCTGTCGTGTGTGCTGATTTGAGT
  GGCTTAGCTTGCCACAGCGCAGCCTCTTCTGTCCCTTTCAGTCATTTGCTGTACTTCCCTGTGGCACGTT
  ACCATGGAAGCCGCTCCAGGGTGGGTCAGGGTGCAAGCTGCTGGTGAGGTTTGGAAGCATCAGGCTCACG
  GGTGTTCATGTGTGTTCGTGCGTGTGTGTGCGTACGTGTATATAACTGAAGTGTCTGTACGGAATGCCCT
  TTGCTAGCCATGGGCTGGTCACCAGATTGTTTTGTAATGCCCGCCCCTTGCCTCGATATTGCCAGTTTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_199424.1
• Summary: This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10. [provided by RefSeq, Jul 2012]
• Locus ID: 11060