CRNKL1 (NM_016652) Human 3' UTR Clone

CAT#: SC216652

3`UTR clone of crooked neck pre-mRNA splicing factor-like 1 (Drosophila) (CRNKL1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CRNKL1
• Synonyms: CLF; Clf1; CRN; HCRN; MSTP021; SYF3
• ACCN: NM_016652
• Insert Size: 1848
• Sequence Data:
  Insert Sequence

  >SC216652 3'UTR clone of NM_016652
  The sequence shown below is from the reference sequence of NM_016652. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATGCTGAGCACCATCCAGATGAGGACGTCGATGAGAGTGAATCCTGATCTTTTTTTCATAGACAAATGTT
  TTGTTATTTTTATAAATTAATTGTTTGGAACTCTTGTGACTCCTGGAAGTTCTTATATATTTCACCAGTA
  AGAAATTGATTGGTATCTTTGATGGCTACTTTTTAAGTTATTTTTTAAATGCTCTTGGGTTAGCTAGGGG
  TAGGGATTGCAAGTAAAGGACTTTTTTAACTGCTGGATTTGTTTTTCCAACTGAGTCCAAACTTTTCTAA
  TGTCTGTCCACATCATGCATTAGGAAATGTAATTAAGGTAACATTCTACAGTTACTTTTCATGTCATACC
  CATAAAGATAGTTTATGCATTCATCTGAAATGTGTAACTTTTTCATGTCTTCAGAGTCACAGACTTGAGT
  TCATTTCCCAGCTCTGCCACTCATGATTATATAACTTAATTTTCATTTTCCTCATTCACAAAATGGGCCA
  ATAGTTTGACAGCTCATTTTGAAGATTACATTATAAAAGGAATATACCTGGTGGGTGCATAGTAAGTGCT
  CAGTAAATTGTTTGTTCTAAGCCACTTTTAAAAATGGTTTCATTCCTTGTAGAATTGAATGCGAGTGGAT
  TAATAATTTACCTTACTTTCTTACTAGTGTCCAGTTATATTGTTTTTTAGAACAACACTTGGAAAATAAT
  TTGCAGTGATTATATTTCTGAACAAGGTTCAGAAAACATTGTTTACTAAGAATTTAGTCTAATAATTTCA
  GTTAGGCGCTCATCAGTTCTCCAGAGTGGTTGAGTTTGTAATACCTTGTTTAAAGAATAATGGCTTGTTC
  ACGTGTGTGCTATGAAAAATGATGTCCCATGTTCACATAAATTTGGGAAATTCTGGACTAAGACTTAAGT
  CTCGTTAATCAAATCTCTTTATAGTTAGGCTTCTGTACATTATGTATCTCCAGTAGCAATGTTGCCATAT
  TATTTATTTCCCAAACTTAGTGGACAATGGAGTCATTTCTACCTAGAGTACCAGTAAACATCTCCCAGTG
  TGCTATAGTAGAAAATGTCTACTCCTCACTGCTGACATGTTAAACTTACTCTTGGTTTAGAGCATGTGTA
  GAAACACCTAAGGTAGCTCTATGCTAAATAATGAAGAGTAGCACAAGAATGAATGTATTTGCTGATACGT
  TGCTCACATTCTCAAGCAAAAATTCAACTGCATTAACCGATCTGAGAGTTTTCCTTTAACCTGGACTGTG
  TTTCTCAAGCACATTTTTTCTTTGTTCACTGCCCAAGGACTAGAACTGTATTTTTAAGGTTGTTTTCCCC
  TAAAAGGACCTTTAGTAAGCAAATTTATTATTAAATGTGCACATCTTATTCACCCAAGGGAATAAAAGCT
  ACTTCGTAATGTTGTTACTAAATTTTATCTTGAAAATAAATAACAGTGTTTGAGGACAGAAGAAACTAGG
  ACTGTTGTTGAGATAAAATCTGTTTTCAGCCTGACTGCTTTAGTCACTGGACGAGCATCAGCTACTGCGG
  TGTCAGATCACTTCTCGAGTAGAGGTCTGCCTAGTGAGTGAGCAGGAGAACATCATCTTAATAGCCTTTG
  GCTCACCCAGAGTCAGGGAGAGATGAAAATAATAGGTCAGAATGCAAGTGACTTAATCACAGTCTTCCTA
  GAATTATCTGTTTATGTTGCATGAATGCATAAGAAAAAAATTCTGTCATGTTCCCAAGGTAGAATTGTAA
  TAAGCATGTGCCAGTGTGAAAGCTGGAAGGAAAAAAGCTATTCTCTAACAGGAGAACATAAAGATTCATT
  TCTCTCCCACCCTCAACTCCACCAAAGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_016652.4
• Summary: The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013]
• Locus ID: 51340