POLR2D (NM_004805) Human 3' UTR Clone

CAT#: SC216754

3`UTR clone of polymerase (RNA) II (DNA directed) polypeptide D (POLR2D) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POLR2D
• Synonyms: HSRBP4; HSRPB4; RBP4; RPB16
• ACCN: NM_004805
• Insert Size: 1854 bp
• Sequence Data:
  Insert Sequence

  >SC216754 3'UTR clone of NM_004805
  The sequence shown below is from the reference sequence of NM_004805. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TATCCAGACAAAGCGCAGCTTTCAGTATTAATCTCCAAACATCACTGCTGCTCGGAGAAACCACATCCCC
  AGGCATAACACCACCTTCCCACTGTCTGGGGCTGACTTGCACAGAAATTCTGTTGAAGACAGTTGAGAAT
  TCCTTTGGAGAAAACAGCCCAGCTTGGCGTGGGGTTAGGTTGCTGTTTCAAATAACTCACAGGCCCAGGT
  GACATGGAATCTTGGAGCAGCCTTGTGCAGTGGCAGCCAGTGGCTTCCTGAACGTGCCTCTGCGAAGTGT
  GAGATGAGGGGTCACATAACCACACTGTTGACTACCTCATTCCTGGTTTTTGGCCTCCACATCATCTTTT
  TTCTTAATATTTCATGTTTTAATTTCAGGGTGTTTATACTTTTTGAAACTAGACCAGAAGATAGTAGACT
  TTATAGAGAAAGACCAGTTTTACCTAGATACTAAAGGAAGAATTAAACCGCTGTTAGTTTGAAATGCTTT
  TTTTTTTTTTTTTTAAATGGAGATAGGGTCTTAACTCTTGTCCAGGCTGGAGGAGTGCAGTCGTACAGTC
  ATGGCTCACTGAAGTCTTGACCCCGCTGCCTCAGCCTCCCAAATAACTGGGGCCACAGGTGTGCACCACA
  ACTCTCAGCTAATTTTTAAAATTTTTTATAGAGGTGGGGTTTTACTATGCTGTCCAGACTGGTCTTAAAC
  TCCTGGGCTCAAGTGATCCCCCTGCCTTGGCCTCCCAAACTGGTGAGATTACAGGCATGAGCCACCACAA
  CTGGCCTGAAATTCTTAAAGGATGGGAGTGTCGATGACAGCACCTTGGCATCGTTGTGCCTAACCTGGGA
  GACGGAAGAAGCACGCCATGGGAAGTGTTTACACTTGGGGACAAGTGCTAAGTATTGTGGAGCCCATAGC
  CCCTTGAGATAGATGGCTACTTTGCCTTTCTTCTTGAACTGTCTTGCAGAATGTGGATTTGGGGTAAGTG
  GTCTTGAAGGATTCATTTAGTCACCCTCAAATTAAGATTTTTACTTCATCTTTCTTGGGCCTGCACCTCC
  AAGATAACAAAGAAGAAGCAATGGTCGTGCCAAAGAGGTCCACAACCAGGTGTGCACTGTTCACTGCAGC
  CCATTTGCTGTATGAACTGTGGTTGTTGTGTGCCCAATGACAAGGCTACTAAGAAATTCATCATTTGAAA
  CGTAGAGGCCGCAGCAGTCAGCGATGTTTCTGAAATGAGCATCCTTGACGCCTGTGTACTTCCCAGGCTG
  GATGTGAAGCTACATTACCATGTGAGTTGTGCCATTCACAGCACAGTGGTGAGGAATTGAGCTCATGAAG
  CAGGCAAGGACCGAACACCTCCACCCCAACGTAGACCTGCAGGTGCTGCCCCATGACCTCCACCAAAGCC
  CATATAAGGAGCGGAGTTGTTAAGGACTGAAGAAAAACTTCTCTGGAGAAAAATAAAATTGCAATTCTAC
  TTAAAAAAATTTTTTTTTTTTTTTACTTCATAGGCCAGGCTTGAAGTTCTGAACACTTTGAAGTCTCCAA
  TTATGAGAGATCCAGTCTAAGCCTCTGGCCTGCTAATTAGCAATAAGTGCTTTATTTGGAAGGAGGGAGT
  CATCCACTCTTGAGCCACTGCAGTGAAGTCACTTGATCTCAGTCTGGGGGAAAACACTTCAATAGCTAAA
  CATTCTAGCTTTGATTTTTCTGAAGGGAATACACTTGTTTTCAATTTTGGGGTTTTTCTTTGGGGCACTT
  GCTTGACTCTGTATGAACTTGTGATCCAAGGAAAAAGGAGAAAGAACAGTGTTGGCTTTTAAAATCAGGA
  TGGTTTTATGTTTGCTACGAAATAAGGCAAGAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004805.3
• Summary: 'This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 5433