IL10RA (NM_001558) Human 3' UTR Clone

CAT#: SC216768

3`UTR clone of interleukin 10 receptor alpha (IL10RA) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: IL10RA
• Synonyms: CD210; CD210a; CDW210A; HIL-10R; IL-10R1; IL10R
• ACCN: NM_001558
• Insert Size: 1868 bp
• Sequence Data:
  Insert Sequence

  >SC216768 3'UTR clone of NM_001558
  The sequence shown below is from the reference sequence of NM_001558. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCTCATCTCTAGCCTGCAGTCAAGTGAGTGACTCGGGCTGAGAGGCTGCTTTTGATTTTAGCCATGCCTG
  CTCCTCTGCCTGGACCAGGAGGAGGGCCCCTGGGGCAGAAGTTAGGCACGAGGCAGTCTGGGCACTTTTC
  TGCAAGTCCACTGGGGCTGGCCCCAGCCAGGCCCTGCAGGGCTGGTCAGGGTGTCTGGGGCAGGAGGAGG
  CCAACTCACTGAACTAGTGCAGGGTATGTGGGTGGCACTGACCTGTTCTGTTGACTGGGGCCCTGCAGAC
  TCTGGCAGAGCTGAGAAGGGCAGGGACCTTCTCCCTCCTAGGAACTCTTTCCTGTATCATAAAGGATTAT
  TTGCTCAGGGGAACCATGGGGCTTTCTGGAGTTGTGGTGAGGCCACCAGGCTGAAGTCAGCTCAGACCCA
  GACCTCCCTGCTTAGGCCACTCGAGCATCAGAGCTTCCAGCAGGAGGAAGGGCTGTAGGAATGGAAGCTT
  CAGGGCCTTGCTGCTGGGGTCATTTTTAGGGGAAAAAGGAGGATATGATGGTCACATGGGGAACCTCCCC
  TCATCGGGCCTCTGGGGCAGGAAGCTTGTCACTGGAAGATCTTAAGGTATATATTTTCTGGACACTCAAA
  CACATCATAATGGATTCACTGAGGGGAGACAAAGGGAGCCGAGACCCTGGATGGGGCTTCCAGCTCAGAA
  CCCATCCCTCTGGTGGGTACCTCTGGCACCCATCTGCAAATATCTCCCTCTCTCCAACAAATGGAGTAGC
  ATCCCCCTGGGGCACTTGCTGAGGCCAAGCCACTCACATCCTCACTTTGCTGCCCCACCATCTTGCTGAC
  AACTTCCAGAGAAGCCATGGTTTTTTGTATTGGTCATAACTCAGCCCTTTGGGCGGCCTCTGGGCTTGGG
  CACCAGCTCATGCCAGCCCCAGAGGGTCAGGGTTGGAGGCCTGTGCTTGTGTTTGCTGCTAATGTCCAGC
  TACAGACCCAGAGGATAAGCCACTGGGCACTGGGCTGGGGTCCCTGCCTTGTTGGTGTTCAGCTGTGTGA
  TTTTGGACTAGCCACTTGTCAGAGGGCCTCAATCTCCCATCTGTGAAATAAGGACTCCACCTTTAGGGGA
  CCCTCCATGTTTGCTGGGTATTAGCCAAGCTGGTCCTGGGAGAATGCAGATACTGTCCGTGGACTACCAA
  GCTGGCTTGTTTCTTATGCCAGAGGCTAACAGATCCAATGGGAGTCCATGGTGTCATGCCAAGACAGTAT
  CAGACACAGCCCCAGAAGGGGGCATTATGGGCCCTGCCTCCCCATAGGCCATTTGGACTCTGCCTTCAAA
  CAAAGGCAGTTCAGTCCACAGGCATGGAAGCTGTGAGGGGACAGGCCTGTGCGTGCCATCCAGAGTCATC
  TCAGCCCTGCCTTTCTCTGGAGCATTCTGAAAACAGATATTCTGGCCCAGGGAATCCAGCCATGACCCCC
  ACCCCTCTGCCAAAGTACTCTTAGGTGCCAGTCTGGTAACTGAACTCCCTCTGGAGGCAGGCTTGAGGGA
  GGATTCCTCAGGGTTCCCTTGAAAGCTTTATTTATTTATTTTGTTCATTTATTTATTGGAGAGGCAGCAT
  TGCACAGTGAAAGAATTCTGGATATCTCAGGAGCCCCGAAATTCTAGCTCTGACTTTGCTGTTTCCAGTG
  GTATGACCTTGGAGAAGTCACTTATCCTCTTGGAGCCTCAGTTTCCTCATCTGCAGAATAATGACTGACT
  TGTCTAATTCGTAGGGATGTGAGGTTCTGCTGAGGAAATGGGTATGAATGTGCCTTGAACACAAAGCTCT
  GTCAATAAGTGATACATGTTTTTTATTCCAATAAATTGTCAAGACCAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001558.3
• Summary: 'The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]'
• Locus ID: 3587