MCCC2 (NM_022132) Human 3' UTR Clone

CAT#: SC216796

3`UTR clone of methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (MCCC2) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MCCC2
• Synonyms: MCCB
• ACCN: NM_022132
• Insert Size: 1858
• Sequence Data:
  Insert Sequence

  >SC216796 3'UTR clone of NM_022132
  The sequence shown below is from the reference sequence of NM_022132. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGCACCAATAGAGAAGACTGACTTCGGTATCTTCAGGATGTAACTGGAATAAAGGATGTTTTCTGTTGGA
  CATGTACTGAAAATTAACACATGTAGTAGCCTTAAAATTTTAGACTTCTCGAACATGAGGCTGTTACAGT
  AATTTTTTTAACACTGTGCATTGTACTTTTCTACCTTAAAAAAATCAGTGAGGATATTTATTTAATGAAC
  ATCAATTCCTTTTAAATTTTCTTAGAGAAATTTCTCTGTGGCTCAGTTTTACCACCCATAAAGCGGAGAC
  AGTAATTTACGGTTATCCTTTCTGACCCACAAAGTATGAAAAGTTCTGTAATCTGTAAACTCAGTTCTGT
  AATCTGTATTATTGAGATGATTAATATAAAGTTGTATTTTCACTGAAATGATTGTTTTGCTGGTTATGCT
  TGGTGATATTTTAGCGGGCTTATTTTTGAAAGGCATCTGTTACTTCAGTGGCATAAAGTGCCCTCACACT
  GCTGTGCAGCCATCACCACCATTCATCTCCAGAATTTGTTCTCAGTCCCAAACTGAAACTATACCATTCA
  AACAACAGCGCTCCCCATTTCCCCCTCCCCCGAGCCCATGACATCTCCTTATTATTATTTTTTTTTTGAG
  ACAGGGTGTTTCTCTGTTGCCCAGGCTGGAGTGCGGTGGTGTGATCTCGGCTCACTGCAGCTTCCACCTC
  CCGGGTTCAAGCGATTCTTGTGCCTCAGCCTCCCAAGCAGCTGGGACTATAGGCACGCACCACCACACCC
  AGCTAAGTTCTGTATTTTTAGCAGAGACGGGATTTCACTAGGTTGCCCAGGCTGGTCTCAAACTACTGAC
  CTCAAGTGATCCACCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCGCCTGACCA
  CATCTCCTTATTCTTAAATGTATGTGTTTCTCATGGTTTGATTTATTCTTGCTGGGCCATTTCAGCCCCT
  GTCATGGCTTCACTCGCCATCTAAATGCAGATGATTACTGCCTTGACGGTTCTTAACCCAGCTCTCCCCT
  GAGCTGCAGGCCTGCATATCCAGTAGGTCTACTGGACATCTGTACTGGTTGTTGTGGAGGAACCTCTGGC
  TTGCTCATTAAGTCCTACTGATTTTCACTATCCCCTGAATTTCCCCACTTATTTTTGTCTTTCACTATCG
  CAGGCCTTAGAAGAGGTCTACCTGCCTCCAGTCTTACCTAGTCCAGTCTACCCCCTGGAGTTAGAATGGC
  CATCCTGAAGTGAAAAGTAATGTCAGATTACTCCCTTCAGTGATTTCTTGTAGAAGTGCCAATCCCTGAA
  TGCCACCAAGATCTTAATCTTCACATCTTTAATCTTATCTCTTTGACTCCTCTTTACACCGGAGAACGGC
  TCCAGCTGTTCTAGCTCTCTTTCAGTTCTTTGAACCTTCCCACCTTAGGGTCTATAAGGTTCCCTCTGCC
  CAAATTGTTCTACTCTCCCTTCTTCTTCAACACATCCTTCAGTTTAAGCACTTGCTTCTCTCAGTTTAAA
  CTCCACTTCCTTAGGGATGTCTCTGTGACCTCCCTGTGCTGGATTAGCTTCCCTACTCTATGCTCTTGTA
  AAACACTATCTACTTCCTTTGTCATAAACTCATCATTTTCCTATAAACATTAATATTGTCCTCCTGCTAG
  AATGTAAGCTCCGTGAGAGCAAGGATCCTCCTGTTTACCCTGTACCTCCAATGTCTGGCACTTGTAGGTG
  CTCAAATATTCGTTGAATGAATGAAAAATCCATATTGTAATTGATGTCCTCTGGCCACATAGTTTTAAAA
  TTAGGTGATTGATTATATGACCGAATAGAACTATCAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_022132.4
• Summary: This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]
• Locus ID: 64087