CACNA2D2 (NM_001005505) Human 3' UTR Clone

CAT#: SC216802

3`UTR clone of calcium channel voltage-dependent alpha 2/delta subunit 2 (CACNA2D2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CACNA2D2
• Synonyms: CACNA2D; CASVDD
• ACCN: NM_001005505
• Insert Size: 1877 bp
• Sequence Data:
  Insert Sequence

  >SC216802 3'UTR clone of NM_001005505
  The sequence shown below is from the reference sequence of NM_001005505. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCAGCCTCAAGTCCTCGTCCACGCCTCTCGCCGCCTCTGAGCACCCTGCCCCACCCCACCTCCACTCCCA
  CCTCACCCGGCCTCTTCGCCTTTCCCACCCTCCTGCCCCACACTCCCCGCCTTAGAGCCTCGTCCCTCCC
  TCACTGAAGGACCTGAGCTGGCCAGGCCCTGAGAGTCTGGTCTGCGCCTTGGGATGGGGAGTCCCAAAGC
  GGGACGCCGCAGGTGTTTGGCACCCAAATCACATCTCACCTCCGAACTGTTCAAGTGTCCCCAGACCCTT
  CTTGCCTGCTGGGCTCCCCCCAGTGGGATGGGACAGGGAGGCCACACGCACTGGTGCCAAAACCAGGCCT
  CTGCTGCCGCCCTTCCTGGAGGCTGCCTATGTTGGGGGGGACCCTGCCTCAGCTGACCCGGCCTCTCTGC
  CCCACCCAAGCCCAAACTTGGTTTCTGTGAGAATAGTGGAGGAAGGTGAGATGGCCAGTTTGAAGCCTGT
  GCCTCCCAGCTTAAATCCTAGCAGGAGAGAGGCTCTGGGGCAGCCCCCATGGGCTCCTGCCCCTTTCAGG
  CCTACAGCCACATCCCCAAGCCCACCAGGTGTCAGGATAGTCACAGTGATACCAGTTCAGACACTACCCC
  ATATACACCTGGAACATTGAGGATGGAAACTGGACTCACATTCGACATACCCCACTGGGCACACGCACAA
  ACACACACACTATGGGGTGGGGTGGGTGTAGGGGCTTACAAAGCCTTACACAGGGCGAGGGGTTGGTGGG
  AGGGTTGGCACCTGCACACTCCATCTCCTGCTCACCACCTGCCTCTAATCTGAGCTGCAGCCTGGCTGGT
  CCTCCCATTTCTAAAGCTGAATGTCAAACAGTGCCAAATGCTGGGGCAGGGGGTGAAGAACCCTCTGTCC
  CACCCCTAGCCACCAGTGTCCTCCAAGTGCCCCCTCACCTCTCCAGGTGCTCATTGTAACCATTTCTCAC
  TAGTGTCAGGCCCCCAGTGGGACCACATGCCACTGCCTGCACCTTTCGGCAGAGGAACCCCCACCAGACA
  TCACCCTTTGCCTTAGCAGGGGTGACTTTGTCTCTCCTGGCTGGGCCATCCTTCCGCCAATCTGGCCCTT
  ACACACTCAGGCCTGTGCCCACTCCCTATCTCCTTCCCACCCCTACACACACACTCCCTGCTTGCAGGAG
  GCCAAACTGTCCCTCCCTTGCTGAACACACACACACACACACACACACAGGTGGGGACTGGGCACAGCTC
  TTCACACCATTCATTCTGGTCATTTCCCCCAAAGGCATCCCAGCCTGGGGGCCAGTGGGGAACTGAGGGC
  AAGGGGATATAGTGATGGGGCTCAGATGGACTGGGAGGAGGGGGAGGGTGATGCATTAATTAATGGCTTC
  GTTAATTAATGTCATGTTGCTTGTCGCTTTCTCAGTGTGTGTGTGTGGTCCATGCCCACTGCTGGTGCCA
  GGGTGGGTGTCCATGTGCACCCGGCCTGGATGCCAGCTGTGTCCTTCGGGGGCGTGCGTGTAACTGTAGT
  GTAGTCAGGTGCTCAATGGAGAATATAAACATATACAGAAAAATATATATTTTAAGTTTAAAAAACAGAA
  AAACAGACAAAACAATCCCCATCAGGTAGCTGTCTAACCCCCAGCTGGGTCTAATCCTTCTCATTACCCA
  CCCGACCTGGCTGCCCCTCACCTTGGGCTGGGGGACTGGGGGGCCATTTCCTTTTCTCTGCCCTTTTTTT
  GTTGTTCTATTTTGTACAGACAAGTTGGAAAAACAACAGCGACAAAAAAGTCAAGAAACTTTGTAAAATA
  TCGTGTGTGTGATTCCTTGTAAAATATTTTCAAATGGTTTATTACAGAAGATCAGTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001005505.1
• Summary: Calcium channels mediate the entry of calcium ions into the cell upon membrane polarization. This gene encodes the alpha-2/delta subunit of the voltage-dependent calcium channel complex. The complex consists of the main channel-forming subunit alpha-1, and auxiliary subunits alpha-2/delta, beta, and gamma. The auxiliary subunits function in the assembly and membrane localization of the complex, and modulate calcium currents and channel activation/inactivation kinetics. The subunit encoded by this gene undergoes post-translational cleavage to yield the extracellular alpha2 peptide and a membrane-anchored delta polypeptide. This subunit is a receptor for the antiepileptic drug, gabapentin. Mutations in this gene are associated with early infantile epileptic encephalopathy. Single nucleotide polymorphisms in this gene are correlated with increased sensitivity to opioid drugs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]
• Locus ID: 9254