Versican (VCAN) (NM_001164098) Human 3' UTR Clone

CAT#: SC216817

3`UTR clone of versican (VCAN) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: VCAN
• Synonyms: CSPG2; ERVR; GHAP; PG-M; WGN; WGN1
• ACCN: NM_001164098
• Insert Size: 1872 bp
• Sequence Data:
  Insert Sequence

  >SC216817 3'UTR clone of NM_001164098
  The sequence shown below is from the reference sequence of NM_001164098. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGAGTCGAGGCGCTGATCCCTAAAATGGCGAACATGTGTTTTCATCATTTCAGCCAAAGTCCTAACTTC
  CTGTGCCTTTCCTATCACCTCGAGAAGTAATTATCAGTTGGTTTGGATTTTTGGACCACCGTTCAGTCAT
  TTTGGGTTGCCGTGCTCCCAAAACATTTTAAATGAAAGTATTGGCATTCAAAAAGACAGCAGACAAAATG
  AAAGAAAATGAGAGCAGAAAGTAAGCATTTCCAGCCTATCTAATTTCTTTAGTTTTCTATTTGCCTCCAG
  TGCAGTCCATTTCCTAATGTATACCAGCCTACTGTACTATTTAAAATGCTCAATTTCAGCACCGATGGCC
  ATGTAAATAAGATGATTTAATGTTGATTTTAATCCTGTATATAAAATAAAAAGTCACAATGAGTTTGGGC
  ATATTTAATGATGATTATGGAGCCTTAGAGGTCTTTAATCATTGGTTCGGCTGCTTTTATGTAGTTTAGG
  CTGGAAATGGTTTCACTTGCTCTTTGACTGTCAGCAAGACTGAAGATGGCTTTTCCTGGACAGCTAGAAA
  ACACAAAATCTTGTAGGTCATTGCACCTATCTCAGCCATAGGTGCAGTTTGCTTCTACATGATGCTAAAG
  GCTGCGAATGGGATCCTGATGGAACTAAGGACTCCAATGTCGAACTCTTCTTTGCTGCATTCCTTTTTCT
  TCACTTACAAGAAAGGCCTGAATGGAGGACTTTTCTGTAACCAGGAACATTTTTTAGGGGTCAAAGTGCT
  AATAATTAACTCAACCAGGTCTACTTTTTAATGGCTTTCATAACACTAACTCATAAGGTTACCGATCAAT
  GCATTTCATACGGATATAGACCTAGGGCTCTGGAGGGTGGGGGATTGTTAAAACACATGCAAAAAAAAAA
  AAAAAAAAAAAAAAAGAAATTTTGTATATATAACCATTTTAATCTTTTATAAAGTTTTGAATGTTCATGT
  ATGAATGCTGCAGCTGTGAAGCATACATAAATAAATGAAGTAAGCCATACTGATTTAATTTATTGGATGT
  TATTTTCCCTAAGACCTGAAAATGAACATAGTATGCTAGTTATTTTTCAGTGTTAGCCTTTTACTTTCCT
  CACACAATTTGGAATCATATAATATAGGTACTTTGTCCCTGATTAAATAATGTGACGGATAGAATGCATC
  AAGTGTTTATTATGAAAAGAGTGGAAAAGTATATAGCTTTTAGCAAAAGGTGTTTGCCCATTCTAAGAAA
  TGAGCGAATATATAGAAATAGTGTGGGCATTTCTTCCTGTTAGGTGGAGTGTATGTGTTGACATTTCTCC
  CCATCTCTTCCCACTCTGTTTTCTCCCCATTATTTGAATAAAGTGACTGCTGAAGATGACTTTGAATCCT
  TATCCACTTAATTTAATGTTTAAAGAAAAACCTGTAATGGAAAGTAAGACTCCTTCCCTAATTTCAGTTT
  AGAGCAACTTGAAGAAGAGTAGACAAAAAATAAAATGCACATAGAAAAAGAGAAAAAGGGCACAAAGGGA
  TTGGCCCAATATTGATTCTTTTTTTATAAAACCTCCTTTGGCTTAGAAGGAATGACTCTAGCTACAATAA
  TACACAGTATGTTTAAGCAGGTTCCCTTGGTTGTTGCATTAAATGTAATCCACCTTTAGGTATTTTAGAG
  CACAGAACAACACTGTGTTGATCTAGTAGGTTTCTATTTTTCCTTTCTCTTTACAATGCACATAATACTT
  TCCTGTATTTATATCATAACGTGTATAGTGTAAAATGTGAATGACTTTTTTTGTGAATGAAAATCTAAAA
  TCTTTGTAACTTTTTATATCTGCTTTTGTTTCACCAAAGAAACCTAAAATCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001164098.1
• Summary: 'This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]'
• Locus ID: 1462