B3GALNT1 (NM_033169) Human 3' UTR Clone

CAT#: SC216849

3`UTR clone of beta-13-N-acetylgalactosaminyltransferase 1 (globoside blood group) (B3GALNT1) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: B3GALNT1
• Synonyms: B3GALT3; beta3Gal-T3; galT3; Gb4Cer; GLCT3; GLOB; P; P1
• ACCN: NM_033169
• Insert Size: 1896
• Sequence Data:
  Insert Sequence

  >SC216849 3'UTR clone of NM_033169
  The sequence shown below is from the reference sequence of NM_033169. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGTCATGCTAAGGAACACCACATGCCATTATTAACTTCACATTCTACAAAAAGCCTAGAAGGACAGGATA
  CTTTGTGGAAAGTGTTAAATAAAGTAGGTACTGTGGAAAATTCATGGGGAGGTCAGTGTGCTGGCTTACA
  CTGAACTGAAACTCATGAAAAACCCAGACTGGAGACTGGAGGGTTACACTTGTGATTTATTAGTCAGGCC
  CTTCAAAGATGATATGTGGAGGAATTAAATATAAAGGAATTGGAGGTTTTTGCTAAAGAAATTAATAGGA
  CCAAACAATTTGGACATGTCATTCTGTAGACTAGAATTTCTTAAAAGGGTGTTACTGAGTTATAAGCTCA
  CTAGGCTGTAAAAACAAAACAATGTAGAGTTTTATTTATTGAACAATGTAGTCACTTGAAGGTTTTGTGT
  ATATCTTATGTGGATTACCAATTTAAAAATATATGTAGTTCTGTGTCAAAAAACTTCTTCACTGAAGTTA
  TACTGAACAAAATTTTACCTGTTTTTGGTCATTTATAAAGTACTTCAAGATGTTGCAGTATTTCACAGTT
  ATTATTATTTAAAATTACTTCAACTTTGTGTTTTTAAATGTTTTGACGATTTCAATACAAGATAAAAAGG
  ATAGTGAATCATTCTTTACATGCAAACATTTTCCAGTTACTTAACTGATCAGTTTATTATTGATACATCA
  CTCCATTAATGTAAAGTCATAGGTCATTATTGCATATCAGTAATCTCTTGGACTTTGTTAAATATTTTAC
  TGTGGTAATATAGAGAAGAATTAAAGCAAGAAAATCTGAAGTATTGTCTTGTTTTTAAAAAATACAGTTC
  CTAGTGTTTTTAGAAGTCACTTAATTTGTCTCATTTTTCCACCTGGAAATTAGGAATAATGTAGAATGCA
  AGGCAGTAATTTCCTTTTGGAAAGGACTCTGAAGGCAGAAAAGAAGGGAGAGAACCTCATGGGCAGAATA
  TTATAAAAAGAGTGTCATATTCCAGCATTTGAATTGGAAAGAGAAGAGTGAAGATCCAAGTTGCATTATT
  AATCTGCCCTGTGTTTTTTCCTTTTAACAATCAGTTTGAGCTGCTGCTGTTATGAGTTTCTCATCAAGAT
  GAAAGCCCTAATATGTAAAGTCAAATCCGATTTAAATTTTGTGCTTTTATAGAAAGAAATTTCTTCATAG
  ACGTGGTGATATATCATTTGTTGGACCTGCTAATAGTAGGTCAAAGGGGAGCACTCCTTGCCCCCTGTTC
  CTGGGTTTATGCAGTTTTCTTTTTAGAGTTTATATAGGGCAAGTGGTTCTTTTTCTCTGAATTACAGGAT
  GGAAAAAGGTCATATCCTTTGTCAGGAAATATAAACTTGAAAGTATGTAGTCAGCTCTTGTAATACTCAT
  ATTTATGATTGTCCTATATGAAAAACAACTTCAGTTAAAACTATAATGTGTGATTCTGTATAACAAGGTG
  ATGTCTGTTTCCCAGGGCTCAGACCTAATCCAGTTATAATAAAATCAATTAAATGAAATATTCTATAGAA
  TCGATCTATGCCCTTGTTAATCTCCATCCATATAGGAGTCACGTTCTTTAAGACAGATGGTGGTAGTTAT
  TTTTGTGGCATGGTTAGATTTGACTGGTTTTGCAGAAGATTACAGTTATGTACTGCATAATGACATATAC
  AATAGTGGTCCCATAAAATTATAATGGAGCAGAAAATCTATTGCCTCATGATGTTTTAGCCGTTTTAATG
  TCATAGCCTAGTGCATTACTCACGTGTCTGTGGAGATGCTGGTGTAAACAAACCTACTACACTGCCAGTT
  GTATAAAAGTACAGCACATTCAGTTATGTACAGTATGTAATACTGATAATGACAATAAATGACACCGGTT
  TGTGTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_033169.2
• Summary: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). The encoded protein of this gene does not use N-acetylglucosamine as an acceptor sugar at all. [provided by RefSeq, Mar 2017]
• Locus ID: 8706