CHRNA2 (NM_000742) Human 3' UTR Clone

CAT#: SC216875

3`UTR clone of cholinergic receptor nicotinic alpha 2 (neuronal) (CHRNA2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHRNA2
• ACCN: NM_000742
• Insert Size: 1902 bp
• Sequence Data:
  Insert Sequence

  >SC216875 3'UTR clone of NM_000742
  The sequence shown below is from the reference sequence of NM_000742. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCTTTCTGCCTCCGTTCCTAGCTGGAATGATCTGACTGCACCTCCCTCGAGCTGGCTCCCAGGGCACCCC
  CGCTGACCATCTTTTGACCGTCTCTGCTACAGCTGCCTCTGGTGTCCCCTTTGGGATGAGCAGGTGCCTC
  TCCGCGGAGTCTGGACACTGACCCAGGGATTGCAGGCTTCCATCCAGCTTAACGTTATTGTACCAGGGCT
  GACAGCCTGCTGGGTGCAAAGCCCTGTTTTGGATACGGAGGAGCTGGGGAAGCTCTGCAGAAGGTCGCAA
  TAGTGTGGTGACCAAGTGGGGCAGAGTAAGATTCTCCTGGAGGAGCAGTGCAGACCCTAAGTGACAGAAG
  GGTCAACCAGGGGGACAGTGGGGCCAGGGTGACGAGGAAGGGAATGCAGACCTCAAAGGAGGGGCCGTGG
  GGGCAAAGGGGAGGGTTCTTGGATGTGGAAGGGCTTTGAACAATGTTTAGATTTGGAGATGAGCCCAAAG
  TGCCAGGGAGAACAGCCAGGTGAGGTGGGAGGTTGGAGAGCCAGGTGAGGTCTCTGTAAGTCAGGCTGGG
  GTTGAAGTTTGGAGTCTGTCCGAGTTTGCAGGGTGCTGAGCTGTATGGTCCAGCAGGGGAGTAATAAGGG
  CTCTTCTGGAAGGGGAGGAAGCGGGAGGCAGGGCCTGCACCTGATGTGGAGGTACAGGGCAGATCTTCCC
  TACCGGGGAGGGATGGATGGTTGGATACAGGTGGCTGGGCTATTCCATCCATCTGGAAGCACATTTGAGC
  CTCCAGGCTTCTCCTTGACGTCATTCCTCTCCTTCCTTGCTGCAAAATGGCTCTGCACCAGCCGGCCCCC
  AGGAGGTCTGGCAGAGCTGAGAGCCATGGCCTGCAGGGGCTCCATATGTCCCTACGCGTGCAGCAGGCAA
  ACAAGAAAGACCATCCTGAGCTGCTCCTGACCCACCTCAAACTCATCTCATTTGGCCTGTCCTCCCTCCC
  TTCACCCCTCCTTCCCTCCCCAGGAACCCTGTTCCCTGTGCCTGGCTCCATCAGGAGGAGGCCTCACTTG
  GGAGTTGAAGATCTTTGCCTTTAGTTCCTTTGATTCTGAACTTCTCAAAGGCAGGTACTCTGCCTGTTAT
  TTTTCCCACCCCCAGCACCTAGCACAGCGCCGGGTGAGTGGGGTCAGCACTTAGGGCGTTTGTTCTGAGT
  GGTTGAGGGGAGCAAGCTGGATTGTTTGGGCCCCAGGACTGTTGTGTAGATGGCAAAGCTGTGGTCCCGT
  CCCTTATCAGCCTGCCTCGTCTCTCCCAGAGCCCCGAGGTGTGTTGACCTGCTCTGAACCCTTCAGAGCA
  GCCTGTGTGTGCACCTTGGAATGCAATGCACCACCCCACAGTCTGAGGCTGCACCCAGGATCCCAGGCCG
  AGCTGCTGCTCCTGGGATCGGGCAACAGACCTCCCCCCAACTCTGTCCAGACTGGAGGGTGGGACATTGG
  CAGCTAACAGATGTGGGCATCGGGTTCTCGGGACAGCATGGAGGCTCCAGGCAGGAAGCCGAGCAGGGCG
  AGCTTCCAGTTTCCTTTTGGTCCTAGGGGTCCCCTCCTTCCAAACCCCAAAATGTCTCTCCCTGACACTC
  TCAGGGGAAACCAGCCTGGCTGTGACGCCCTGGCCCAGGCCCTGCCATGCCTCACTTCTCTCCCACAACC
  CCCCTGCTCATGGCACATCTGGTCTCTGGCTCCTGTCTACTCGTGTGCATGCAGCCCCCAGCGCCCCCTC
  TCCTCCCACTTGCTCTCCGGAGGCCTCAGAGCTCTCGGGTCCAGGCTCCCAGACCCACTCTCCTGAGACA
  GTGATGTTCACTTCTGTTATCCATGTCTCCCATCCATCTTGCATGTGAATAAAGAGATTAAATAAAGCTG
  CACCTACTGCTG
  
  CGGACCGTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000742.3
• Summary: 'Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq, Nov 2009]'
• Locus ID: 1135