G protein alpha inhibitor 1 (GNAI1) (NM_002069) Human 3' UTR Clone

CAT#: SC217016

3`UTR clone of guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 1 (GNAI1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GNAI1
• Synonyms: Gi
• ACCN: NM_002069
• Insert Size: 1921
• Sequence Data:
  Insert Sequence

  >SC217016 3'UTR clone of NM_002069
  The sequence shown below is from the reference sequence of NM_002069. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TAATCTAAAAGATTGTGGTCTCTTTTAAGTTTTGCAGTTCATGGTAAAATGCATTTTCAAACCAAATGAG
  TACTTATATATGGATCTCTGTAGACTAGAGTCTTGCAGCAACACAGAATGTAATATAAGGCAAATGCATC
  TGGGACTTGACCAAAGTTGTTCTGTTTTGTTTTTTTAACTGAAAGTAACAGAAGGACCTTTCTTAAATGT
  GACAGATGGTCCTGCAGTGTGAAACTGAAGGACAGTGTTAAAGCTGGGCTCTAGTATATTGATGATTTCT
  GCATAAGTGTAAATATGCAAATGTATGTATACATGTATTTATGACTTTAGTTTTCGACATTATTTTTAGG
  TTTTAAGAGTGGCAACTTAGGATTTTAGGGTGATGGCTTTGGAAATAACATAAATATACCTTGTACTGAA
  TGACAGACTATTACTACGTTTGCCAGTTTTAAACAGCTTTATTTATGTTCATGTCCTGTAAATTTTTAAG
  TACAGTAATTAATATTAGGAAACATTACAGCCCTTATCTAGATTATATGTATACTTGTATTAATAAAAAT
  GTTATTTGTACAAACATTGCACAGACTATTTTAATAACATGATTTGTTCTTTAAATTTTATGTGTTTTAT
  TGAAATGTTCTTAAGATGAATACACCTGCCTTTGGATCAACTATTTAAACATTGTATGCATTTTGATTTT
  TCCTACTTTAAGAAAATAAAATAATTTAATTTTACATTAGATTCCACGTTAGATTTGGTTTGAAAAACTA
  AAATTTCAGATTTCTGAGGATATACTGTCTTAGACTTATTGTACACACTTAGTTTTTATTCACTTGTTTT
  CACTCTGAATTTTAATATTTGGCTGATATGAATGCATTGCCTCAAAGGTGATGTCATCTTAATTTTTATT
  CACTTTAAATAACTACATTTTTGTTTATAACTAAGTTTGGAGGGATCCTAAGAGCATTTTTGTGGGTAAA
  AAAAAAACCTGTGGACATAATGAATTTTGAGACATTGATTGGTGAGGCTTTTATTTCCCTTGAGGAGTCT
  CTTGTACCTAGCATACATGATAGCTCCTTGTTGGGAAGATAACAAGAAGGATCTTTGAATACTCTATTGC
  TGATATAATGCAAGATTTAAATTTATACATATAACTAATTTCAAATGTAATTATCACACTATGTTAAAAT
  TACTTTTTTCCCTTAGATAATTCAAATTTCTCCACTTGCTTGAGATTATATCATTTCTTTTTCAATTATA
  CTATTATTTCTGAGAATGAAATGGACGATTACACTTAGAAAATGAGTAATAGTGTTTAATAAGTCAGTGA
  TTATATGTGTGCTCAAATAAGTGTTATGTATCAGCTAGATACTGAGCTTTGATAGAATAATTTTCTTTTG
  ATTATTCATGATGTGTCATCTCTGACCTTGTTTCAGCAAAGTAAACAGCACTCCCCACCCCACCCTCCTT
  TTTTTACTCATCTTGGAAAAGGTTAGTCTTTCAGTACACGTTGCTGGTAAGTAGTTTCCAAGTTACGTGT
  TGTCACTGGGTTGAAGTATATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAACCATAAACTATAT
  TCATATCTGTTTCATTTGGAGGATTTTCTTCTTTGTAATGTAAAGAAATTCAAAGTTATCAAAGTTCCTT
  AAATGTGTTAGTTTAGATTCTTTATGTGCCTTTCATGAAAGATATGTTTTCATTAATTTTACTGGTGGAC
  CTGTAATATCCACATTGTGAAGCTGTGTATGAAATTCAACTATAATATGAATAAATTTGAATCATGAGAA
  TTATGGGTTAAAAAGCCACAAAGAAGCACATATTGGTGACCATCATTAATGAAATCCTGAACTTTATTCT
  GTGTAATTGTGTTAATAAATCCTAATAAATT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_002069.5
• Summary: Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
• Locus ID: 2770