EYA1 (NM_172059) Human 3' UTR Clone

CAT#: SC217044

3`UTR clone of eyes absent homolog 1 (Drosophila) (EYA1) transcript variant 4 for miRNA target validation

Reconstitution Protocol

USD 670.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol EYA1
Synonyms BOP; BOR; BOS1; OFC1
ACCN NM_172059
Insert Size 1921 bp
Sequence Data
>SC217044 3'UTR clone of NM_172059
The sequence shown below is from the reference sequence of NM_172059. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

TGCCTTGGAACTGGAGTACCTGTAACAGCGCTCGGCACTTTGACAGCGCACAGCTGCTCTGTGACCAGGG
ACAGATCCAGCAGGCCCCAGTCTCGCATCAGCGCCGGCCTCCAGAACTTAGCAATTTCCGCCTGGTGATG
CGCAGTTGCTGTCAGTCTTGACCTCTGCCTTTGTGGTGAATGGAGGACCACGTCTATTTCATCAGAACAG
CTGTTGACTCTAGTACTGTGAATCCAGTGAAAATAAGCCATGAGAATGTTTTAGCACAGCGTTATGTGTC
TGCCACATTAACTACACGGTTCAAACCTGTGAAGAAAGGACCTGCAAACGCTTCAGTTGTTAGCATTTTC
AATGTGATATAAACAGCTTCTCCAATACAGCAAACCTAATTGCACAACAGAGACTGAAATGTGTTTCCTG
AATACCAGTGGAGGAATTTTCTTGTAAAGAAGGTTTACTTTTTGGTGTCTCATACCCAGGGTAATCTGTA
CATCTCTACTTATTTATGAACAGACTTTTTTTAAAAAGATAAAAAAACAGCTTTATTGAGGTATAATTCA
CCCACCAGACTTTTTTAAACATCAAATAATTGAAGAGACAATAGCATTAGAAATAAGTGATTAAAGGCCT
CTGCCTCACAACATGGCAAGTACAGTACTTTGAATTTTAGCACATTGCATAGTAGTTTTAAGTATGTCTA
ATTTAAACGTATAATATGTACATCACTGAGACAATCATGTACAGAAAGAATTTTTGGTGTAAATTTGTAA
TAATGGATAATTCTTTTACATATTGTTTAGGGAAATGATATTGAAAGGTAGCAATGCCTGGATAGTGAAG
CATGAGGCAGCACGTGCACAAATTCATGTGCCGTGCCTTATCTGAGTTTTCGGTATAAATATGTAGATAA
TGGATTTTTTTTTTTTAGATAATGTTGTCAAGACCAAAAGCATGGATGTCAAGTGTCAGTAAGGATTTTG
TTTTCTAAAATTTTTTCCTGCATCAGTTCTTCTGAGGGCCTTGATGAAATAACACAGCAGTTTCTTAAAC
AATTTGAAACAAAATGAGCTCTCCTACCACCTCACTTTTTCATTTCCACACTAATGTATTATATGTAACT
ACTTGGAAAAAATAATTATTCAAATGCTTCTTCCCACAAAGAATATAGATGATAGTAGATATATTTTATT
AATAAAATGGTTCATGAATCGGAGACTAACAAAGTTTTCATGTGCTCAGAATTATTAATTATCGTGTCTG
CATTTTCTTTCGATAAAGGAAGACACACGATGCTAATCCGGAAATCAGCAAACTTTGCATTACTCCCTAT
GTGCGTATTTTCTCTTTCTTCCTGTCACCCTGAGGAAGGTTCATTGCCATTGTCATCACCATGGAAACAA
CGTTCCTCTCCACCTGCATTATGTACTACATGACAGGCATCAATCTGGGGAAATAATAAAATTATCACCT
TTGTCAGACCATAAGAGTTTCTCCAAAAGTGGTCAGTTTGGCTGGGCAATATTTTCTCTCATCTAACAAA
CACAATCCATTGTCATGAAATTACCCTTAGGATGAGTCTTCTTTAATCAATCATATATTGGGCGGGAAAA
ACACCAGCTTTGACCCGAAGTAGTTGAAGAGCTACTTCATTCTTTTCTGAAGTTGTGTGTTGCTGCTAGA
AATAGTCATTTGTGAATTATCCAAATTGTTTAAATTCACAATTGAATTAGTTTTTTCTTCCTTTTTGCTT
GAAGCAAACAGTTGACAATTTTTAACCTTTTCATTTTATGTTTTTGTACTCTGCAGACTGAAAAGACAAA
GTTTATCTTGGCCTTACTGTATAAAGGTGTGCTGTGTCCACCGTTGTGTACAGAATTTTTCTTCATTAAT
TTTGTGTTTAAGTTAATAAAATTTATTTGTG

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_172059.2
Summary 'This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Dec 2013]'
Locus ID 2138

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.