TCF7 (NM_213648) Human 3' UTR Clone

CAT#: SC217085

3`UTR clone of transcription factor 7 (T-cell specific HMG-box) (TCF7) transcript variant 5 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TCF7
• Synonyms: TCF-1
• ACCN: NM_213648
• Insert Size: 1926 bp
• Sequence Data:
  Insert Sequence

  >SC217085 3'UTR clone of NM_213648
  The sequence shown below is from the reference sequence of NM_213648. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCCTTCCGATGACAGTGCTCTAGGCTGCCCCGGGTCCCCAGCTCCCCAGGACTCACCCTCATACCATCTG
  CTGCCCCGCTTCCCCACAGAACTGCTTACTAGCCCTGCGGAGCCGGCACCTACATCCCCAGGTCTCTCCA
  CTGCTCTCAGCCTCCCAACCCCAGGGCCCCCACAGGCCCCCCGCAGCACCCTGCAGAGCACACAGGTACA
  GCAACAGGAATCTCAGAGACAGGTGGCCTAGCAGGCACAGGACACCTGGCCGCCTCCAGGAGCCTACCCC
  CTGAAAGTGACAGAGACCCAGATCTCATGGAAACTGGCCAGGGGTCCTGTTAACGTCATCTCAGGGTCCA
  GACCCTGAAGATTTCAGAGGCTGCAGGACTTCTGCCTGAACCTGGGGTCATCGATTCAAACTGCTCCAAG
  TGGTGGGAATCAGATCTGTCTTGATGTGTCATCTAATTAAGGGAATCCCTTGTACCTATGGCTGCCTGCA
  TCTATTCTTTGTACCATCTGTCTTGCCAGCCAGAAGCCTCTGCCTCCCTAGCTTTTCTGCTATAGGTCAG
  AGATGGGCTGAACTGAGCCTAGCTACCTTCTCTACCCATCTCCCCCATCCCCCACTGCCACACCCTCCCC
  ATTCAGACACTTCATGGACCAAGAATGAGCTGGTTTGTCAAACAACATGTGAGCATGGTCACAAGCACAA
  AGCTCAAGATGACAGCTCTTCTAAGGAAATGGAGAAGCTCTGTTTATAAAAACAAAAACAAAACCAGCTG
  CTACTCATAAGTTGGACCAGAGGAAGCCCCTTACTATGATCTCAGGAGCTTGCAAGAAGCAGGAAGGGGA
  ATGGAATAGGTTAAGTTTAGGCCTATCAACCTAAGCAACAGAAATAATCTGACACTACCTTATCAGGCAA
  ATTGGGGAGGGGAGGGTGTATCTAGCTCTAGTTCAAATTATTTGGAAGTGTTCCCTGAGAAACCCACCAG
  CCTAAGAAGCTCTGGCCCCAGGCTTGTCACTAGCAGCTGCAGTCAACAGTTCAAAGAAGTCATGGCCCAA
  ATCCAGTGTGCACCCCTCCCCATTCACAGAGCCTTTTTCACAATTCCATTTCCAGTTCATCTATGGCAGT
  CCAGCCAGCTCCTGGGCAGCTTGAGAGGGCAAACCCAAAACCTCATGACAGCCAGAGCCTGTCTTTCAGC
  ATTCAGTCCGCCTGGCCGGCTCCAGTTTCCCCATGGGGCTGCGGGACAGAGGACCATTACAACTAGATCA
  AGGAGCCCAGAAAACCTCCAGTAGTGGACAACAGGTTTTCACCATAGCCTACGTTAACCCATTTTTGAGC
  CAAGCTTCAACCCTCAGCCTTGAAAAACAAGTCTTTAATTTAATTTTTGTTTTTTGCCTAAATCCAAAGA
  AAAAGGGCTGCCGGGCCAGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGCAGGCCGAGGCAGGT
  GGATCACCTGACGTCAGTAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCCTGTATCTACTAAAAATA
  CAAAAATTAGCCGGACGTGGTGGTGCGCGCATGTAATCCCAGCTACTCGGGAGGCTGAGGCGGAAGAATC
  ACTTGAACCCGGGAGGCGGAGGTTCCAGTGAGCCGAGATGGCGCTATTGCACTCCAGTCTGGGTAACAGG
  GAGACTGCATCTCAAAAAAAAAAAAAAAAAAAAAAAAGGGCTGTCCATGTATTCACACACCCCTCAAAAA
  AAGCCTTTAGTTCCTACTTTAGCCACTGGTTTCTCAGAATCCAAAGATCACATATTCTAGTGTAACACTG
  CAAGAAGTCTTGAGAAAAAGATTATTGTAGTGTTCAAAATATTTTTGTATTGTTAATGCATCATCATAGA
  AAAACTTTTAAACATGAGAATAAAGATACTTTTTAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_213648.3
• Summary: 'This gene encodes a member of the T-cell factor/lymphoid enhancer-binding factor family of high mobility group (HMG) box transcriptional activators. This gene is expressed predominantly in T-cells and plays a critical role in natural killer cell and innate lymphoid cell development. The encoded protein forms a complex with beta-catenin and activates transcription through a Wnt/beta-catenin signaling pathway. Mice with a knockout of this gene are viable and fertile, but display a block in T-lymphocyte differentiation. Alternative splicing results in multiple transcript variants. Naturally-occurring isoforms lacking the N-terminal beta-catenin interaction domain may act as dominant negative regulators of Wnt signaling. [provided by RefSeq, Oct 2016]'
• Locus ID: 6932